| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 711,258 | T→A | 11.7% | intergenic (‑190/+99) | fur ← / ← fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 711,258 | 0 | T | A | 11.7% | 257.7 / 23.8 | 94 | intergenic (‑190/+99) | fur/fldA | DNA‑binding transcriptional dual regulator/flavodoxin 1 |
| Reads supporting (aligned to +/- strand): ref base T (34/49); new base A (7/4); total (41/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
AAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATA > W3110S.gb/711190‑711325 | aaaTATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:4184720/71‑1 (MQ=255)aaaTATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:4317089/71‑1 (MQ=255)aaaTATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:4498650/71‑1 (MQ=255) atatTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTa < 1:1368439/71‑1 (MQ=255) ttGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTa > 1:3884160/1‑68 (MQ=255) ttGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTa > 1:3297084/1‑68 (MQ=255) ttGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTa > 1:4341769/1‑68 (MQ=255) ttGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTa > 1:2000678/1‑68 (MQ=255) ttGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTa > 1:4836106/1‑68 (MQ=255) aataaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTac < 1:3994131/70‑1 (MQ=255) aataaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTac < 1:1572130/70‑1 (MQ=255) ataaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTac < 1:2572018/69‑1 (MQ=255) taaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa < 1:1149516/70‑1 (MQ=255) taaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa < 1:3528954/70‑1 (MQ=255) taaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa < 1:4315501/70‑1 (MQ=255) taaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa < 1:2725383/70‑1 (MQ=255) taaAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa < 1:5378164/70‑1 (MQ=255) taaAAACGTGGCACAGCTTACA‑TTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa < 1:924395/69‑1 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa > 1:236121/1‑68 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa > 1:692210/1‑68 (MQ=255) aaaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTacaa > 1:295664/1‑68 (MQ=255) aaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAg > 1:3830079/1‑70 (MQ=255) aaaCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAg > 1:760127/1‑70 (MQ=255) aCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa < 1:5482477/71‑1 (MQ=255) aCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa < 1:1472123/71‑1 (MQ=255) aCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa < 1:5139644/71‑1 (MQ=255) aCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa < 1:4727347/71‑1 (MQ=255) aCGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa < 1:4774956/71‑1 (MQ=255) aCGTGGCAAAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa < 1:3286070/71‑1 (MQ=255) cGTGGCACAGCTTACATTTACAACGGTAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa > 1:4908574/1‑70 (MQ=255) cGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa > 1:1932490/1‑70 (MQ=255) cGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa > 1:5596218/1‑70 (MQ=255) cGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCaa > 1:5339302/1‑70 (MQ=255) aGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:4983198/1‑70 (MQ=255) aGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:4229059/1‑70 (MQ=255) aGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:979252/1‑70 (MQ=255) aGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:3736555/1‑70 (MQ=255) aGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACa > 1:2481526/1‑70 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:3928046/44‑1 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:4070649/44‑1 (MQ=255) gCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTg < 1:4557991/44‑1 (MQ=255) aCAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCaa > 1:482686/1‑71 (MQ=255) aCAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCa < 1:694639/70‑1 (MQ=255) aCAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCa < 1:1911335/70‑1 (MQ=255) ggCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAattat < 1:3571877/70‑1 (MQ=255) cAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTAtttt < 1:5280959/71‑1 (MQ=255) cAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTAtttt < 1:3219146/71‑1 (MQ=255) aaGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:3839149/71‑1 (MQ=255) aaGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:659963/71‑1 (MQ=255) aaGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:4484858/71‑1 (MQ=255) aaGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:3149791/71‑1 (MQ=255) aaGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:1685306/71‑1 (MQ=255) aaGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGAAGCAATTATTTTg < 1:2487198/71‑1 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAat < 1:316835/62‑1 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAat < 1:700203/62‑1 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa < 1:2378761/70‑1 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa < 1:3155109/70‑1 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa < 1:2355029/70‑1 (MQ=255) ggCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa < 1:625759/70‑1 (MQ=255) ccACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGa < 1:4548456/68‑1 (MQ=255) aCTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTgg > 1:1935389/1‑70 (MQ=255) aCTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTgg > 1:3817482/1‑70 (MQ=255) aCTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTgg > 1:918314/1‑70 (MQ=255) aCTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTgg > 1:4818108/1‑70 (MQ=255) aCTTGAACACCGGGA‑ATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCa > 1:1064471/1‑71 (MQ=255) ttGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:455759/63‑1 (MQ=255) ttGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt > 1:721986/1‑71 (MQ=255) ttGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt > 1:4097117/1‑71 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt > 1:3649912/1‑70 (MQ=255) tGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCAt > 1:3922649/1‑70 (MQ=255) gAACACCGGGACATTGTACAGGTACAACAGCAAAAGt > 1:3709991/1‑37 (MQ=255) gAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGAt > 1:3745024/1‑63 (MQ=255) gAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGAt > 1:923944/1‑63 (MQ=255) gAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGAt > 1:375850/1‑63 (MQ=255) gAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGAt > 1:2606219/1‑63 (MQ=255) acacCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATc < 1:4410617/71‑1 (MQ=255) acCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTa < 1:789155/71‑1 (MQ=255) ggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTg < 1:2866655/53‑1 (MQ=255) ggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTaa < 1:5016123/71‑1 (MQ=255) ggACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTaa < 1:5312979/71‑1 (MQ=255) taCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTaa < 1:56839/69‑1 (MQ=255) taCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTaa < 1:4349092/69‑1 (MQ=255) taCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTaa < 1:2040489/69‑1 (MQ=255) taCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTaa < 1:2566901/69‑1 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAAt > 1:5298537/1‑70 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa > 1:4785368/1‑71 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa > 1:1603550/1‑71 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa > 1:1495799/1‑71 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa > 1:81127/1‑71 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa > 1:1262089/1‑71 (MQ=255) aCAATGTCCCGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATa > 1:950280/1‑71 (MQ=255) aTTGTACAGGTACAACAGCGAAAGTTACAAATTTGTAGCAATTATTTTg < 1:2517312/49‑1 (MQ=255) aTTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTAtttt < 1:4586683/48‑1 (MQ=255) aTTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTAtttt < 1:4603933/48‑1 (MQ=255) | AAATATTGTTAATAAAAACGTGGCACAGCTTACATTTACAACGGCAAGGCCACTTGAACACCGGGACATTGTACAGGTACAACAGCAAAAGTTACAAATTTGTAGCAATTATTTTGATTGGCATTATCTATTAATA > W3110S.gb/711190‑711325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |