Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 4310365–4311562 4311563 2–1199 72 [0] [0] 73 insH–[alsK] insH,[alsK]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 43103640 (0.000)52 (1.040) 27/92 NT 100% intergenic (‑286/‑69) yjcS/insH predicted alkyl sulfatase/IS5 element protein
?W3110S.gb 4311564 = 0 (0.000)coding (916/1029 nt) alsK D‑allose kinase

TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310292‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA  >  W3110S.gb/4311564‑4311631
                                                                                                                                             
TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTT                                                                         >  1:599307/1‑70
 ATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTT                                                                        <  1:4557755/70‑1
    TATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                    >  1:3361126/1‑71
    TATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTT                                                                         <  1:1027718/66‑1
      TTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCAC                                                                   <  1:1012743/70‑1
      TTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                    >  1:3217925/1‑69
       TGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                    >  1:2522407/1‑68
            CTGTCGTTGGCACATCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                            <  1:150114/71‑1
              GTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                 <  1:3077816/64‑1
               TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGT                                                           <  1:2842537/69‑1
                    GGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG                                                                      >  1:483828/1‑53
                         AGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGT                                               >  1:4404698/1‑71
                         AGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAG                                                                 >  1:3541324/1‑53
                         AGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCAC                                                                   >  1:4072473/1‑51
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              >  1:3909779/1‑71
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              <  1:4042588/71‑1
                          GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              <  1:2857142/71‑1
                           CGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTG                                                          >  1:4092480/1‑58
                             GGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              <  1:4999125/68‑1
                             GGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACT                                                             <  1:1767542/53‑1
                                  TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACT                                                             <  1:1003504/48‑1
                                  TTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAA                                                               <  1:2609293/46‑1
                                   TGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCC                                      >  1:4162212/1‑70
                                   TGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTG                                                          >  1:1608759/1‑50
                                    GCGCTACTGTTCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                    >  1:1352690/1‑71
                                    GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGCCAAAAAACGTTGATGCGCCAA                                    >  1:3956761/1‑71
                                       CTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATAT                                 <  1:4918113/71‑1
                                        TACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATT                                >  1:649757/1‑71
                                        TACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                    >  1:107221/1‑67
                                        TACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTG                                                          >  1:2161551/1‑45
                                           TGTGCCAACGTTTTACTCATGTGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCT                             >  1:2313112/1‑71
                                           TGTGCCAACGTTTTACTCATGTGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCT                             >  1:2319261/1‑71
                                           TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGTGCCAATATTGCT                             >  1:4100808/1‑71
                                           TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCT                             >  1:1658762/1‑71
                                           TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCT                             >  1:1263295/1‑71
                                               CCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                            <  1:551658/36‑1
                                                  ACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                      >  1:616708/1‑71
                                                  ACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                      >  1:757728/1‑71
                                                  ACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                      >  1:384396/1‑71
                                                   CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTG                     <  1:280852/71‑1
                                                   CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                      >  1:839473/1‑70
                                                   CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                      >  1:834489/1‑70
                                                    GTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCT                      >  1:3872786/1‑69
                                                    GTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAA                                                  >  1:4059645/1‑41
                                                     TTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                            <  1:3575166/46‑1
                                                     TTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                            >  1:1709746/1‑46
                                                     TTTTACTCATGGGGCTTTAGCACAGAACTGTGGCA                                                       <  1:1813977/35‑1
                                                     TTTTACTCATGGGGCTTTAGCACAGAACTGTGGCA                                                       <  1:3160551/35‑1
                                                      TTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                          >  1:84581/1‑47
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCG                 >  1:412857/1‑71
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATA                                  >  1:4053984/1‑54
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAAT                                   >  1:4048001/1‑53
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACG                                                >  1:4366335/1‑40
                                                       TTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACG                                                >  1:52772/1‑40
                                                        TACTCATGGGGCTTTAGCAGAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCG                 <  1:5074122/70‑1
                                                        TACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGC                >  1:1098604/1‑71
                                                          CTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                    >  1:4444663/1‑49
                                                          CTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              <  1:4121128/39‑1
                                                          CTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              <  1:1897020/39‑1
                                                          CTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                              <  1:1165792/39‑1
                                                             ATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACac                       >  1:3404395/1‑57
                                                             ATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCAT             >  1:827520/1‑69
                                                              TGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTA           <  1:659190/70‑1
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTGAAG        <  1:1258105/71‑1
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAG        <  1:3207380/71‑1
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                          >  1:2268402/1‑37
                                                                GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATG                                          >  1:3727454/1‑37
                                                                 GGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGT       >  1:3490013/1‑71
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG    >  1:963550/1‑69
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG    <  1:2932149/69‑1
                                                                      TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATA                                  >  1:4661364/1‑39
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA  <  1:5090932/70‑1
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA  <  1:2940827/70‑1
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA  <  1:3008951/70‑1
                                                                       AGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG    >  1:2326632/1‑68
                                                                                                                                             
TATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310292‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAA  >  W3110S.gb/4311564‑4311631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.