| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,093,686 | T→C | 100% | V130A (GTA→GCA) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,093,686 | 0 | T | C | 100.0% | 251.2 / NA | 70 | V130A (GTA→GCA) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (39/31); total (39/31) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTG > W3110S.gb/1093621‑1093750 | ccAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg > 1:1243847/1‑70 (MQ=255) aaaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGa < 1:260456/71‑1 (MQ=255) aTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGaa > 1:908183/1‑70 (MQ=255) aTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGaa > 1:192139/1‑70 (MQ=255) ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:289283/71‑1 (MQ=255) ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:3709686/71‑1 (MQ=255) aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTATTATTTCTGGCATTGGTGAATAAAGTTAGTGa < 1:4378343/71‑1 (MQ=255) aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa < 1:2772180/71‑1 (MQ=255) aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa < 1:162722/71‑1 (MQ=255) aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa < 1:1358054/71‑1 (MQ=255) tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:3145062/1‑71 (MQ=255) tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaa > 1:5014498/1‑70 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:285133/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:3686390/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:2788822/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:2462517/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:2480796/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCCGGCATTGGTGAATAAAGTTAGTGaa < 1:5127494/67‑1 (MQ=255) attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAacac > 1:4641271/1‑70 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTTGTGAATAAAGTTAGTGAAAACACaa < 1:2696927/71‑1 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:1284947/71‑1 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:807149/71‑1 (MQ=255) ttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:1332002/70‑1 (MQ=255) gTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa < 1:1172957/70‑1 (MQ=255) tttCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa < 1:1241244/69‑1 (MQ=255) tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:221281/62‑1 (MQ=255) tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:2467625/62‑1 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:2837511/1‑62 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:994363/1‑62 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATaaa > 1:1299986/1‑35 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:2467101/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:1019049/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:574983/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:5025492/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:1397905/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:3746258/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:2425490/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:1988340/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:3045224/1‑36 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTc > 1:1728669/1‑70 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:2635292/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:5205156/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:799045/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:4832733/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:4102881/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:360758/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa > 1:1173831/1‑40 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaa < 1:3683057/71‑1 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaa < 1:3448316/71‑1 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaa < 1:4726385/71‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:3983421/36‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:3115942/36‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:3371203/1‑45 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaaa < 1:3272897/70‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:1404196/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:5329926/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:1911536/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:4511538/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:528044/1‑71 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:1297751/1‑71 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:5477546/1‑36 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:3281577/1‑36 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:4129913/1‑36 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:3639860/1‑36 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg > 1:4424576/1‑70 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACttt > 1:2286916/1‑71 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtg < 1:1281111/68‑1 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtg < 1:1265460/68‑1 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtg < 1:1827514/68‑1 (MQ=255) tGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtg < 1:3143721/68‑1 (MQ=255) | CCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTG > W3110S.gb/1093621‑1093750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |