| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,107 | A→C | 56.6% | E14A (GAA→GCA) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,107 | 0 | A | C | 56.6% | 192.2 / 161.7 | 159 | E14A (GAA→GCA) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (36/33); new base C (46/44); total (82/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
Only 100 of 161 total aligned reads displayed.
ACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTG > W3110S.gb/236043‑236175 | tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:131006‑M2/2‑71 (MQ=255)tCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:778568‑M2/2‑71 (MQ=255) aGTCTGACATAAATGCCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:2687040/1‑71 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacc < 1:1569766/66‑1 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:1310181/71‑1 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTAt > 1:1179559/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg < 1:3497549/69‑1 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:1445659/1‑71 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac < 1:2039662/59‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac < 1:3304728/59‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:710494/1‑69 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAcc > 1:399619/1‑70 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:1287763/1‑58 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:3381926/1‑58 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:1508856/1‑58 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACAGCAaccac > 1:1781532/1‑58 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:1345628/1‑55 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAg < 1:2975761/70‑1 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:2357676/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa > 1:570570/1‑69 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTa > 1:2519216/1‑60 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:355809/71‑1 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:308638/71‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:2810635/70‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:2175756/70‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg > 1:1837101/1‑71 (MQ=255) tATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:1986626/56‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:298307/61‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:1507451/1‑71 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt < 1:3652424/70‑1 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:1430097/1‑71 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa < 1:1918796/57‑1 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:1929646/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:619702/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3459523/71‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:2252280/1‑69 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:3221797/36‑1 (MQ=38) tGCAATTACACGCCAGATCGTTCTCGATACCGAAacca > 1:367168/1‑38 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:2928611/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACAAGATTGGTGCGCACTATGAAgg > 1:886102/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCa > 1:3372638/1‑61 (MQ=255) aaTTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:887832/39‑1 (MQ=38) aaTTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:1719089/39‑1 (MQ=38) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTg < 1:1931211/52‑1 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:1299917/1‑71 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:3209036/41‑1 (MQ=38) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:1014464/46‑1 (MQ=39) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc < 1:2558535/68‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:2933083/1‑42 (MQ=38) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:3013918/1‑60 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:1102695/1‑47 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:1747825/1‑63 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:1396514/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:363724/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:1755841/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:797105/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2307619/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:2644565/1‑46 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcaca < 1:3519001/70‑1 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:308840/1‑69 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:3177293/71‑1 (MQ=255) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:1649251/71‑1 (MQ=255) cacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:395867/60‑1 (MQ=255) cGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:205411/71‑1 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:1004515/71‑1 (MQ=255) ccAGATCGTTCTCGCTACCGCAACCCCCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATc < 1:83578/70‑1 (MQ=255) ccAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa < 1:1674635/71‑1 (MQ=255) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:1024491/1‑40 (MQ=38) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt > 1:637253/1‑71 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt > 1:877412/1‑40 (MQ=38) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:2743702/55‑1 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:2266333/71‑1 (MQ=255) aGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:3612955/71‑1 (MQ=255) gATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTg < 1:3292664/44‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:2901253/51‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:3134863/1‑55 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca < 1:2393204/59‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:2396305/71‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa < 1:137339/67‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa < 1:517795/67‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa < 1:1168951/67‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2092654/51‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2363089/51‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2581698/1‑68 (MQ=255) tCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag < 1:664927/71‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1912814/1‑67 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2150163/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2390760/1‑67 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga < 1:3176692/70‑1 (MQ=255) ttCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt < 1:784854/71‑1 (MQ=255) ctctATACCGAAACCACCGGTATGAACCAGATTGATGCGCAc > 1:1283432/1‑42 (MQ=38) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:850571/1‑48 (MQ=39) ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg > 1:2598438/1‑70 (MQ=255) tcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:84004/45‑1 (MQ=255) aCCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga > 1:617946/1‑59 (MQ=255) aCCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga > 1:2715974/1‑59 (MQ=255) cGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg < 1:3417037/71‑1 (MQ=255) gAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:1863063/1‑40 (MQ=255) gAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTg > 1:1078828/1‑70 (MQ=255) aaaCCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:3240550/48‑1 (MQ=255) | ACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTG > W3110S.gb/236043‑236175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |