Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb547,6940AG100.0% 191.1 / NA 57pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE
ylbE
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG  >  W3110S.gb/547629‑547759
                                                                 |                                                                 
cgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                               >  1:2474222/1‑70 (MQ=255)
     cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac                                                         <  1:2944652/71‑1 (MQ=255)
     cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac                                                         <  1:697249/71‑1 (MQ=255)
     cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac                                                         <  1:703404/71‑1 (MQ=255)
      gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACt                                                        >  1:4146293/1‑71 (MQ=255)
          gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGATCTAAACGAGGGCAAAACACTGCt                                                     >  1:2853036/1‑70 (MQ=255)
          gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAGCTAAACGAGGGCAAAACACTGCtt                                                    >  1:2858885/1‑71 (MQ=255)
          gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                            >  1:3548769/1‑63 (MQ=255)
          gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                            >  1:719293/1‑63 (MQ=255)
          gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                            >  1:455012/1‑63 (MQ=255)
             cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc                                                 >  1:5089437/1‑71 (MQ=255)
             cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc                                                 >  1:5085257/1‑71 (MQ=255)
                      tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                               <  1:3187715/48‑1 (MQ=255)
                      tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCa                                                               <  1:4209396/48‑1 (MQ=255)
                        gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                       >  1:4336622/1‑70 (MQ=255)
                        gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                       <  1:3118214/70‑1 (MQ=255)
                         aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc                                       <  1:4487658/69‑1 (MQ=255)
                            tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc                                      <  1:3821603/67‑1 (MQ=255)
                            tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAAAACTGCt                                                     >  1:4756183/1‑52 (MQ=255)
                             gCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc                                 >  1:1789208/1‑71 (MQ=255)
                                  cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                            >  1:5181483/1‑71 (MQ=255)
                                  cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                            >  1:1196960/1‑71 (MQ=255)
                                   ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                              <  1:2722598/36‑1 (MQ=255)
                                    gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCa                          <  1:1874434/71‑1 (MQ=255)
                                    gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCa                          <  1:327574/71‑1 (MQ=255)
                                           cACTTATCAGCGAACTAAACGAGGGCAAAACACTGc                                                      >  1:3162321/1‑36 (MQ=255)
                                           cACTTATCAGCGAACTAAACGAGGGCAAAACACTGc                                                      >  1:3281570/1‑36 (MQ=255)
                                            aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATg                   <  1:3802984/70‑1 (MQ=255)
                                            aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATg                   <  1:841110/70‑1 (MQ=255)
                                            aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa                  >  1:1916078/1‑71 (MQ=255)
                                               tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg               >  1:1140720/1‑71 (MQ=255)
                                                aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                    >  1:5080994/1‑49 (MQ=255)
                                                aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCa                                     >  1:3418862/1‑48 (MQ=255)
                                                 tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCgg              <  1:4036110/70‑1 (MQ=255)
                                                   aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc           >  1:2832621/1‑71 (MQ=255)
                                                      gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                    >  1:2731042/1‑43 (MQ=255)
                                                       aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCTTg       <  1:859922/71‑1 (MQ=255)
                                                       aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg       <  1:2986771/71‑1 (MQ=255)
                                                        aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgag                      <  1:1783264/55‑1 (MQ=255)
                                                        aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg       <  1:5021816/70‑1 (MQ=255)
                                                        aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa      <  1:2562580/71‑1 (MQ=255)
                                                        aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCACGAGATGACCGGACCCATGa      <  1:405175/71‑1 (MQ=255)
                                                         cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt                              >  1:4012927/1‑46 (MQ=255)
                                                         cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt                              >  1:4988185/1‑46 (MQ=255)
                                                         cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc                           <  1:1133101/49‑1 (MQ=255)
                                                         cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                       <  1:2800157/53‑1 (MQ=255)
                                                          tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc                           <  1:4842362/48‑1 (MQ=255)
                                                          tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa                  <  1:3758363/57‑1 (MQ=255)
                                                           aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCTCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   >  1:3495298/1‑71 (MQ=255)
                                                           aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                            <  1:5151732/46‑1 (MQ=255)
                                                           aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa    <  1:4494820/70‑1 (MQ=255)
                                                           aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa      >  1:346388/1‑68 (MQ=255)
                                                            aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa    <  1:42463/69‑1 (MQ=255)
                                                            aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg  >  1:4899710/1‑71 (MQ=255)
                                                            aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   <  1:2343380/70‑1 (MQ=255)
                                                             aCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa      <  1:2612250/66‑1 (MQ=255)
                                                              cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa      >  1:1311752/1‑65 (MQ=255)
                                                                 |                                                                 
CGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG  >  W3110S.gb/547629‑547759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: