| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 153,700 | A→G | 5.9% | L576P (CTT→CCT) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 153,700 | 0 | A | G | 5.9% | 251.2 / 6.3 | 85 | L576P (CTT→CCT) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (42/38); new base G (2/3); total (44/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCT > W3110S.gb/153633‑153764 | aaTACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGa < 1:879964/70‑1 (MQ=255)aaTACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGa < 1:2856313/70‑1 (MQ=255)aaTACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGa < 1:3444460/70‑1 (MQ=255) ctGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAGGATAAACGc > 1:4566739/1‑71 (MQ=255) ctGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAGGATAAACGc > 1:139722/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGc < 1:2847915/68‑1 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGc < 1:3328586/68‑1 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGc < 1:1867200/68‑1 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:75992/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:2469796/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:5048092/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:1584684/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:4757365/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:711147/1‑71 (MQ=255) gAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAACGGTGAAACTAAGATAAACGCTAt > 1:202978/1‑71 (MQ=255) aaaCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATc < 1:222221/71‑1 (MQ=255) aaaCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATc < 1:1408326/71‑1 (MQ=255) aCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAGGATAAACGCTATCGt < 1:3887918/71‑1 (MQ=255) aCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAGGATAAACGCTATCGt < 1:3881221/71‑1 (MQ=255) aCCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAGGATAAACGCTATCGt < 1:1492145/71‑1 (MQ=255) gAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAgt > 1:356260/1‑71 (MQ=255) gAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAgt > 1:5147838/1‑71 (MQ=255) aaGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGAt > 1:957086/1‑54 (MQ=255) aaGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGAt > 1:4644040/1‑54 (MQ=255) gttCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCt > 1:5303661/1‑71 (MQ=255) gttCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCt > 1:3195847/1‑71 (MQ=255) gttCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCt > 1:246275/1‑71 (MQ=255) ttCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCt < 1:4887037/70‑1 (MQ=255) ttCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCt < 1:1866744/70‑1 (MQ=255) aGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCAt < 1:774049/71‑1 (MQ=255) aGTGCCAAGTAATTTTTCAATCGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCAt < 1:5130619/71‑1 (MQ=255) tGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTc < 1:4274537/71‑1 (MQ=255) gCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTc > 1:3587404/1‑70 (MQ=255) gCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTc > 1:1502793/1‑70 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:657878/1‑46 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:5285967/1‑46 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:2988434/1‑46 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:714421/1‑46 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTAt > 1:5201478/1‑46 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTc > 1:4400424/1‑65 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTc > 1:697352/1‑65 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTc > 1:3757545/1‑65 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTc > 1:690598/1‑65 (MQ=255) ccAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCa > 1:731165/1‑71 (MQ=255) aaGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCAt < 1:2594276/65‑1 (MQ=255) aaGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATg < 1:676950/71‑1 (MQ=255) aaGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATg < 1:3115544/71‑1 (MQ=255) aaGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATg < 1:851668/71‑1 (MQ=255) aaGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATg < 1:4799389/71‑1 (MQ=255) aGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGa < 1:4232504/71‑1 (MQ=255) aGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGa < 1:1130637/71‑1 (MQ=255) aGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGa < 1:3349848/71‑1 (MQ=255) tAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTc < 1:5251369/45‑1 (MQ=255) tAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTc < 1:4409139/45‑1 (MQ=255) aaTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTc < 1:1773543/44‑1 (MQ=255) aaTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACg > 1:4241193/1‑71 (MQ=255) aaTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACg > 1:1199993/1‑71 (MQ=255) aTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:2342771/1‑45 (MQ=255) aTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:4820960/1‑45 (MQ=255) aTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:5323076/1‑45 (MQ=255) aTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGc < 1:4886409/71‑1 (MQ=255) aTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGc < 1:2452926/71‑1 (MQ=255) aTTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGc < 1:2363743/71‑1 (MQ=255) tttCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTg < 1:526855/70‑1 (MQ=255) aTTGGAATGGTGAAACTAAGATAAACGCTATCGTc < 1:5577994/35‑1 (MQ=255) aTTGGAATGGTGAAACTAAGATAAACGCTATCGTc < 1:4454770/35‑1 (MQ=255) aTTGGAATGGTGAAACTAAGATAAACGCTATCGTc < 1:3922976/35‑1 (MQ=255) tGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:5182771/1‑35 (MQ=255) tGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:2842542/1‑35 (MQ=255) tGGAATGGTGAAACTAAGATAAACGCTATCGTCAg > 1:1051853/1‑35 (MQ=255) gAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGa < 1:5340263/69‑1 (MQ=255) tGGTGAAACTAAGATAAACGATATCGTCAGTGTCGCCGTCTTCATTCCATGAACg < 1:2244897/55‑1 (MQ=255) gTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGa < 1:5030863/64‑1 (MQ=255) gTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGa < 1:4534324/64‑1 (MQ=255) gTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGa < 1:3382808/64‑1 (MQ=255) gTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTg > 1:3630634/1‑69 (MQ=255) gTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTa < 1:1629395/71‑1 (MQ=255) gTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTa < 1:3241350/71‑1 (MQ=255) gAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGaca > 1:3087025/1‑64 (MQ=255) gAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGaca > 1:2594587/1‑64 (MQ=255) gAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGc > 1:122942/1‑71 (MQ=255) gAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGc > 1:5148981/1‑71 (MQ=255) gAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGc > 1:3961791/1‑71 (MQ=255) gAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGc > 1:3198038/1‑71 (MQ=255) aaaCTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCt > 1:1511179/1‑71 (MQ=255) | AATACTCTGGAAACCTGAAGTACGTTGTTCAGTGCCAAGTAATTTTTCAATTGGAATGGTGAAACTAAGATAAACGCTATCGTCAGTGTCGCCGTCTTCATTCCATGAACGCTGGGCACTGACACTGTAGCT > W3110S.gb/153633‑153764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |