| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0% | 280.8 / ‑6.7 | 89 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (44/44); total (44/45) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA > W3110S.gb/4563522‑4563656 | ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:3558736/71‑1 (MQ=255)ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:3552419/71‑1 (MQ=255)ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:1433562/71‑1 (MQ=255)ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2615811/71‑1 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttac > 1:4212697/1‑67 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1095707/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:2715281/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1364394/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:5099935/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:5522803/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTAATAAATAGGCTGTAACGCCATTTTAc > 1:5223478/1‑70 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCACGTCATAAATAGGCTGTAACGCCATTTTACt > 1:414580/1‑71 (MQ=255) cagcagCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTg > 1:184843/1‑70 (MQ=255) cagcagCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt > 1:2492182/1‑71 (MQ=255) aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:4658413/69‑1 (MQ=255) aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:5233849/69‑1 (MQ=255) aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:5541080/69‑1 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:788892/1‑71 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:2323493/70‑1 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:3323283/70‑1 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:453330/70‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGt < 1:3487645/71‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:2895973/71‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:4927649/71‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:2353966/71‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:1551931/71‑1 (MQ=255) caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3464626/70‑1 (MQ=255) tCGCAGTTTTCCATCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:2546562/1‑70 (MQ=255) tCGCAGTTTTCCAGCAGGTCCTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:5574332/1‑70 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt > 1:3955593/1‑44 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:4800458/1‑45 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:4469845/1‑45 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:1174864/1‑45 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:5545122/1‑45 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTggga > 1:4935083/1‑60 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:5026259/68‑1 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:2334453/68‑1 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:5316252/68‑1 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3117238/68‑1 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3585724/68‑1 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:246594/1‑70 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:1101126/1‑70 (MQ=255) tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTAGCCCATGTGGAACACGGt < 1:3663155/68‑1 (MQ=255) gCATTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:171388/71‑1 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:5599455/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:4088512/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:3737380/71‑1 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:325581/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:2047465/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1335385/71‑1 (MQ=255) cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:3995016/70‑1 (MQ=255) ttttCCATCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:1801428/58‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:4872181/58‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:886903/71‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:4415821/71‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:4239734/71‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:5508675/71‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:1825407/71‑1 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:568864/1‑71 (MQ=255) gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:5065205/64‑1 (MQ=255) cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:5305425/1‑39 (MQ=255) cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgc > 1:5369627/1‑71 (MQ=255) cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgc > 1:4388021/1‑71 (MQ=255) cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgc > 1:4944137/1‑71 (MQ=255) ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:4257529/1‑70 (MQ=255) ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:5295151/1‑70 (MQ=255) ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:1176571/1‑70 (MQ=255) ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:2049799/1‑70 (MQ=255) gTCTTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3738253/51‑1 (MQ=255) tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc < 1:1958343/55‑1 (MQ=255) cATAAATAGGCTGTAACTCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:1092074/1‑70 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg > 1:1131356/1‑50 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:1166477/1‑51 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:5258548/1‑51 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:3723452/1‑51 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:4406952/1‑51 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa > 1:397967/1‑51 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:2413680/1‑70 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:4516004/1‑70 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg < 1:3011330/57‑1 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:392235/71‑1 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:5318806/71‑1 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:3708089/71‑1 (MQ=255) tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:5303384/47‑1 (MQ=255) tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:491030/47‑1 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc > 1:1928184/1‑71 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:2528427/1‑60 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:3651184/1‑71 (MQ=255) cGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg < 1:3578437/71‑1 (MQ=255) cGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCggg < 1:3131639/71‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa < 1:4019219/71‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa < 1:3602130/71‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa < 1:4555616/71‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa < 1:2885332/71‑1 (MQ=255) gCCATTTTACTGGCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGa < 1:5487294/71‑1 (MQ=255) | GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGGGA > W3110S.gb/4563522‑4563656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |