Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3680GA100.0% 96.8 / NA 33pseudogene (501/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/26);  total (7/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGT  >  W3110S.gb/3824304‑3824433
                                                                |                                                                 
aaaaCGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACggt                                                             <  1:1499352/71‑2 (MQ=255)
    cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg                                                         >  1:1081814/1‑71 (MQ=255)
       cAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                      <  1:2038869/71‑3 (MQ=255)
           cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTaaa                                                                 >  1:1405512/1‑56 (MQ=255)
           cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                  <  1:616804/71‑1 (MQ=255)
            tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                  <  1:961848/70‑1 (MQ=255)
            tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                  <  1:1046261/70‑1 (MQ=255)
              aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACgg                                                              >  1:1190845/1‑56 (MQ=255)
                aaccaaccCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATc                                             <  1:1924906/71‑1 (MQ=255)
                      cccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                       <  1:2042826/54‑2 (MQ=255)
                      cccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                        <  1:831918/69‑1 (MQ=255)
                           tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGa                                                           <  1:1575113/46‑1 (MQ=255)
                           tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                  >  1:466770/1‑71 (MQ=255)
                             aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:382458/70‑1 (MQ=255)
                             aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:1139789/70‑1 (MQ=255)
                               aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                       <  1:395934/46‑2 (MQ=255)
                                  gATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAct                           >  1:1973572/1‑71 (MQ=255)
                                   aTGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActc                          <  1:2066570/71‑1 (MQ=255)
                                         gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                     >  1:1493688/1‑70 (MQ=255)
                                         gCCGCAGTTTCTGACGGTATTGTAAACGGTGAAGca                                                       <  1:1599791/36‑2 (MQ=16)
                                          ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGGGATCTGGAATACGTTGAAGACTCAGCCGCa                   <  1:564530/71‑1 (MQ=255)
                                          ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                   <  1:1524158/71‑1 (MQ=255)
                                              aGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                   <  1:528276/67‑1 (MQ=255)
                                               gTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAc              <  1:1811802/71‑1 (MQ=255)
                                                tttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                 <  1:894375/51‑1 (MQ=255)
                                                  tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                        >  1:394915/1‑42 (MQ=19)
                                                      tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGAc          <  1:361856/68‑1 (MQ=255)
                                                      tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGAc          <  1:1762627/68‑1 (MQ=255)
                                                          taTTGTAAACGGTAAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg   >  1:106682/2‑71 (MQ=255)
                                                            ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                               <  1:443284/41‑1 (MQ=25)
                                                            ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                               <  1:2122885/41‑1 (MQ=25)
                                                            ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                               <  1:183882/41‑1 (MQ=25)
                                                            ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                               <  1:1632251/41‑1 (MQ=25)
                                                            ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGt  <  1:2214843/70‑1 (MQ=255)
                                                                |                                                                 
AAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGT  >  W3110S.gb/3824304‑3824433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: