Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262332 3262332 1–1199 92 [1] [0] 86 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)60 (1.120) 18/92 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401
                                                                                                                                               
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                          >  1:647302/1‑71
    TCTAGCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       <  1:4823198/70‑1
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                      >  1:3226609/1‑71
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       <  1:4768517/70‑1
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACT                                                                     <  1:1230458/68‑1
           TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:3869017/1‑70
           TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:2736630/1‑70
           TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:529076/1‑70
           TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCAGCGGGCGCGTTAACTTTGCC                                                                >  1:2952581/1‑70
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCT                                                               >  1:1501173/1‑70
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCT                                                               >  1:1968885/1‑70
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:4049626/1‑69
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:2503300/1‑69
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:4557602/1‑69
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAA                                                             <  1:1141581/71‑1
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAA                                                             <  1:223947/71‑1
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAA                                                             <  1:537180/71‑1
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                                    >  1:708778/1‑64
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATAATCCAAATTCTGCGGGCGCGTTAACTT                                                                    >  1:1551817/1‑64
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           <  1:4439981/70‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:4242617/65‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:4170413/65‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:1836748/65‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                                    >  1:4196590/1‑59
                    ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTACCTTTGCCTAAA                                                            >  1:1788755/1‑65
                    ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:381148/1‑65
                    ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:1580364/1‑65
                    ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:3786207/1‑65
                            TCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                      <  1:4788577/63‑1
                            TCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATG                                                      <  1:5361735/63‑1
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCA                                               <  1:3114045/68‑1
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:2671607/1‑63
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:3867055/1‑63
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:5437680/1‑63
                              TACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:4921927/1‑63
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:2442337/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:906826/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:1775704/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:3165498/1‑71
                                     CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                                     >  1:3613181/1‑71
                                      TTCAACTAAATTATGCAAATTCTGCGGGGGCGTTAA                                                                       >  1:3411158/1‑36
                                      TTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:2412202/1‑36
                                      TTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                                       >  1:3608052/1‑36
                                      TTCAACTAAATTATGCAAATTCTGCGGGCGCGTTA                                                                        >  1:127981/1‑35
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATC                                                 <  1:4233058/57‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATC                                                 <  1:3775036/57‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATC                                                 <  1:1754408/57‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATCAATGGCATC                                                 <  1:507860/57‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:491772/44‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:3054094/44‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:5429086/44‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:1354060/44‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:580446/44‑1
                                       TCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                              <  1:4178487/44‑1
                                        CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  >  1:1101095/1‑71
                                        CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATT                                    <  1:4431697/69‑1
                                        CAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATC                                                 <  1:4734876/56‑1
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:2757837/70‑1
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:5225591/70‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG                   <  1:2594775/71‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:4193291/59‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:5049730/59‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:2003132/59‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:5529304/59‑1
                                                          TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAG                       >  1:2175966/1‑64
                                                          TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGC                                                    >  1:3869689/1‑35
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:5454937/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2945793/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:479158/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:31408/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:2388798/70‑1
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:730503/70‑1
                                                                     GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAA     >  1:984309/1‑71
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:2060725/1‑71
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:920513/1‑71
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:938101/49‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:4537298/49‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          <  1:3877557/49‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:5349231/41‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:1572760/41‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:2247945/41‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:2428756/41‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                                  <  1:1570403/41‑1
                                                                       TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:5592454/1‑70
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTT                         >  1:941673/1‑48
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTT                         >  1:811120/1‑48
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:3558373/1‑47
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:277127/1‑47
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:2107138/1‑47
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT                          >  1:1851865/1‑47
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCG                           >  1:1951849/1‑46
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:1543289/70‑1
                                                                         ACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  <  1:1995772/70‑1
                                                                                                                                               
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.