Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3746913–3748108 3748111 4–1199 105 [0] [0] 108 [tnaB]–[tnaB] [tnaB],insH,[tnaB]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 37469120 (0.000)94 (1.750) 23/92 NT 100% coding (71/447 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 3748112 = 0 (0.000)coding (871/984 nt) tnaB tryptophan transporter of low affinity

Only 100 of 118 total aligned reads displayed.

TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3746840‑3746912
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  W3110S.gb/3748112‑3748171
                                                                                                                                     
TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:3625674/1‑70
TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:1427610/1‑70
 TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:844547/1‑69
 TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:624691/1‑69
 TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:1956508/1‑69
 TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:453426/1‑69
 TAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:3725182/1‑69
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:2282912/1‑68
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:2884553/1‑68
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:3434792/1‑68
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:1764679/1‑68
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                 >  1:4185232/1‑68
    ACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAt                                                            <  1:612923/71‑2
    ACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                            <  1:4172696/71‑1
        GTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG                                                              <  1:2944004/65‑1
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                    >  1:3645183/1‑69
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                    >  1:1915855/1‑69
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                    >  1:3638643/1‑69
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                    >  1:730911/1‑69
                 ATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGG                                                >  1:495051/1‑70
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGaa                                              >  1:5229696/1‑68
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                              >  1:3422090/1‑70
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                              >  1:434506/1‑70
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                              >  1:4699596/1‑70
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                              >  1:2515145/1‑70
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                              >  1:980672/1‑70
                      CGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAAC                                          >  1:116004/1‑71
                       GAACAACCCCAGCGTGCCACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAAC                                          >  1:952565/1‑70
                        AACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTC                                        <  1:1967035/71‑1
                        AACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAAC                                                         <  1:4484426/54‑1
                         ACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGACCACCAGCAGGCAAAACTC                                        >  1:1486643/1‑70
                           AACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:1171453/71‑1
                           AACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:3267894/71‑1
                            ACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:2682673/70‑1
                               CCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                    <  1:2623797/52‑1
                               CCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                    <  1:5145058/52‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAGCACAGCAGGCAAAACTCGATAA                                   <  1:2382543/67‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:1957231/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:5038991/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:3499519/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:5286720/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:1499270/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:3370249/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:978554/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:689043/68‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:2561480/68‑1
                                 AGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCC                               >  1:5586885/1‑71
                                 AGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                >  1:2291660/1‑70
                                 AGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                >  1:899019/1‑70
                                 AGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:706524/67‑1
                                  GCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                            <  1:1756409/41‑1
                                    GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                   <  1:1295832/64‑1
                                          CCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTT                      <  1:1679401/71‑1
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGG                    >  1:2637463/1‑71
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:5595461/1‑55
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:4343773/1‑55
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:4260674/1‑55
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:4644170/1‑55
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:1721860/1‑55
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:1092107/1‑55
                                            AAAGAACGAACTGGCAACAGCTAAATTAGAGAACACCAGCAGGCAAAACTCGATA                                    >  1:3299759/1‑55
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGTCAAAACTCGAT                                     <  1:4558621/52‑1
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTG                  >  1:3241836/1‑71
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:3847997/52‑1
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:163665/52‑1
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:3451687/52‑1
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:1982715/52‑1
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:1017148/52‑1
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACCA                                                     >  1:2604936/1‑36
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:1315413/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:4689692/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:2329195/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:3629334/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:2264199/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:3135539/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:2836370/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGAGCC                >  1:2729491/1‑71
                                                 ACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:2526234/70‑1
                                                 ACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                <  1:3337956/70‑1
                                                 ACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTT                      <  1:3474815/64‑1
                                                     ACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGG            >  1:927087/1‑70
                                                     ACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCC                               >  1:768311/1‑51
                                                       TGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                     <  1:2389280/43‑1
                                                        GGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         >  1:2510946/1‑70
                                                        GGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         >  1:101541/1‑70
                                                        GGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         >  1:5240250/1‑70
                                                        GGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         >  1:269705/1‑70
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         <  1:3880024/69‑1
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         <  1:5163296/69‑1
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC         <  1:4078524/69‑1
                                                           AACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATT     >  1:585117/1‑71
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  1:2236829/1‑71
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  1:2048973/1‑71
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  1:2681414/1‑71
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  1:5312669/1‑71
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  1:3552028/1‑71
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  1:3736994/1‑71
                                                                   AGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTG                     >  1:105936/1‑47
                                                                   AGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTG                     >  1:1311926/1‑47
                                                                   AGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTT                      >  1:739919/1‑46
                                                                                                                                     
TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3746840‑3746912
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA  >  W3110S.gb/3748112‑3748171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.