| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 671,132 | C→T | 9.7% | R299H (CGT→CAT) | holA ← | DNA polymerase III, delta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 671,132 | 0 | C | T | 9.7% | 289.3 / 16.8 | 93 | R299H (CGT→CAT) | holA | DNA polymerase III, delta subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/50); new base T (4/5); total (38/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01 | |||||||||||
CCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCC > W3110S.gb/671064‑671198 | cccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:2292839/71‑1 (MQ=255)cccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:5119690/71‑1 (MQ=255)cccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:3331405/71‑1 (MQ=255)cccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:3366257/71‑1 (MQ=255)cccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:3578545/71‑1 (MQ=255)cccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:3595167/71‑1 (MQ=255) ccACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGt < 1:2437067/70‑1 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:724623/1‑71 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:2529986/1‑71 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:2646525/1‑71 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:2687347/1‑71 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:1995348/1‑71 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:1745781/1‑71 (MQ=255) acacTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAc > 1:4076636/1‑71 (MQ=255) actgactgACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACt > 1:5235341/1‑70 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCg > 1:2032286/1‑69 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGt > 1:3134803/1‑70 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGt > 1:1076179/1‑70 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGt > 1:2208361/1‑70 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTc > 1:4731983/1‑71 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTc > 1:5077401/1‑71 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTc > 1:4616054/1‑71 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTc > 1:1831829/1‑71 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTc > 1:3495839/1‑71 (MQ=255) actgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGACTGACGTAACTGCGTc > 1:1048012/1‑71 (MQ=255) tgacCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCt > 1:4811048/1‑70 (MQ=255) acCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGAc > 1:3793363/1‑71 (MQ=255) acCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGAc > 1:2181258/1‑71 (MQ=255) acCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAAATGCGTCTGAc > 1:2164055/1‑71 (MQ=255) ttGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAt > 1:1007750/1‑71 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:4329183/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:1644682/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:235965/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:2270666/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:2661982/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:1651494/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:2833064/70‑1 (MQ=255) tttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:3387281/70‑1 (MQ=255) ttGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:2364276/69‑1 (MQ=255) aGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACg < 1:3387509/70‑1 (MQ=255) aGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACg < 1:5379679/70‑1 (MQ=255) ggTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:5309051/64‑1 (MQ=255) ccGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGa > 1:4790842/1‑44 (MQ=255) ccGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGa > 1:5576730/1‑44 (MQ=255) ccGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcat > 1:3221896/1‑70 (MQ=255) tCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCca < 1:3377818/60‑1 (MQ=255) tCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCca < 1:3224855/60‑1 (MQ=255) tCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCca < 1:828333/60‑1 (MQ=255) tCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCca < 1:985287/60‑1 (MQ=255) aGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:65046/45‑1 (MQ=255) aGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGAttt < 1:734317/45‑1 (MQ=255) gAGTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGAttt < 1:1003541/43‑1 (MQ=255) gAGTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGAttt < 1:5346173/43‑1 (MQ=255) gAGTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGAttt < 1:3523723/43‑1 (MQ=255) gAGTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGAttt < 1:5061771/43‑1 (MQ=255) gTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGtttt < 1:5017883/41‑1 (MQ=38) gTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGATTTaa > 1:2157582/1‑43 (MQ=255) gTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGATTTaa > 1:5059191/1‑43 (MQ=255) gTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGATTTaa > 1:405024/1‑43 (MQ=255) gTTGCACGGCCTGATGTAACTGCGTCTGACTTAAGCGATTTaa > 1:3005599/1‑43 (MQ=255) ttGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:5280669/50‑1 (MQ=255) ttGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:2017861/50‑1 (MQ=255) ttGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:4532361/50‑1 (MQ=255) ttGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:1428357/50‑1 (MQ=255) ttGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:702585/50‑1 (MQ=255) ttGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:685039/50‑1 (MQ=255) tGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGc < 1:150592/49‑1 (MQ=255) gCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGc > 1:2474628/1‑71 (MQ=255) ctaaCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:1753276/68‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCt < 1:1437721/62‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCt < 1:3612110/62‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCt < 1:383907/62‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCt < 1:138717/62‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCt < 1:5432388/62‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCt < 1:4375500/62‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:2097588/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:3144268/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:772100/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:95804/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:3084606/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:219437/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:522890/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:1484870/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:1492946/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:4493354/71‑1 (MQ=255) cTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATAccc < 1:4124246/71‑1 (MQ=255) aCGTAACTGCGTCTGACTTAATCGATTTAACGCCTCGCCcatca > 1:2396561/1‑44 (MQ=255) aCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatca > 1:1366144/1‑44 (MQ=255) aCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatca > 1:1604260/1‑44 (MQ=255) aCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatca > 1:4783417/1‑44 (MQ=255) aCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatca > 1:322547/1‑44 (MQ=255) aCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatca > 1:3138433/1‑44 (MQ=255) aCGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCcatca > 1:87479/1‑44 (MQ=255) | CCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCC > W3110S.gb/671064‑671198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |