| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,085 | T→C | 37.5% | S233S (AGT→AGC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,085 | 0 | T | C | 37.5% | 36.7 / 51.9 | 48 | S233S (AGT→AGC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (13/17); new base C (6/12); total (19/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAC > W3110S.gb/71016‑71154 | tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc < 1:1292103/71‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGATGACCAACGTATCAGCCAGGCGa < 1:1567134/71‑1 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg < 1:1702549/69‑1 (MQ=255) tCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt < 1:1583579/61‑1 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:193084/70‑1 (MQ=255) tCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgct < 1:1088847/70‑1 (MQ=255) tCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGAATTAGTCAGGCGAAgctgct < 1:1325499/70‑1 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:1220072/71‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg > 1:936518/1‑71 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGAGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:1198936/71‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGaa > 1:1153614/1‑57 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCa < 1:190994/47‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg < 1:362460/51‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt < 1:1553282/45‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGa < 1:1106285/52‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg < 1:1772567/63‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:1587896/65‑1 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCttt > 1:378156/1‑59 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc > 1:723223/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAcc > 1:1811410/1‑70 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1586818/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:504736/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:480224/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:1212758/71‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg < 1:2183866/47‑1 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg < 1:916111/43‑1 (MQ=37) tAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg > 1:1595009/1‑57 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:2195743/64‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:588106/61‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGa > 1:1373399/1‑61 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGa > 1:1844775/1‑49 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa > 1:752692/1‑55 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:536925/59‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:195739/1‑59 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGc < 1:1084591/69‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa > 1:504204/1‑71 (MQ=255) gcgcGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtc < 1:1362532/70‑1 (MQ=255) gcgcGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgt < 1:1075670/69‑1 (MQ=255) cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg < 1:119649/71‑1 (MQ=255) cgAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtc > 1:934848/1‑71 (MQ=255) gAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtc > 1:1689097/1‑70 (MQ=255) ggcccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGAACTACCCGGATGCCTATCGCCACCGTCGGTCGc > 1:2128127/4‑70 (MQ=255) gACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCaa > 1:926012/1‑71 (MQ=255) ccAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTa < 1:1206202/50‑1 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:1860587/1‑48 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:706743/1‑71 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:1104435/1‑71 (MQ=255) gCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGt > 1:1249649/1‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:462567/62‑1 (MQ=255) gTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc < 1:1719171/71‑1 (MQ=255) tCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:1622363/1‑62 (MQ=255) | TCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAC > W3110S.gb/71016‑71154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |