| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,514 | T→C | 100% | pseudogene (647/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,514 | 0 | T | C | 100.0% | 169.2 / NA | 48 | pseudogene (647/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/31); total (17/31) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑G‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAA > W3110S.gb/3824447‑3824579 | gACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCtt < 1:2313456/71‑1 (MQ=255) cGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttgt < 1:3025357/71‑1 (MQ=255) cGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttgt < 1:3996992/71‑1 (MQ=255) gggCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttgt < 1:3647412/70‑1 (MQ=255) cgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGc < 1:1428808/71‑1 (MQ=255) cgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGc < 1:4023225/71‑1 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCCCCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:3974297/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:3966724/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:1946438/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:303039/1‑71 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:1032396/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:3529524/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:3479879/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:3382178/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2927978/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2705091/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:2137022/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:1801507/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:1319736/70‑1 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:2542525/71‑1 (MQ=255) aGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:4534074/71‑1 (MQ=255) ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa < 1:4070384/71‑4 (MQ=255) ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa < 1:3374437/71‑4 (MQ=255) ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa < 1:1728560/71‑4 (MQ=255) ggggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa < 1:765373/71‑4 (MQ=255) ggggACCGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa < 1:2743841/71‑4 (MQ=255) gggACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g‑ggggaa < 1:2553953/70‑4 (MQ=255) gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGgaatcgaatc > 1:5599192/1‑71 (MQ=255) gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGgaatcgaatc > 1:928857/1‑71 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAg < 1:460388/70‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑GTGGGAATCGAATCCATTGTAGCGACGCAg < 1:4644712/70‑1 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggc < 1:81577/71‑1 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggc < 1:193377/71‑1 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggc < 1:2613572/71‑1 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCa > 1:3291855/1‑51 (MQ=255) gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggcgg > 1:826736/1‑70 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAggcggc > 1:4065948/1‑70 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg > 1:4750198/1‑71 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg > 1:4756516/1‑71 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg > 1:675291/1‑71 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg > 1:1481673/1‑71 (MQ=255) gCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTgg < 1:5503657/71‑1 (MQ=255) gCTACTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTgg < 1:3230419/71‑1 (MQ=255) aCTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa > 1:2565575/1‑70 (MQ=255) aCTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa > 1:2970863/1‑70 (MQ=255) aCTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa > 1:3451124/1‑70 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCaaa > 1:1117958/1‑71 (MQ=255) cTCACCTTGTTGGCGCGGGCCCGAGG‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCaaa < 1:2360676/71‑1 (MQ=255) | GACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑G‑GGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAA > W3110S.gb/3824447‑3824579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |