Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178179 2178181 3–1199 54 [0] [0] 54 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)33 (0.960) 23/90 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGC  >  W3110S.gb/2178182‑2178251
                                                                                                                                                 
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                            <  1:2484328/71‑1
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                            <  1:868202/71‑1
   CGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                           >  1:1789087/1‑69
   CGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                           >  1:1710845/1‑69
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                         <  1:1591208/70‑1
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                         <  1:1595477/70‑1
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                           >  1:2575010/1‑68
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                           >  1:91510/1‑68
     TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGC                                                                       <  1:2537101/71‑1
     TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGC                                                                       <  1:2208594/71‑1
     TTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGC                                                                       <  1:2582076/71‑1
      TATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                      >  1:1267231/1‑71
          ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAA                                                                  >  1:535960/1‑71
           TCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                         >  1:1298783/1‑63
              TCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                   <  1:1131367/66‑1
                CGCTTGCTGAAAATGAACTTTAATTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                 >  1:326036/1‑66
                     GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATTGCTGACGACTTAGCATGAATCGCCTCAC                                                         <  1:1910436/69‑1
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                    >  1:827158/1‑70
                          AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:1395068/1‑66
                           AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGT                                                 <  1:1570933/71‑1
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                            <  1:1029597/59‑1
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                            <  1:2560712/59‑1
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                               >  1:2556782/1‑55
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                 >  1:3587175/1‑53
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                 >  1:527493/1‑53
                                ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:1451680/71‑1
                                    TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                 >  1:194348/1‑46
                                     ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                        <  1:1402369/70‑1
                                         GTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                   >  1:1408002/1‑71
                                         GTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                               <  1:3222619/59‑1
                                         GTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                               <  1:20428/59‑1
                                          TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                  <  1:2257404/71‑1
                                                TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                             >  1:948863/1‑70
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:2592395/1‑42
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:1072689/1‑42
                                                   ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGT                                    >  1:1575026/1‑60
                                                     AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                       <  1:1086760/71‑1
                                                     AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                             <  1:2078766/65‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                        <  1:906155/53‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                        <  1:3257889/53‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                        <  1:514205/53‑1
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                     >  1:2117716/1‑71
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                     >  1:1763898/1‑71
                                                        TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                           <  1:1503381/48‑1
                                                         AAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:70823/1‑35
                                                         AAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                       >  1:1910001/1‑35
                                                            TGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCA                            >  1:3177600/1‑59
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                       <  1:533034/43‑1
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                       <  1:99827/43‑1
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                       <  1:3272327/43‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGG          <  1:2352446/71‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGG          <  1:2290081/71‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:650403/37‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:1417055/37‑1
                                                                   CGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                            <  1:2171507/36‑1
                                                                    GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                          <  1:1208202/53‑1
                                                                     ACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAAT       <  1:1801161/71‑1
                                                                     ACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA        <  1:2886248/70‑1
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG   >  1:2333143/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT    <  1:1583415/70‑1
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTC     <  1:585690/69‑1
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAG                                       >  1:1097208/1‑35
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGC  <  1:1688252/71‑1
                                                                                                                                                 
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGC  >  W3110S.gb/2178182‑2178251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.