BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	80.1 / NA	28	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (14/14); total (14/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAC  >  W3110S.gb/4563523‑4563650

gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                           <  1:1398446/71‑1 (MQ=255)

           cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                <  1:1592046/71‑1 (MQ=255)

            ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                               >  1:2159106/1‑71 (MQ=255)

             tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                              >  1:1009356/1‑71 (MQ=255)

               cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg                                            <  1:292172/71‑1 (MQ=255)

                tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                            <  1:2181490/54‑1 (MQ=255)

                  aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                         >  1:1358074/1‑55 (MQ=255)

                  aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc                                                         >  1:295679/1‑55 (MQ=255)

                  aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                           <  1:1258928/69‑1 (MQ=255)

                  aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                           <  1:1473166/69‑1 (MQ=255)

                   tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                              <  1:2095759/65‑1 (MQ=255)

                        acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                   >  1:1340318/1‑71 (MQ=255)

                        acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt                                   <  1:1745891/71‑1 (MQ=255)

                            cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                              >  1:955626/1‑56 (MQ=255)

                             gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                          >  1:1797323/1‑43 (MQ=255)

                                 ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                           <  1:1224583/38‑1 (MQ=255)

                                   ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                        >  1:1503427/1‑71 (MQ=255)

                                    tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                        <  1:2161502/70‑1 (MQ=255)

                                     ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                       <  1:2010243/70‑1 (MQ=255)

                                        gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg                   >  1:156979/1‑71 (MQ=255)

                                            gTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                  <  1:1664872/36‑1 (MQ=255)

                                             tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg              >  1:1355042/1‑71 (MQ=255)

                                              cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg              >  1:1919725/1‑70 (MQ=255)

                                                tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                       <  1:1185651/43‑1 (MQ=255)

                                                tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                             <  1:1751733/53‑1 (MQ=255)

                                                tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                             <  1:1754139/53‑1 (MQ=255)

                                                 aaaTAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                              >  1:336256/1‑35 (MQ=255)

                                                 aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg                                           >  1:354898/1‑38 (MQ=255)

                                                  aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa          <  1:1645787/70‑1 (MQ=255)

                                                      ggCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                         >  1:1982172/1‑35 (MQ=255)

                                                           tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc  <  1:999313/69‑1 (MQ=255)

GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAC  >  W3110S.gb/4563523‑4563650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`