Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,740 T→G 66.4% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,7400TG66.4% 146.8 / 44.7 71G118G (GGT→GGGaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (10/14);  new base G (21/26);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC  >  W3110S.gb/70671‑70810
                                                                     |                                                                      
ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtt                                                                       >  1:3157725/1‑71 (MQ=255)
  tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttc                                                                     >  1:1406949/1‑71 (MQ=255)
    tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctt                                                                   >  1:5132390/1‑71 (MQ=255)
    tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt                                                                   >  1:5039995/1‑71 (MQ=255)
    tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                    <  1:1892477/70‑1 (MQ=255)
     aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACGGGCCGTCAATATTTGCCAATACGGGtttctt                                                                   <  1:3177700/70‑1 (MQ=255)
      cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctttc                                                                 >  1:4088715/1‑71 (MQ=255)
      cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttc                                                                 >  1:114952/1‑71 (MQ=255)
       tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                    <  1:2689598/67‑1 (MQ=255)
         tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc                                                              >  1:2486511/1‑71 (MQ=255)
         tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc                                                               >  1:3542089/1‑70 (MQ=255)
          cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGccc                                                             >  1:966072/1‑71 (MQ=255)
           gTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCg                                                            <  1:5086882/71‑1 (MQ=255)
                      tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa                                                 >  1:5157276/1‑71 (MQ=255)
                       aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                                >  1:4068574/1‑71 (MQ=255)
                        cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc                                                >  1:3435681/1‑70 (MQ=255)
                          ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAg                                             >  1:1313438/1‑71 (MQ=255)
                           gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc                                            <  1:4097711/71‑1 (MQ=255)
                           gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc                                            <  1:4103455/71‑1 (MQ=255)
                            cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc                                           <  1:1518026/71‑1 (MQ=255)
                             aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc                                           >  1:3622924/1‑70 (MQ=255)
                             aTGAATGGCTTAACTCGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCg                                          <  1:2882409/71‑1 (MQ=255)
                              tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct                                                                    <  1:3939290/44‑1 (MQ=255)
                              tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc                                                  <  1:768596/62‑1 (MQ=255)
                              tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg                                                   <  1:4265624/61‑1 (MQ=255)
                              tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg                                                   <  1:4259579/61‑1 (MQ=255)
                                aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                       >  1:900340/1‑71 (MQ=255)
                                aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCAGGATGAAGCGCa                                                 >  1:3349398/1‑61 (MQ=255)
                                aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAg                                             >  1:11270/1‑65 (MQ=255)
                                aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTAGCCCGGATGAAGCGCACCAGCCGCAt                                       >  1:3345057/1‑71 (MQ=255)
                                  tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:1959286/71‑1 (MQ=255)
                                  tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:3396591/71‑1 (MQ=255)
                                  tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt                                       <  1:1959271/69‑1 (MQ=255)
                                   ggCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa                                                 >  1:1029434/1‑58 (MQ=255)
                                    gCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt                                                                   >  1:1304214/1‑39 (MQ=255)
                                    gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     >  1:3457467/1‑69 (MQ=255)
                                    gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa                                   <  1:1979651/71‑1 (MQ=255)
                                        aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg                                <  1:4087663/70‑1 (MQ=255)
                                        aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa                                                          >  1:2318851/1‑44 (MQ=255)
                                          cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAcc                             >  1:3582633/1‑71 (MQ=255)
                                           tGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc                                    <  1:2551948/63‑1 (MQ=255)
                                             gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                           <  1:1307614/70‑1 (MQ=255)
                                              ccGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt                         <  1:3319778/71‑1 (MQ=255)
                                               cGTCAATATTTGCCAATACGGGTTTCTTTCTCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt                         <  1:1201635/70‑1 (MQ=255)
                                               cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                        >  1:391850/1‑71 (MQ=255)
                                               cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                        <  1:1768042/71‑1 (MQ=255)
                                                 tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg                                                        <  1:5131617/37‑1 (MQ=255)
                                                   aaTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa                                   >  1:950933/1‑56 (MQ=255)
                                                   aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:1173074/54‑1 (MQ=255)
                                                   aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:841448/54‑1 (MQ=255)
                                                   aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:1066292/54‑1 (MQ=255)
                                                   aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:250536/54‑1 (MQ=255)
                                                     tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa                  <  1:5091598/71‑1 (MQ=255)
                                                     tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa                  <  1:5085664/71‑1 (MQ=255)
                                                     tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt                                      <  1:2897335/51‑1 (MQ=255)
                                                     tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa                   >  1:365727/1‑70 (MQ=255)
                                                       tttGGCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                >  1:5068671/1‑71 (MQ=255)
                                                       tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                >  1:1969013/1‑71 (MQ=255)
                                                       tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                >  1:1292359/1‑71 (MQ=255)
                                                       tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc                                            >  1:950477/1‑43 (MQ=255)
                                                       tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                        >  1:3906158/1‑63 (MQ=255)
                                                       tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                        >  1:1185901/1‑63 (MQ=255)
                                                       tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                <  1:446680/71‑1 (MQ=255)
                                                       tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                <  1:4294859/71‑1 (MQ=255)
                                                       tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt                >  1:687123/1‑71 (MQ=255)
                                                         tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     >  1:199791/1‑48 (MQ=255)
                                                           ccAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt            <  1:668273/71‑1 (MQ=255)
                                                           ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt            <  1:4886493/71‑1 (MQ=255)
                                                           ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt             <  1:3037708/70‑1 (MQ=255)
                                                              aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt                                      <  1:1571270/42‑1 (MQ=255)
                                                              aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                        <  1:2955840/56‑1 (MQ=255)
                                                              aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa                   <  1:224990/61‑1 (MQ=255)
                                                              aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc         >  1:2170697/1‑71 (MQ=255)
                                                              aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt                                     <  1:4352792/43‑1 (MQ=255)
                                                              aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg                           <  1:1383753/53‑1 (MQ=255)
                                                              aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc         >  1:872712/1‑71 (MQ=255)
                                                                aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc                                         >  1:391212/1‑37 (MQ=255)
                                                                aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa                                        >  1:3655675/1‑38 (MQ=255)
                                                                 cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc      <  1:564112/71‑1 (MQ=255)
                                                                 cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc      <  1:3462350/71‑1 (MQ=255)
                                                                 cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc      <  1:823055/71‑1 (MQ=255)
                                                                 cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc      <  1:1314380/71‑1 (MQ=255)
                                                                     tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt                          <  1:1239898/47‑1 (MQ=255)
                                                                     tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTa           <  1:863888/62‑1 (MQ=255)
                                                                     tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGc  <  1:2087558/71‑1 (MQ=255)
                                                                     |                                                                      
GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC  >  W3110S.gb/70671‑70810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: