| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,093,686 | T→C | 100% | V130A (GTA→GCA) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,093,686 | 0 | T | C | 100.0% | 117.3 / NA | 35 | V130A (GTA→GCA) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/15); total (20/15) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTT > W3110S.gb/1093624‑1093749 | aaaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTg > 1:2035765/1‑70 (MQ=255)aaaTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTg > 1:1980992/1‑70 (MQ=255) tGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa < 1:1619491/71‑1 (MQ=255) aCATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTgg > 1:2098006/1‑63 (MQ=255) aTTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt > 1:676915/1‑71 (MQ=255) aTTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt < 1:1712384/71‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg > 1:177203/1‑71 (MQ=255) cAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa < 1:1303084/63‑1 (MQ=255) cTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:990823/64‑1 (MQ=255) tattatTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt < 1:1609458/50‑1 (MQ=255) attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:1576249/1‑57 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:874401/71‑1 (MQ=255) tttGTTACGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:2049502/1‑66 (MQ=255) ttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:1876754/63‑1 (MQ=255) tCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAAGACaaaa > 1:871195/1‑67 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgcgc < 1:993497/71‑1 (MQ=255) cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:1743668/61‑1 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAat > 1:466754/1‑71 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta < 1:366852/71‑1 (MQ=255) gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:296427/1‑51 (MQ=255) gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:1308679/1‑51 (MQ=255) aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt < 1:1296873/71‑1 (MQ=255) aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt < 1:1304524/71‑1 (MQ=255) gTTTGTGCATATTAATATTTCTGGCATTGGTGAATa < 1:1975429/36‑1 (MQ=255) tttGTGCATATTAATATTTCTGGCATTGGTGAATaa < 1:1809081/36‑1 (MQ=255) gtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAAAAGCGCAATTTATTTTcc > 1:374121/1‑70 (MQ=255) tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:565001/38‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAAc > 1:1940006/1‑46 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:654572/1‑71 (MQ=255) tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca > 1:779731/1‑39 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:1043635/1‑43 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGa > 1:1768800/1‑71 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta > 1:1628392/1‑51 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta > 1:977280/1‑51 (MQ=255) tttCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACttt > 1:765906/1‑71 (MQ=255) | AAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTT > W3110S.gb/1093624‑1093749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |