New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 3073461 =70 (1.290)3 (0.060) 3/90 NT 4.5% coding (391/510 nt) yggD predicted DNA‑binding transcriptional regulator
?W3110S.gb 3073499 = 64 (1.320)coding (353/510 nt) yggD predicted DNA‑binding transcriptional regulator
Rejected: Frequency below/above cutoff threshold.

GATTTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTATGACG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3073535‑3073461
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgacgTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATG  >  W3110S.gb/3073499‑3073566
                                                                                                                                               
GATTTGCCGATGACATGACCTTGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTAT                                                                          <  1:3925045/71‑1
GATTTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTAT                                                                          <  1:1271632/71‑1
GATTTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTAT                                                                          <  1:3242571/71‑1
GATTTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTAT                                                                          <  1:5061019/71‑1
 ATTTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTAT                                                                          >  1:820564/1‑70
   TTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTATGAC                                                                       >  1:34387/1‑71
                          TTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTATGACG                                                                      <  1:3900635/49‑1
                                  CAACGTCAATAGCATAACCCGCAATGCGATGCTCTAT                                                                          <  1:2457115/37‑1
                                             GCATAACCCGCAATGCGATGCTCTATGACGTTGCTGATAAAATCCCAGGTCATGTCATCGG                                       >  1:3260340/1‑61
                                                TAACCCGCAATGCGATGCTCTATGACGTTGCTGATAAAATCCC                                                      <  1:628585/43‑1
                                                  ACCCGCAATGCGATGCTCTATGACGTTGCTGATAAAATCCCAG                                                    >  1:430705/1‑43
                                                                      TGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCC                   <  1:2954274/56‑1
                                                                      TGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGG                              >  1:3803649/1‑45
                                                                       GACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAAT   >  1:1649676/1‑71
                                                                        ACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATG  <  1:2524346/71‑1
                                                                        ACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAAT   <  1:5134943/70‑1
                                                                          GTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCG                               >  1:3606293/1‑40
                                                                                                                                               
GATTTGCCGATGACATGACCTGGGATTTTATCAGCAACGTCAATAGCATAACCCGCAATGCGATGCTCTATGACG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3073535‑3073461
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgacgTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATG  >  W3110S.gb/3073499‑3073566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.