Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,490,052 C→T 6.7% S36F (TCC→TTC)  aldA → aldehyde dehydrogenase A, NAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,490,0520CT 6.7% 345.7 / 11.1 104S36F (TCC→TTC) aldAaldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (49/48);  new base T (2/5);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 104 total aligned reads displayed.

TTTGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAG  >  W3110S.gb/1489988‑1490120
                                                                |                                                                    
tttGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCAt                                                                <  1:3711495/71‑1 (MQ=255)
tttGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCAt                                                                <  1:132103/71‑1 (MQ=255)
  tGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATa                                                               >  1:1488117/1‑70 (MQ=255)
  tGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAc                                                              >  1:4392874/1‑71 (MQ=255)
  tGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAc                                                              >  1:2207904/1‑71 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:832635/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:4016986/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:2785046/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:2774840/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:1998444/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:4903608/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:5050736/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:5058756/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:618044/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:1520312/71‑1 (MQ=255)
    ttACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:5252238/71‑1 (MQ=255)
     tACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATAccc                                                            <  1:4912732/70‑1 (MQ=255)
         tGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTTTCATTTTCCGCATACCCGATgg                                                       >  1:5398705/1‑71 (MQ=255)
         tGGCGTGGAGACGCATGGATTGATGTGGTAAAACCTGCTACAGAGGCTGTCATTTTCCGCATACACGATgg                                                       >  1:2288734/1‑71 (MQ=255)
           gCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGt                                                      >  1:4920426/1‑70 (MQ=255)
           gCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGAAGGt                                                      >  1:3552688/1‑70 (MQ=255)
                   aCGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:1670064/71‑1 (MQ=255)
                   aCGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:1661637/71‑1 (MQ=255)
                   aCGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:1820263/71‑1 (MQ=255)
                   aCGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:4711299/71‑1 (MQ=255)
                      cATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTTCCGCATACCCGATGGTCAGGCCGAGGAt                                          <  1:3690205/71‑1 (MQ=255)
                      cATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTTCCGCATACCCGATGGTCAGGCCGAGGAt                                          <  1:1874732/71‑1 (MQ=255)
                      cATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTTCCGCATACCCGATGGTCAGGCCGAGGAt                                          <  1:4909590/71‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCa                                                                 >  1:5425562/1‑46 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:4751987/66‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:5068406/66‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:3212603/66‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:2478610/66‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:390131/66‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:2968446/66‑1 (MQ=255)
                        tGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:2952657/66‑1 (MQ=255)
                                      aaaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATc                           >  1:1577235/1‑70 (MQ=255)
                                      aaaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATc                           >  1:3906076/1‑70 (MQ=255)
                                      aaaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATc                           >  1:4319950/1‑70 (MQ=255)
                                       accccTGCTACAGAGGCTGTCAGTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGa                         >  1:3872008/3‑71 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCTTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGa                         >  1:1930641/1‑71 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAg                                                   >  1:2516549/1‑45 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGAt                                          >  1:4976532/1‑54 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGAt                                          >  1:2556454/1‑54 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGAt                                          >  1:2344378/1‑54 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGAt                                          >  1:4783445/1‑54 (MQ=255)
                                       aaCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGa                         >  1:1289720/1‑71 (MQ=255)
                                        aCCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGcc                                                <  1:4464150/47‑1 (MQ=255)
                                         cccTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCATTCGATg                       <  1:721255/71‑1 (MQ=255)
                                         cccTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       <  1:413942/71‑1 (MQ=255)
                                         cccTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       <  1:1180773/71‑1 (MQ=255)
                                         cccTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       <  1:3192933/71‑1 (MQ=255)
                                         cccTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       <  1:2168948/71‑1 (MQ=255)
                                           cTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGAt                        >  1:883591/1‑68 (MQ=255)
                                           cTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       >  1:115190/1‑69 (MQ=255)
                                           cTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       >  1:1531569/1‑69 (MQ=255)
                                           cTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       >  1:1705268/1‑69 (MQ=255)
                                           cTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       >  1:2644550/1‑69 (MQ=255)
                                           cTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATg                       >  1:4068412/1‑69 (MQ=255)
                                              cTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:1907441/44‑1 (MQ=255)
                                              cTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:5260133/44‑1 (MQ=255)
                                                aCAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGa                                           >  1:1831111/1‑44 (MQ=255)
                                                aCAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGa                                           >  1:1832744/1‑44 (MQ=255)
                                                 cAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAg                                             <  1:3218664/41‑1 (MQ=255)
                                                    agGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGt            >  1:5357552/1‑71 (MQ=255)
                                                    agGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGt            >  1:3907751/1‑71 (MQ=255)
                                                       cTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGca         <  1:5320592/71‑1 (MQ=255)
                                                         gTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGcac        >  1:1252260/1‑70 (MQ=255)
                                                         gTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGcac        >  1:1861837/1‑70 (MQ=255)
                                                         gTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGcac        >  1:4927978/1‑70 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:4579064/1‑71 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:5403434/1‑71 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:1447114/1‑71 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:5087334/1‑71 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:662052/1‑71 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:3047870/1‑71 (MQ=255)
                                                           cATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:4202609/1‑71 (MQ=255)
                                                            aTTTTCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:5062633/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:1316414/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:382169/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:3187385/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:2950817/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:2646386/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:2137110/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAg                                 <  1:3048305/42‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGcagcag                 <  1:1670785/58‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGcagcag                 <  1:4766805/58‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGcagcag                 <  1:1710333/58‑1 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:4680390/1‑70 (MQ=255)
                                                            aTTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAAc     >  1:4444647/1‑70 (MQ=255)
                                                              ttCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGaa               >  1:1459368/1‑58 (MQ=255)
                                                              ttCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGaa               >  1:1214868/1‑58 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  >  1:3898408/1‑70 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  >  1:5285448/1‑70 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  >  1:4001219/1‑70 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  >  1:2343941/1‑70 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  <  1:4624332/70‑1 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  >  1:624593/1‑70 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  >  1:4400840/1‑70 (MQ=255)
                                                               tCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAg  <  1:3624981/70‑1 (MQ=255)
                                                                |                                                                    
TTTGTTACCTGGCGTGGAGACGCATGGATTGATGTGGTAAACCCTGCTACAGAGGCTGTCATTTCCCGCATACCCGATGGTCAGGCCGAGGATGCCCGTAAGGCAATCGATGCAGCAGAACGTGCACAACCAG  >  W3110S.gb/1489988‑1490120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: