Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,461,546:1 +A 8.3% intergenic (‑454/+227) yfcV ← / ← sixA predicted fimbrial‑like adhesin protein/phosphohistidine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,461,5461.A 8.3% 613.3 / 57.8 156intergenic (‑454/+227)yfcV/sixApredicted fimbrial‑like adhesin protein/phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base . (72/71);  new base A (10/3);  total (82/74)
Fisher's exact test for biased strand distribution p-value = 8.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

TAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAT  >  W3110S.gb/2461480‑2461614
                                                                   |                                                                    
taGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTg                                                                    <  1:1021224/69‑1 (MQ=255)
     aGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATtaa                                                              >  1:5376154/1‑70 (MQ=255)
     aGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATtaa                                                              >  1:292708/1‑70 (MQ=255)
     aGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATtaa                                                              >  1:1815943/1‑70 (MQ=255)
           tAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGa                                                       >  1:1335253/1‑71 (MQ=255)
             aTCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGAt                                                      <  1:4874750/70‑1 (MQ=255)
                aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:3356251/70‑1 (MQ=255)
                aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:2624347/70‑1 (MQ=255)
                aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:2567751/70‑1 (MQ=255)
                aaGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:1663556/70‑1 (MQ=255)
                 aGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   >  1:4825695/1‑69 (MQ=255)
                    ttCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAgtg                                              >  1:5307921/1‑71 (MQ=255)
                    ttCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAgtg                                              >  1:3036264/1‑71 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTTTTTTCTCCATTATCTg                                                                    <  1:3679060/44‑1 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTg                                                                    <  1:3009304/44‑1 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTg                                                                    <  1:1000206/44‑1 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   >  1:1719436/1‑61 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   >  1:3149517/1‑61 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   >  1:693737/1‑61 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   >  1:4196019/1‑61 (MQ=255)
                         ttACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                         <  1:4311519/71‑1 (MQ=255)
                                ctgcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:2377616/54‑1 (MQ=255)
                                ctgcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACat                                  >  1:4286315/1‑71 (MQ=255)
                                  gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACatta                                >  1:4494524/1‑71 (MQ=255)
                                  gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACatta                                >  1:3709535/1‑71 (MQ=255)
                                  gcctgATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACatta                                >  1:658953/1‑71 (MQ=255)
                                       attattCAACTCTATTTTCTCCATTATCTGTATTAATaaa                                                          >  1:3200850/1‑40 (MQ=255)
                                       attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGa                                     >  1:2213932/1‑61 (MQ=255)
                                       attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCa                            <  1:2693216/70‑1 (MQ=255)
                                       attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCAt                           >  1:4808628/1‑71 (MQ=255)
                                       attattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCAt                           >  1:765758/1‑71 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATaaa                                                          >  1:1536445/1‑39 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATaaa                                                          >  1:872409/1‑39 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATaaa                                                          >  1:2459505/1‑39 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:2857057/46‑1 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:325440/46‑1 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:4235927/46‑1 (MQ=255)
                                        ttattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:3412005/46‑1 (MQ=255)
                                         tattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAAt                                                   <  1:2218769/45‑1 (MQ=255)
                                         tattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                         <  1:4514093/55‑1 (MQ=255)
                                         tattCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                         <  1:2100360/55‑1 (MQ=255)
                                           ttaaaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                        <  1:182247/67‑1 (MQ=255)
                                           ttCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                        <  1:4979840/70‑1 (MQ=255)
                                           ttCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                        <  1:5189329/70‑1 (MQ=255)
                                           ttCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                        <  1:1068602/70‑1 (MQ=255)
                                             cAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTc                        <  1:4019074/68‑1 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:5165177/1‑43 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:5310601/1‑43 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:4253619/1‑43 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                    >  1:570999/1‑71 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                    >  1:2753339/1‑71 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                    >  1:1223070/1‑71 (MQ=255)
                                              aaCTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACa                    >  1:554816/1‑71 (MQ=255)
                                                 tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCaa                      <  1:944629/66‑1 (MQ=255)
                                                 tctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCaa                      <  1:2520752/66‑1 (MQ=255)
                                                  ctATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 >  1:5374024/1‑70 (MQ=255)
                                                   tataTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:3903582/69‑1 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                         >  1:1493459/1‑45 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCaaa                                         >  1:4761688/1‑45 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:2106066/69‑1 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:2515618/69‑1 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:4859297/69‑1 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:4826089/69‑1 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:4808971/69‑1 (MQ=255)
                                                   tATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGt                 <  1:4023133/69‑1 (MQ=255)
                                                     ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:3935027/1‑36 (MQ=255)
                                                     ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:5492885/1‑36 (MQ=255)
                                                     ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:3289739/1‑36 (MQ=255)
                                                     ttttCTCCATTATCTGTATTAATAAATGATAATGAg                                                >  1:5051377/1‑36 (MQ=255)
                                                     ttttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTaa               <  1:1307124/69‑1 (MQ=255)
                                                     ttttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTaa               <  1:1220853/69‑1 (MQ=255)
                                                       ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt           >  1:2995844/1‑71 (MQ=255)
                                                       ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt           >  1:790459/1‑71 (MQ=255)
                                                       ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt           >  1:5470755/1‑71 (MQ=255)
                                                       ttCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAAAAGACATTATTCATTTCAACATGTAACtt            >  1:4674278/1‑70 (MQ=255)
                                                        tctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt           <  1:988045/70‑1 (MQ=255)
                                                        tctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt           <  1:2328211/70‑1 (MQ=255)
                                                        tctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACttt           <  1:2320443/70‑1 (MQ=255)
                                                         ctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTg          <  1:3388739/70‑1 (MQ=255)
                                                         ctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTg          <  1:2834562/70‑1 (MQ=255)
                                                         ctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTg          <  1:3073920/70‑1 (MQ=255)
                                                         ctcCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTg          <  1:1518749/70‑1 (MQ=255)
                                                          ctcATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCAtt                          <  1:105042/51‑1 (MQ=9)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:1180162/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:2488584/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:3491164/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:2664960/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:3256095/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:859672/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:796289/1‑53 (MQ=17)
                                                            cATTATCATTTATTAATACAGATAATGAGTGTCAAACAGACATTATTCATTTc                        >  1:1996156/1‑53 (MQ=17)
                                                              ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACt             <  1:762941/62‑1 (MQ=255)
                                                              ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACt             <  1:1427982/62‑1 (MQ=255)
                                                              ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACt             <  1:5203750/62‑1 (MQ=255)
                                                              ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCaa     >  1:4899016/1‑70 (MQ=255)
                                                              ttATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCaa     >  1:929270/1‑70 (MQ=255)
                                                                aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACtt            >  1:4267791/1‑61 (MQ=255)
                                                                aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAt  >  1:5166796/1‑71 (MQ=255)
                                                                aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAt  >  1:4659227/1‑71 (MQ=255)
                                                                aTCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAt  >  1:4060613/1‑71 (MQ=255)
                                                                   |                                                                    
TAGCCAGAATTTAATCAAGTTTCAATTACGTCCTGCCTGATTATTCAACTCTATTTTCTCCATTATCTGTATTAATAAATGATAATGAGTGTCAAACAGACATTATTCATTTCAACATGTAACTTTGGGCAAAAT  >  W3110S.gb/2461480‑2461614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: