Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262332 3262332 1–1199 42 [0] [0] 39 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)30 (1.440) 23/90 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAC  >  W3110S.gb/3262333‑3262390
                                                                                                                                    
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                               >  1:1650392/1‑71
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                               >  1:1481046/1‑71
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                               >  1:1555790/1‑71
  TATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTA                                                             <  1:1312881/71‑1
    TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                           >  1:2112696/1‑71
      TACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTT                                                         >  1:1695756/1‑71
        CCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                        <  1:1582516/70‑1
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                            >  1:1611574/1‑62
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                            >  1:1570159/1‑62
            CATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                            >  1:1567579/1‑62
             ATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                   <  1:931911/70‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                               <  1:2065244/71‑1
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                >  1:673029/1‑70
                AAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                >  1:670407/1‑70
                 AAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATG                                               >  1:2133728/1‑70
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                             <  1:147310/71‑1
                  AAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                             <  1:1089586/71‑1
                    ATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTA                                                   <  1:1972461/63‑1
                        TCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                <  1:883/62‑1
                            TCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATC                                      <  1:384731/68‑1
                                CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                >  1:1570533/1‑70
                                CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGC                                <  1:694356/70‑1
                                  ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAAT                             >  1:407367/1‑71
                                  ATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAA                              >  1:236211/1‑70
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATC                            <  1:1310263/71‑1
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA                                                            >  1:1820987/1‑39
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                              <  1:968050/37‑1
                                   TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTT                                                              <  1:1546445/37‑1
                                    ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                           >  1:7405/1‑71
                                         AACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                       <  1:1003725/70‑1
                                           CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                    <  1:540203/71‑1
                                           CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                    >  1:1096580/1‑71
                                              AATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTC                 >  1:992720/1‑71
                                                TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGT               <  1:2146674/71‑1
                                                   TGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA             <  1:548882/70‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGG        <  1:621222/71‑1
                                                       AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                          <  1:829144/53‑1
                                                        ATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTT                  <  1:959683/60‑1
                                                          TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAA      >  1:1861506/1‑70
                                                          TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAA      >  1:1166893/1‑70
                                                          TCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCG                >  1:1365248/1‑60
                                                           CTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT     <  1:1099828/70‑1
                                                                GGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                       <  1:1073938/47‑1
                                                                 GCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                       <  1:692327/46‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTT                       <  1:1668382/41‑1
                                                                      TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCAT                          <  1:619981/38‑1
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAC  >  1:123009/1‑60
                                                                        AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA             >  1:1278210/1‑49
                                                                                                                                    
AATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261060‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAAC  >  W3110S.gb/3262333‑3262390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.