New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 3738790126 (2.160)5 (0.100) 3/90 NT 4.0% coding (388/468 nt) yieI predicted inner membrane protein
?W3110S.gb = 3738797 124 (2.360)coding (381/468 nt) yieI predicted inner membrane protein
Rejected: Frequency below/above cutoff threshold.

TCAGGACATGAAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3738729‑3738790
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctgcATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAA  <  W3110S.gb/3738797‑3738728
                                                                                                                                    
TCAGGACATGAAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGCATTGCTGG                                                                >  1:1897714/1‑70
TCAGGACATGAAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGCATTGCTGG                                                                >  1:2603335/1‑70
       ATGAAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGCATTGCTGGCAGGTGTG                                                        <  1:1748670/71‑1
       ATGAAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGCATTGCTGGCAGGTGTG                                                        <  1:2917458/71‑1
          AAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGCATTGCTGGCAGGTGTGGTG                                                     <  1:4930833/71‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:2500840/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:2997550/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:3519072/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:3580199/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:4393177/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:5057352/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:673209/42‑1
                                                           TGCATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCG                                 <  1:793288/42‑1
                                                             CATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAA  <  1:146045/71‑1
                                                             CATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAA  <  1:682518/71‑1
                                                             CATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGA   <  1:3964752/70‑1
                                                                                                                                    
TCAGGACATGAAATATCCAATCCGGGTAGACGGGTAATGATTCTGCTCATCACCACACCTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3738729‑3738790
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctgcATTGCTGGCAGGTGTGGTGATGAGCAGAATCATTACCCGTCTACCCGGATTGGATATTTCATGTCCTGAA  <  W3110S.gb/3738797‑3738728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.