Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 547,831 | 1 | . | G | 100.0% | 73.7 / NA | 23 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (15/8); total (15/8) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC > W3110S.gb/547764‑547896 | tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:1492473/68‑1 (MQ=255) tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:1658587/68‑1 (MQ=255) cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggggg < 1:2175880/71‑2 (MQ=255) cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggg > 1:840390/1‑70 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg < 1:2060987/66‑1 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAAc > 1:373133/1‑63 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAt < 1:210861/68‑1 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt > 1:1099213/1‑71 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGt > 1:59747/1‑66 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa > 1:1212920/1‑71 (MQ=255) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg < 1:945814/46‑1 (MQ=38) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1331197/1‑71 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:311544/1‑71 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:604155/1‑69 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg > 1:1725689/1‑71 (MQ=255) cgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt < 1:304632/56‑1 (MQ=255) tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc < 1:1068042/39‑1 (MQ=38) tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtg < 1:835405/42‑1 (MQ=38) tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt > 1:1098264/1‑70 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt > 1:1219036/1‑39 (MQ=38) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc > 1:1152485/1‑47 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:1624559/1‑55 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc > 1:1450696/1‑55 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:1607619/1‑58 (MQ=255) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:1242600/70‑1 (MQ=255) ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa > 1:732643/1‑65 (MQ=255) gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc < 1:649360/71‑1 (MQ=255) | TGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC > W3110S.gb/547764‑547896 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |