Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb547,8311.G100.0% 73.7 / NA 23pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE
ylbE
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/8);  total (15/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC  >  W3110S.gb/547764‑547896
                                                                    |                                                                 
tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                    <  1:1492473/68‑1 (MQ=255)
tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                    <  1:1658587/68‑1 (MQ=255)
  cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAggggg                                                              <  1:2175880/71‑2 (MQ=255)
  cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgggg                                                               >  1:840390/1‑70 (MQ=255)
            tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg                                                          <  1:2060987/66‑1 (MQ=255)
                  ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAAc                                                       >  1:373133/1‑63 (MQ=255)
                  ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAt                                                  <  1:210861/68‑1 (MQ=255)
                    cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                             >  1:1099213/1‑71 (MQ=255)
                           tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGt                                           >  1:59747/1‑66 (MQ=255)
                           tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                      >  1:1212920/1‑71 (MQ=255)
                                gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg                                                          <  1:945814/46‑1 (MQ=38)
                                 aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                >  1:1331197/1‑71 (MQ=255)
                                  aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                               >  1:311544/1‑71 (MQ=255)
                                   aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                >  1:604155/1‑69 (MQ=255)
                                          gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                       >  1:1725689/1‑71 (MQ=255)
                                           cgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt                                     <  1:304632/56‑1 (MQ=255)
                                                          tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc                                       <  1:1068042/39‑1 (MQ=38)
                                                          tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtg                                    <  1:835405/42‑1 (MQ=38)
                                                          tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt        >  1:1098264/1‑70 (MQ=255)
                                                            cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt                                     >  1:1219036/1‑39 (MQ=38)
                                                            cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc                             >  1:1152485/1‑47 (MQ=255)
                                                            cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                     >  1:1624559/1‑55 (MQ=255)
                                                            cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                     >  1:1450696/1‑55 (MQ=255)
                                                            cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                  >  1:1607619/1‑58 (MQ=255)
                                                             tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc     <  1:1242600/70‑1 (MQ=255)
                                                              ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa         >  1:732643/1‑65 (MQ=255)
                                                               gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc  <  1:649360/71‑1 (MQ=255)
                                                                    |                                                                 
TGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC  >  W3110S.gb/547764‑547896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: