| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,733,486 | C→A | 19.0% | L896M (CTG→ATG) | lhr → | predicted ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,733,486 | 0 | C | A | 19.0% | 188.0 / 21.1 | 63 | L896M (CTG→ATG) | lhr | predicted ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (36/15); new base A (5/7); total (41/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
GGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAT > W3110S.gb/1733417‑1733537 | ggCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGc < 1:1390510/70‑1 (MQ=255) tATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTgc > 1:5031359/1‑71 (MQ=255) cAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGtt < 1:1145424/59‑1 (MQ=255) cAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGtt < 1:1923669/59‑1 (MQ=255) cAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGtt < 1:17470/59‑1 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAg > 1:2504846/1‑52 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAg > 1:2397504/1‑52 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAg > 1:2368734/1‑52 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAg > 1:3527677/1‑52 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAg > 1:3647491/1‑52 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAg > 1:4012104/1‑52 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCg > 1:833278/1‑71 (MQ=255) cGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCg < 1:2970679/71‑1 (MQ=255) gCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCg > 1:3924016/1‑67 (MQ=255) gCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCg > 1:1007548/1‑67 (MQ=255) gCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCg > 1:1396037/1‑67 (MQ=255) gagCGCCGGGCATGCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:1716819/69‑1 (MQ=255) gagCGCCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:4104822/69‑1 (MQ=255) gagCGCCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:5504564/69‑1 (MQ=255) gagCGCCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:3175065/69‑1 (MQ=255) gagCGCCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:420452/69‑1 (MQ=255) gagCGCCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:5259002/69‑1 (MQ=255) gagCGCCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCg < 1:2486662/69‑1 (MQ=255) gcgcCGGGCATCTGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCggg > 1:3910613/1‑69 (MQ=255) gcgcCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCggg > 1:3124840/1‑69 (MQ=255) gcgcCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCggg > 1:390166/1‑69 (MQ=255) gcgcCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCggg > 1:2242094/1‑69 (MQ=255) gcgcCGGGCATCCGTACTGTCGATGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCgg > 1:621435/1‑68 (MQ=255) gcCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGaa > 1:2738063/1‑71 (MQ=255) gcCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGaa > 1:3920991/1‑71 (MQ=255) gcCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGaa > 1:1978025/1‑71 (MQ=255) gcCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGaa > 1:107785/1‑71 (MQ=255) aTCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCg < 1:1398678/53‑1 (MQ=255) aTCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCg < 1:4724928/53‑1 (MQ=255) aTCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCg < 1:747128/53‑1 (MQ=255) aTCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCg < 1:612708/53‑1 (MQ=255) aTCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCg < 1:2657894/53‑1 (MQ=255) aTCCGTACTGTCGCT‑‑ACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCg < 1:5165020/51‑1 (MQ=255) tCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAt < 1:477507/64‑1 (MQ=255) tCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAt < 1:5253284/64‑1 (MQ=255) tCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAt < 1:430690/64‑1 (MQ=255) tCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAt < 1:2562141/64‑1 (MQ=255) tCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAt < 1:3288485/64‑1 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCgg > 1:4535961/1‑43 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:1664774/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:4448957/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:4549293/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:5284266/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:811504/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:1940905/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:5011306/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:2015654/1‑53 (MQ=255) ccGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGAt > 1:57432/1‑53 (MQ=255) cGTACTGTGGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:1818581/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:3236465/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:4698154/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:3232880/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:3226249/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:296663/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:2480067/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:2346073/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:1842812/1‑53 (MQ=255) cGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATc > 1:1485733/1‑53 (MQ=255) cTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCgg > 1:911689/1‑52 (MQ=255) | GGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGTACTGTCGCTGGACAGCGAGTTACTGCGCAATCTACTCGGACAGGTCGATCCGGGGGAAT > W3110S.gb/1733417‑1733537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |