| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,085 | T→C | 52.1% | S233S (AGT→AGC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,085 | 0 | T | C | 52.1% | 18.5 / 219.8 | 146 | S233S (AGT→AGC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (17/53); new base C (37/39); total (54/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 146 total aligned reads displayed.
TCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGA > W3110S.gb/71016‑71153 | tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc < 1:5502604/71‑1 (MQ=255) tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg > 1:5351748/1‑71 (MQ=255) tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg > 1:2722899/1‑71 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGa < 1:4317175/71‑1 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg > 1:4045101/1‑69 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGaa < 1:1859939/71‑1 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGaa < 1:3133395/71‑1 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCCTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg > 1:3445521/1‑69 (MQ=255) gCCAGCAGTTGGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg < 1:502071/71‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg < 1:4757975/71‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg < 1:4830536/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:2683505/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:1485998/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:232621/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:4012744/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:2929608/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:4336663/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:2160577/71‑1 (MQ=255) agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGc < 1:151106/71‑1 (MQ=255) agcagTTAGGGATTAGCGTCTCAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGc < 1:3198426/71‑1 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc < 1:3705886/71‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc > 1:4019099/1‑70 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc > 1:1434517/1‑70 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc > 1:2377764/1‑71 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:2255769/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1409563/70‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1631658/68‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1389919/68‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGAt < 1:4768192/71‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:765535/1‑70 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGTACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg < 1:3619978/71‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGATCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg < 1:5341382/71‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg < 1:1302002/71‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:4609424/55‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:2236537/55‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:5196111/55‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:3580640/55‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:2981686/55‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:642465/55‑1 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATg < 1:1120195/70‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:3797624/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:979264/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:3453518/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:475563/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:51562/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:4820662/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:1955626/42‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg < 1:2948139/42‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGCCCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGc > 1:5365748/1‑56 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGcc < 1:2050880/70‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGcc < 1:4667161/70‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1043298/62‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:4443491/62‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:3977443/62‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:3876295/62‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTa > 1:1840302/1‑70 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTAt < 1:791872/71‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTAt < 1:4842741/71‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGa > 1:17212/1‑62 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCg > 1:2568563/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAg > 1:5462929/1‑39 (MQ=37) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGc > 1:2931607/1‑63 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:3788002/1‑67 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:2408721/1‑67 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAG‑‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:1516647/1‑65 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:5369491/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:3557954/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:2852854/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:3645077/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc < 1:4616512/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc < 1:1855257/70‑1 (MQ=255) aGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGc < 1:4654725/48‑1 (MQ=255) cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg < 1:2319582/71‑1 (MQ=255) cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg < 1:1840284/71‑1 (MQ=255) gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggt > 1:1141106/1‑71 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:2886287/71‑1 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:1350411/71‑1 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:3847183/71‑1 (MQ=255) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:4922422/41‑1 (MQ=25) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:919584/41‑1 (MQ=25) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:3856863/41‑1 (MQ=25) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1471762/41‑1 (MQ=25) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:566379/41‑1 (MQ=25) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:256529/41‑1 (MQ=25) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:4221638/41‑1 (MQ=25) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:2160397/71‑1 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:3347281/1‑48 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:2192754/1‑48 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:4361877/1‑48 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:5456106/1‑48 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4331796/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:598685/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:5385352/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:3303891/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:3131458/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:339530/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:1267073/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:161460/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:3043487/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:5109999/1‑71 (MQ=255) | TCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGA > W3110S.gb/71016‑71153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |