| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,637,896 | G→T | 7.1% | intergenic (+128/+19) | trpR → / ← yjjX | DNA‑binding transcriptional repressor, tryptophan‑binding/thiamin metabolism associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,637,896 | 0 | G | T | 7.1% | 281.2 / 5.1 | 84 | intergenic (+128/+19) | trpR/yjjX | DNA‑binding transcriptional repressor, tryptophan‑binding/thiamin metabolism associated protein |
| Reads supporting (aligned to +/- strand): ref base G (36/41); new base T (3/3); total (39/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGA > W3110S.gb/4637837‑4637966 | cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTTGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:3932512/1‑71 (MQ=255)cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCa > 1:2142232/1‑70 (MQ=255)cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:4126089/1‑71 (MQ=255)cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:3385638/1‑71 (MQ=255)cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:3295244/1‑71 (MQ=255)cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:1250181/1‑71 (MQ=255)cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:941589/1‑71 (MQ=255) tATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:1088812/1‑69 (MQ=255) tATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCAt > 1:4964050/1‑69 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCa > 1:4338640/1‑60 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCa > 1:908703/1‑60 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCa > 1:979880/1‑60 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCa > 1:1123324/1‑60 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCa > 1:1116925/1‑60 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATa > 1:4133783/1‑71 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATa > 1:4916570/1‑71 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATa > 1:1313281/1‑71 (MQ=255) cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATa > 1:3603537/1‑71 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGTCAGCGTCGCATc < 1:3739118/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGTCAGCGTCGCATc < 1:911906/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:141011/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:1540162/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:4308091/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:4798191/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:4642774/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:4301500/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:1688010/49‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa < 1:4846031/71‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa < 1:1369805/71‑1 (MQ=255) tAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa < 1:1028218/71‑1 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGTCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt > 1:3604587/1‑71 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGTCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt > 1:1263428/1‑71 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCTGGCGCTTAATACACGGCATTa < 1:2954500/70‑1 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa > 1:5474474/1‑70 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa > 1:5480988/1‑70 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa > 1:2489964/1‑70 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa < 1:2035167/70‑1 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTa < 1:1998194/70‑1 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt > 1:4230155/1‑71 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt > 1:5448123/1‑71 (MQ=255) aaTTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt > 1:733215/1‑71 (MQ=255) tttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:518799/46‑1 (MQ=255) tttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATc < 1:3578983/46‑1 (MQ=255) tttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:4615470/71‑1 (MQ=255) tttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:1866260/71‑1 (MQ=255) ttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:5569514/70‑1 (MQ=255) tCAATATGTTTGGTAGGCATGATAAGACGCGTCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa > 1:4866847/1‑71 (MQ=255) tCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCg > 1:3655033/1‑40 (MQ=255) tCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa > 1:1010131/1‑71 (MQ=255) tCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa > 1:3555600/1‑71 (MQ=255) tCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa > 1:3014703/1‑71 (MQ=255) tCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa > 1:2538582/1‑71 (MQ=255) aaTATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACg < 1:1029317/71‑1 (MQ=255) atatGTTTGGTAGGCATGATAAGACGCGTCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACg < 1:4955737/70‑1 (MQ=255) atatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACg < 1:939509/70‑1 (MQ=255) atatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACg < 1:4557652/70‑1 (MQ=255) tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGaaa > 1:3680868/1‑67 (MQ=255) atGTTTGGTAGGCATTATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACg < 1:5040222/68‑1 (MQ=255) tGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACGCGGCATTATGAAACGGACt > 1:1028213/1‑71 (MQ=255) tGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGAc > 1:4296512/1‑70 (MQ=255) tGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACt > 1:2099806/1‑71 (MQ=255) tGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACt > 1:4762897/1‑71 (MQ=255) tGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACt > 1:527140/1‑71 (MQ=255) tGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACt > 1:321680/1‑71 (MQ=255) tAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:4491237/71‑1 (MQ=255) tAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:1553669/71‑1 (MQ=255) tAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:291909/71‑1 (MQ=255) tAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:3130565/71‑1 (MQ=255) tAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:5313024/71‑1 (MQ=255) gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAgg < 1:870514/71‑1 (MQ=255) cATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGa < 1:3298368/71‑1 (MQ=255) cATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGa < 1:863626/71‑1 (MQ=255) cATGATAAGACGCGCCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGa < 1:5163491/71‑1 (MQ=255) tGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:3312516/50‑1 (MQ=255) tGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:4493466/50‑1 (MQ=255) tGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:4128721/50‑1 (MQ=255) tGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:4127715/65‑1 (MQ=255) tGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc < 1:79278/65‑1 (MQ=255) gATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:3856104/49‑1 (MQ=255) gATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:4446646/49‑1 (MQ=255) gATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:1914211/49‑1 (MQ=255) gATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGa < 1:5099322/49‑1 (MQ=255) cgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGt < 1:4408627/71‑1 (MQ=255) cgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGt < 1:4265444/71‑1 (MQ=255) ggCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGt > 1:30199/1‑70 (MQ=255) ggCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGt > 1:3111371/1‑70 (MQ=255) ggCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGt > 1:3907070/1‑70 (MQ=255) ggCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTg < 1:2068910/71‑1 (MQ=255) gCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGa < 1:3053162/71‑1 (MQ=255) gCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGa < 1:4813983/71‑1 (MQ=255) gCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGa < 1:21017/71‑1 (MQ=255) gCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGa < 1:3357204/71‑1 (MQ=255) | CTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGA > W3110S.gb/4637837‑4637966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |