| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 198.7 / NA | 63 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (40/23); total (40/23) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT > W3110S.gb/1303568‑1303695 | aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:4726848/1‑71 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:3666228/71‑1 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:3679657/71‑1 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:817153/71‑1 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:4214973/71‑1 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt > 1:975281/1‑70 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:3208250/71‑1 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa > 1:4973330/1‑71 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa > 1:5044570/1‑71 (MQ=255) cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt < 1:1698967/71‑1 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:5370393/1‑70 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:2834241/1‑70 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:77259/1‑70 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:1662691/1‑70 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:1395808/1‑70 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:1186365/1‑70 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa > 1:698988/1‑70 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:1007495/1‑71 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:2454497/71‑1 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:4425721/71‑1 (MQ=255) cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:5232662/71‑1 (MQ=255) cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2047429/71‑1 (MQ=255) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1166751/48‑1 (MQ=39) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:3185843/55‑1 (MQ=255) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:3806003/55‑1 (MQ=255) cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:850217/55‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:1399929/1‑55 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:5201062/1‑55 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:880406/1‑55 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:969172/1‑55 (MQ=255) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2810480/48‑1 (MQ=39) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:3782532/48‑1 (MQ=39) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:944541/48‑1 (MQ=39) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2088044/48‑1 (MQ=39) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:4362369/48‑1 (MQ=39) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2228725/48‑1 (MQ=39) ggAACAACGCGAAAACCGTTATTAACTATGTAACCt > 1:2041738/1‑36 (MQ=37) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc > 1:5148149/1‑71 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:837928/1‑45 (MQ=38) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:2136897/1‑45 (MQ=38) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:2361959/1‑45 (MQ=38) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:2415351/1‑45 (MQ=38) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:3161747/1‑45 (MQ=38) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:2650042/1‑45 (MQ=38) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:1892839/53‑1 (MQ=255) gcgAAAACCGTTATTAACTTTGTAACCTATTTGCCTATTGCTTCTGAAGTTACAGATGTCAACCGCTACCg > 1:3188639/1‑71 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg > 1:4269681/1‑71 (MQ=255) gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa < 1:3500912/71‑1 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:4233077/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:3727442/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:4153589/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:4232944/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:527846/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:4303629/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:2600478/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:4597010/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:4864545/1‑37 (MQ=37) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa < 1:573047/70‑1 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:4637617/1‑71 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:2140800/1‑71 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:285682/1‑71 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:4839239/1‑71 (MQ=255) attaACTATGTAACCTATTTGCCTAATGCTTCTGAAg > 1:4629413/1‑37 (MQ=25) | AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT > W3110S.gb/1303568‑1303695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |