| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,809,581 | G→A | 59.0% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,809,581 | 0 | G | A | 59.0% | 46.2 / 105.6 | 83 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (20/14); new base A (24/25); total (44/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTC > W3110S.gb/2809517‑2809649 | gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc < 1:1155367/71‑1 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:2013385/1‑70 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACg < 1:1695458/71‑1 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:2021721/1‑68 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:2164260/1‑68 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGAc > 1:410619/1‑71 (MQ=255) gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:796215/67‑1 (MQ=255) catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt < 1:1974532/71‑1 (MQ=255) catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt < 1:479163/71‑1 (MQ=255) tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:766515/59‑1 (MQ=255) tgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1748551/59‑1 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:1699801/1‑71 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:642877/1‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa > 1:316028/1‑55 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt < 1:1697555/70‑1 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt < 1:501294/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa > 1:492732/1‑56 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATg > 1:1207114/1‑71 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:316551/53‑1 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:104890/53‑1 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc < 1:809667/55‑1 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATgg > 1:2117259/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc < 1:144885/71‑1 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1448758/51‑1 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1192335/1‑67 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTaa > 1:937619/1‑50 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt > 1:265030/1‑62 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:1534007/71‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt > 1:2020618/1‑70 (MQ=255) caagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa > 1:1000592/1‑44 (MQ=255) caagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa < 1:1221092/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc < 1:1188160/48‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1468833/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1471491/71‑1 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:1530330/1‑69 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTaa < 1:1088943/45‑1 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTAc > 1:1141243/1‑71 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt < 1:247349/38‑1 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:894498/1‑70 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgt < 1:1451861/50‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgt > 1:1716407/1‑49 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:870565/1‑61 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg > 1:834274/1‑55 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:588319/71‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtctc < 1:1092594/71‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGAAATAACGAGACGTTGTTTATGGCGTTGATTa > 1:1436239/1‑59 (MQ=255) cGCACTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGt < 1:1850977/67‑1 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGt < 1:1973258/67‑1 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATg > 1:1882941/1‑47 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:445763/1‑54 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:1315818/1‑54 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:376316/1‑71 (MQ=255) gCAATAAAAAGCTGAAGGCTCAGGGGATTAACGAGACgt > 1:150417/1‑39 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa > 1:1518495/1‑63 (MQ=255) aTAAAATGCTTAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:1195782/71‑1 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTAt < 1:1167331/43‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:637092/40‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:1410285/70‑1 (MQ=255) aaaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:1718333/1‑71 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACgttgtt > 1:1260354/1‑38 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1032040/1‑40 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:720091/1‑37 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:1454587/1‑48 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTgg > 1:268077/1‑57 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1776450/1‑39 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:1591133/1‑51 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:1931023/1‑51 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa > 1:669306/1‑58 (MQ=255) aaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAcc < 1:2085363/71‑1 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg > 1:558489/1‑42 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:147827/71‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg > 1:1937196/1‑71 (MQ=255) tGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:820888/43‑1 (MQ=255) gAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:1207498/55‑1 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:2220282/1‑40 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:1022936/54‑1 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcac > 1:1255061/1‑66 (MQ=255) gCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg < 1:810839/42‑1 (MQ=255) gCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:2093978/71‑1 (MQ=255) gCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:917400/71‑1 (MQ=255) aGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:1910472/1‑36 (MQ=255) aGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:1527275/61‑1 (MQ=255) aGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCt > 1:1719978/1‑71 (MQ=255) gggATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTc > 1:2069844/1‑71 (MQ=255) | GTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTC > W3110S.gb/2809517‑2809649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |