Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 70,740 | T→G | 66.4% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 70,740 | 0 | T | G | 66.4% | 146.8 / 44.7 | 71 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
Reads supporting (aligned to +/- strand): ref base T (10/14); new base G (21/26); total (31/40) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70671‑70810 | ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtt > 1:3157725/1‑71 (MQ=255) tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttc > 1:1406949/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctt > 1:5132390/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt > 1:5039995/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:1892477/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACGGGCCGTCAATATTTGCCAATACGGGtttctt < 1:3177700/70‑1 (MQ=255) cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctttc > 1:4088715/1‑71 (MQ=255) cTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttc > 1:114952/1‑71 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:2689598/67‑1 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc > 1:2486511/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGc > 1:3542089/1‑70 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGccc > 1:966072/1‑71 (MQ=255) gTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCg < 1:5086882/71‑1 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:5157276/1‑71 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:4068574/1‑71 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCAc > 1:3435681/1‑70 (MQ=255) ggCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAg > 1:1313438/1‑71 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc < 1:4097711/71‑1 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc < 1:4103455/71‑1 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:1518026/71‑1 (MQ=255) aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGcc > 1:3622924/1‑70 (MQ=255) aTGAATGGCTTAACTCGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCg < 1:2882409/71‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:3939290/44‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc < 1:768596/62‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg < 1:4265624/61‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgcg < 1:4259579/61‑1 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt > 1:900340/1‑71 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCAGGATGAAGCGCa > 1:3349398/1‑61 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAg > 1:11270/1‑65 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTAGCCCGGATGAAGCGCACCAGCCGCAt > 1:3345057/1‑71 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:1959286/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:3396591/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAt < 1:1959271/69‑1 (MQ=255) ggCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:1029434/1‑58 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctt > 1:1304214/1‑39 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:3457467/1‑69 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa < 1:1979651/71‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:4087663/70‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:2318851/1‑44 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAcc > 1:3582633/1‑71 (MQ=255) tGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc < 1:2551948/63‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg < 1:1307614/70‑1 (MQ=255) ccGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:3319778/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCTCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:1201635/70‑1 (MQ=255) cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt > 1:391850/1‑71 (MQ=255) cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:1768042/71‑1 (MQ=255) tCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATg < 1:5131617/37‑1 (MQ=255) aaTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa > 1:950933/1‑56 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:1173074/54‑1 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:841448/54‑1 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:1066292/54‑1 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:250536/54‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:5091598/71‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCaa < 1:5085664/71‑1 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt < 1:2897335/51‑1 (MQ=255) tatTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa > 1:365727/1‑70 (MQ=255) tttGGCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:5068671/1‑71 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:1969013/1‑71 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:1292359/1‑71 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGc > 1:950477/1‑43 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt > 1:3906158/1‑63 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt > 1:1185901/1‑63 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:446680/71‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt < 1:4294859/71‑1 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:687123/1‑71 (MQ=255) tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:199791/1‑48 (MQ=255) ccAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:668273/71‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:4886493/71‑1 (MQ=255) ccAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAt < 1:3037708/70‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt < 1:1571270/42‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:2955840/56‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa < 1:224990/61‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc > 1:2170697/1‑71 (MQ=255) aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:4352792/43‑1 (MQ=255) aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTg < 1:1383753/53‑1 (MQ=255) aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc > 1:872712/1‑71 (MQ=255) aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGc > 1:391212/1‑37 (MQ=255) aCGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCa > 1:3655675/1‑38 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:564112/71‑1 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:3462350/71‑1 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:823055/71‑1 (MQ=255) cGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:1314380/71‑1 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:1239898/47‑1 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTa < 1:863888/62‑1 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGc < 1:2087558/71‑1 (MQ=255) | GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70671‑70810 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |