| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 54.3% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 54.3% | 20.7 / 137.0 | 94 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (21/22); new base T (23/28); total (44/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGG > W3110S.gb/70513‑70645 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTg > 1:3444258/1‑71 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTg > 1:3685659/1‑71 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTg > 1:183723/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGCCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt > 1:917202/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg < 1:5220072/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:5133304/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:2913998/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:4151004/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:4145618/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:1888754/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:850184/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:788885/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:331229/69‑1 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCgg < 1:5036914/57‑1 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGt < 1:2413237/71‑1 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGt < 1:1305949/71‑1 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCATGGACGAGAATTTGTCt > 1:5170805/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTc > 1:2218191/1‑71 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:572919/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:4367151/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:4939804/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:5512427/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:5085726/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:3655666/1‑45 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGt < 1:2015202/69‑1 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc > 1:1813637/1‑71 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc > 1:1369107/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:1196987/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:962495/1‑63 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:4743565/1‑63 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:4952500/1‑63 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTccc > 1:4991443/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATAAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:413142/1‑63 (MQ=255) cgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:5510844/71‑1 (MQ=255) cgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:4328748/71‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:1219187/71‑1 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:2336716/1‑70 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:3990786/1‑70 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:484923/71‑1 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:1012118/71‑1 (MQ=255) gggggTTGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg < 1:4224577/55‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:2113476/71‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:855894/71‑1 (MQ=255) ggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:4039658/1‑70 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtttt < 1:3405407/47‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtttt < 1:1156815/47‑1 (MQ=255) tgAAAAATCGGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:4278009/68‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCgg < 1:236808/36‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTTCTGTTCCCGcc < 1:1737020/58‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:5574617/58‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:695725/58‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:4090087/58‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1770351/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1988790/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:4534794/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:3037640/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:3935872/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:3326393/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:3168300/1‑70 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:4334203/1‑70 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:1807134/1‑70 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt < 1:2119996/47‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:3779843/57‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:5546807/57‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:3718198/1‑58 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:4791444/1‑58 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1007315/1‑58 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCAGGTGATATTTTGCTGTTCCCGCCAg > 1:1671166/1‑58 (MQ=39) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc > 1:5097668/1‑70 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:468447/35‑1 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:5369151/35‑1 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:3228862/35‑1 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCa < 1:3169883/56‑1 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:352394/1‑35 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:4405003/1‑35 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:3805558/1‑35 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:5599769/71‑1 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:559018/49‑1 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:2796488/49‑1 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:2766205/49‑1 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc > 1:2920505/1‑66 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcg > 1:1422538/1‑71 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:5464891/1‑71 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:1145479/1‑71 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:1291253/71‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc < 1:3086131/71‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc < 1:1897227/71‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc < 1:4819322/58‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc < 1:5380525/58‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc < 1:5096763/58‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc < 1:1803065/58‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc < 1:4792128/58‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc < 1:3425378/58‑1 (MQ=255) aTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCgg < 1:1045346/71‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1492543/44‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:464358/44‑1 (MQ=255) | AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGG > W3110S.gb/70513‑70645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |