| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,289,199 | C→T | 6.4% | T497M (ACG→ATG) | yejM → | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,289,199 | 0 | C | T | 6.4% | 198.3 / 3.4 | 62 | T497M (ACG→ATG) | yejM | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base C (34/24); new base T (2/2); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
ATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGATTTGTT > W3110S.gb/2289132‑2289265 | aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:799612/71‑1 (MQ=255) ttCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTg < 1:2296009/71‑1 (MQ=255) ttCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTg < 1:1921998/71‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGa > 1:3263574/1‑71 (MQ=255) cTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATg > 1:706122/1‑70 (MQ=255) cTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGc < 1:2926228/71‑1 (MQ=255) ggCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCaa > 1:2163291/1‑71 (MQ=255) gCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAAc < 1:2575776/71‑1 (MQ=255) aGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:2063886/60‑1 (MQ=255) ggCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCt < 1:1913701/71‑1 (MQ=255) aCGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGc > 1:2149924/1‑70 (MQ=255) aCGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGc > 1:2993069/1‑70 (MQ=255) aCGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGc > 1:1556443/1‑70 (MQ=255) aCGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGc > 1:1735983/1‑70 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTg < 1:1009832/54‑1 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCaa > 1:766457/1‑60 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACa > 1:1128454/1‑71 (MQ=255) gcgcAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGt > 1:2881044/1‑71 (MQ=255) cGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGca > 1:2580728/1‑70 (MQ=255) cGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGca > 1:2971945/1‑70 (MQ=255) tATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAg < 1:3145944/66‑1 (MQ=255) tATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAg < 1:3388586/66‑1 (MQ=255) tATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAg < 1:2845825/66‑1 (MQ=255) tATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCacac > 1:530036/1‑71 (MQ=255) ttAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGcaca > 1:1160712/1‑68 (MQ=255) ttAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt > 1:2280946/1‑71 (MQ=255) ttAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt > 1:535394/1‑71 (MQ=255) ttAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt > 1:1445073/1‑71 (MQ=255) tGCTCTGACTGATCATACCGATCTGATGACGATGCTGATGc < 1:1161466/41‑1 (MQ=255) tGCTCTGACTGATCATACCGATCTGATGACGATGCTGATGc < 1:2767110/41‑1 (MQ=255) gCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAg > 1:514960/1‑71 (MQ=255) ctctGACTGATCATACCGATCTGATGACGATGCTGATGCaa > 1:420682/1‑41 (MQ=255) ctctGACTGATCATACCGATCTGATGACGATGCTGATGCaa > 1:2180325/1‑41 (MQ=255) ctgactgaTCATACCGATCTGATGACGACGCTGATGc < 1:3580810/37‑1 (MQ=255) ctgactgaTCATACCGATCTGATGACGACGCTGATGc < 1:1194696/37‑1 (MQ=255) tgactgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGc < 1:119657/56‑1 (MQ=255) tgactgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGc < 1:3331331/56‑1 (MQ=255) tgactgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCg < 1:1773897/69‑1 (MQ=255) gactgaTCATACCGATCTGATGACGACGCTGATGCaa > 1:732458/1‑37 (MQ=255) gactgaTCATACCGATCTGATGACGACGCTGATGCaa > 1:2542923/1‑37 (MQ=255) actgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAata < 1:2860065/71‑1 (MQ=255) actgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAata < 1:2167815/71‑1 (MQ=255) ctgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAatat < 1:747599/71‑1 (MQ=255) ctgaTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAatat > 1:1165513/1‑71 (MQ=255) tgaTCAAACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATAtt > 1:473262/1‑71 (MQ=255) aTCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATAtt < 1:1657463/69‑1 (MQ=255) aTACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCa < 1:1145488/70‑1 (MQ=255) tACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCg < 1:2112087/59‑1 (MQ=255) aCCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCaa > 1:927255/1‑69 (MQ=255) aCCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCaa > 1:382603/1‑69 (MQ=255) ccGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGaa > 1:2849688/1‑59 (MQ=255) ccGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGt > 1:440068/1‑71 (MQ=255) tCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAatat > 1:3549501/1‑58 (MQ=255) tCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAatat > 1:1340422/1‑58 (MQ=255) tCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAata > 1:3332092/1‑57 (MQ=255) cTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGa > 1:608921/1‑71 (MQ=255) cTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGa > 1:811543/1‑71 (MQ=255) cTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGa > 1:831187/1‑71 (MQ=255) tgatgaCGACGCTGATGCAACGCCTGCTACATGTCAGca > 1:3068408/1‑39 (MQ=255) tgatgaCGACGCTGATGCAACGCCTGCTACATGTCAGca > 1:108464/1‑39 (MQ=255) atgaCGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAgg < 1:2225830/67‑1 (MQ=255) atgaCGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGAt < 1:3562481/69‑1 (MQ=255) gacgacGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCaa > 1:2490950/1‑58 (MQ=255) acgacgCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGATTTGtt < 1:3596592/71‑1 (MQ=255) gacgCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGATTTGtt > 1:1454484/1‑69 (MQ=255) | ATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCTGCCAGCGAATATTCGCAAGGTCAGGATTTGTT > W3110S.gb/2289132‑2289265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |