| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0% | 127.1 / NA | 44 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (32/12); total (32/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA > W3110S.gb/4563522‑4563656 | ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2900395/71‑1 (MQ=255)ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:229527/71‑1 (MQ=255) cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1130787/1‑71 (MQ=255) cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1143478/1‑71 (MQ=255) cGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:3108497/1‑71 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:2676128/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:3525386/1‑71 (MQ=255) gcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg > 1:3076905/1‑71 (MQ=255) agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:299391/1‑71 (MQ=255) gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:878075/1‑62 (MQ=255) gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:1820817/1‑70 (MQ=255) gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:2799990/1‑70 (MQ=255) ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:2275807/1‑71 (MQ=255) ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:2644859/1‑71 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:3195861/69‑1 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:1845676/69‑1 (MQ=255) aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2511986/69‑1 (MQ=255) tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATATGCTGTAACGCCATTTTa > 1:800378/1‑53 (MQ=39) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2337450/1‑71 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:2834616/1‑62 (MQ=255) cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:143439/1‑69 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:2141773/1‑44 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:1326170/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:1224826/1‑71 (MQ=255) tttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:1353313/1‑63 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:3196575/1‑71 (MQ=255) ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa < 1:2551707/46‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:2367943/40‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:950718/40‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:954721/40‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:3379573/60‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:2854941/1‑43 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa > 1:2755935/1‑62 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:2606943/1‑47 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg > 1:2396525/1‑61 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:1243907/1‑71 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:3408419/1‑52 (MQ=255) tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc < 1:1254438/71‑1 (MQ=255) tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc < 1:2743424/69‑1 (MQ=255) tAACGCCATTTTACTGTCCCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc < 1:2922817/71‑1 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:95875/1‑71 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa > 1:1112718/1‑59 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa > 1:486659/1‑57 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:769797/71‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa > 1:3211278/1‑71 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACCCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa > 1:424878/1‑71 (MQ=255) | GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA > W3110S.gb/4563522‑4563656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |