New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	3921545 =	73 (1.350)	3 (0.060)	3/88	NT	5.4%	coding (173/711 nt)	yiaF	conserved hypothetical protein
	?	W3110S.gb	3921580 =	41 (0.860)					coding (208/711 nt)	yiaF	conserved hypothetical protein

GCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3921620‑3921545 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tctgatTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  >  W3110S.gb/3921580‑3921646                                                                                                                                                 GCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTT                                                                          >  1:3853496/1‑71  CCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTT                                                                          >  1:865211/1‑70  CCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTT                                                                          >  1:1340792/1‑70   CTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCT                                                                        <  1:1977961/71‑1    TGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCT                                                                        >  1:2843268/1‑70    TGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTC                                                                         >  1:2799809/1‑69    TGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTC                                                                         >  1:4521859/1‑69      ATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGAT                                                                     >  1:1393706/1‑71      ATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTG                                                                       <  1:2389523/69‑1      ATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTG                                                                       <  1:3856921/69‑1      ATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTG                                                                       <  1:2771938/69‑1              GCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGAT                                                                     >  1:4176200/1‑63              GCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGAT                                                                     >  1:1021200/1‑63                            TACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGA                                                                      <  1:1509806/48‑1                             ACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGA                                                                      <  1:606601/47‑1                             ACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGA                                                                      <  1:1490021/47‑1                                 AATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGAT                                                                     >  1:1926980/1‑44                                           TCAGAGACAAAAGGACCAAACTGTTTTTTCTGATTACGCGATTCTGTATGGTTATTCTCAG                                          <  1:1985183/61‑1                                            CAGAGACAAAAGGACCAAACTGTTTTTTCTGATCACCCGATTCTGTATGGTTATTCTCAGCAGGTGAAT                                 >  1:978936/1‑69                                             AGAGACAAAAGGACCAAACTGTTTTTTCTGATTACGCGATTCTGTATGGTTATTCTCAGCA                                        >  1:1806785/1‑61                                                                         TGATTACTCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  >  1:3434949/1‑71                                                                         TGATTACTCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGA                          >  1:2893235/1‑47                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGT               >  1:3706304/1‑58                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGT               >  1:3069579/1‑58                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGT               >  1:461956/1‑58                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGA                          >  1:2392785/1‑47                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGA                          >  1:662039/1‑47                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATC                                >  1:3580448/1‑41                                                                         TGATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATC                                >  1:4321227/1‑41                                                                          GATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTC              >  1:4033489/1‑58                                                                           ATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  <  1:2591143/69‑1                                                                           ATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  <  1:2436001/69‑1                                                                           ATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  <  1:4630299/69‑1                                                                           ATTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  <  1:2245155/69‑1                                                                                                                                                 GCCTGATTCACCTGCTGAGAATAACCATACAGAATCGCGTAATCAGAGACAAAAGGACCAAACTGTTTTTTCTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3921620‑3921545 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tctgatTACGCGATTCTGTATGGTTATTCTCAGCAGGTGAATCAGGCGATGGATTCCGGTCTGCGCCCGGTTG  >  W3110S.gb/3921580‑3921646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.