| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,227,651 | C→G | 21.1% | G36G (GGC→GGG) ‡ | ygjK → | predicted glycosyl hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,227,651 | 0 | C | G | 21.1% | 143.9 / 33.4 | 71 | G36G (GGC→GGG) ‡ | ygjK | predicted glycosyl hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (16/40); new base G (9/6); total (25/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.38e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGG > W3110S.gb/3227592‑3227719 | aGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTa > 1:3616519/1‑71 (MQ=255) ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcg < 1:3285005/68‑2 (MQ=37) ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcg < 1:5180493/68‑2 (MQ=37) ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcg < 1:1263489/68‑2 (MQ=37) ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcg < 1:4010414/68‑2 (MQ=37) ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcg < 1:1846295/68‑2 (MQ=37) ttCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcg < 1:1803750/68‑2 (MQ=37) ccGCCCATGCCGCTAACGCTGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:1262586/1‑65 (MQ=25) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGaa < 1:2621785/70‑1 (MQ=255) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:1857517/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:3191528/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:3174358/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:3332819/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:197431/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:4523540/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:1060512/1‑65 (MQ=37) ccGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGCGGCGCGCCAGTAcggt > 1:4926167/1‑65 (MQ=37) ccGCTAACGCCGACAATTATAAAAACGTTATTAACCGTACTGGCGCGCCGCAGTACATGAAGgattacgat > 1:4629915/1‑71 (MQ=255) ccGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGgattacgat > 1:5176388/1‑71 (MQ=255) cTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGgattacg < 1:1585350/66‑1 (MQ=255) cTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGgattacg < 1:3586652/66‑1 (MQ=255) cTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGgattacg < 1:1293413/66‑1 (MQ=255) aaCGCCGACAATTATAAAAACGTGATTAACCGTACTGgcg > 1:1370540/1‑40 (MQ=255) aaCGCCGACAATTATAAAAACGTGATTAACCGTACTGgcg > 1:1241596/1‑40 (MQ=255) aaCGCCGACAATTATAAAAACGTGATTAACCGTACTGgcg > 1:2075389/1‑40 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:2464103/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:3161597/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:330412/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:3590445/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:4253942/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:1707313/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:4595558/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGcgccgc < 1:1356880/35‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:656806/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:2467448/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:2515227/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:4725515/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:1997490/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:3287632/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:1016704/71‑1 (MQ=255) ttATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:1064458/71‑1 (MQ=255) ttATAAAAACCTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGc < 1:3244944/71‑1 (MQ=255) aaCGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa < 1:980321/71‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa < 1:2622447/64‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa < 1:2650900/64‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa < 1:1324293/64‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa < 1:2601004/64‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa < 1:4235892/64‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTa < 1:4697185/63‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTa < 1:2660527/63‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTa < 1:3116871/63‑1 (MQ=255) ttAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTa < 1:2566745/63‑1 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGTATTACGATTACGACGATCACCAGCGTTTTAATc > 1:3310775/1‑64 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTaa > 1:3909829/1‑62 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAAt > 1:1737061/1‑63 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAAt > 1:1364112/1‑63 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAAt > 1:1778352/1‑63 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAAt > 1:3970477/1‑63 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATc > 1:1979777/1‑64 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATc > 1:948254/1‑64 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATc > 1:1823851/1‑64 (MQ=255) aaCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATc > 1:1299525/1‑64 (MQ=255) aCCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTc < 1:1837735/71‑1 (MQ=255) ccGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCg < 1:5178824/71‑1 (MQ=255) cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAtctc < 1:2530456/71‑1 (MQ=255) cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAtctc < 1:3122429/71‑1 (MQ=255) cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAtctc < 1:1309012/71‑1 (MQ=255) cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAtctc < 1:834273/71‑1 (MQ=255) cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAtctc < 1:1171832/71‑1 (MQ=255) cTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATACGTTTTTCGAtctc < 1:589014/71‑1 (MQ=255) ggCGCGCCGCAGTACATGAAGGATTACGATTACGATGATCACCAGCGTTTTAATCCGTTTTTCGATCTCg > 1:4363482/1‑70 (MQ=255) ggCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGAtctc < 1:5575533/69‑1 (MQ=255) ggCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCg > 1:2767853/1‑70 (MQ=255) ggCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCg > 1:800835/1‑70 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:693678/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:1252220/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:1047218/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:664052/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:1054746/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:2333159/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:4462256/70‑1 (MQ=255) gcgcgcCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCgg < 1:1996065/70‑1 (MQ=255) | AGTTTTTCCGCCCATGCCGCTAACGCCGACAATTATAAAAACGTGATTAACCGTACTGGCGCGCCGCAGTACATGAAGGATTACGATTACGACGATCACCAGCGTTTTAATCCGTTTTTCGATCTCGG > W3110S.gb/3227592‑3227719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |