| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 324,001 | C→T | 12.3% | T138I (ACC→ATC) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 324,001 | 0 | C | T | 12.3% | 231.9 / 20.1 | 81 | T138I (ACC→ATC) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base C (25/46); new base T (7/3); total (32/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
AAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAA > minE/323932‑324071 | aaGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGAcc < 1:1083663/71‑1 (MQ=255)aaGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGAc > 1:2471794/1‑70 (MQ=255)aaGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGAc > 1:1454971/1‑70 (MQ=255) gACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGt < 1:3724489/71‑1 (MQ=255) gACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGt < 1:717206/71‑1 (MQ=255) gACAGCCAGCTGATCGGTCAGTTTGGAGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGt < 1:2689722/71‑1 (MQ=255) aCAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTg > 1:321531/1‑71 (MQ=255) tagccagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGATCGTGc > 1:879725/2‑71 (MQ=255) tagccagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGATCGTGc > 1:874651/2‑71 (MQ=255) ccagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTa > 1:895070/1‑71 (MQ=255) cagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGAc > 1:746616/1‑62 (MQ=255) cagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTAc > 1:1392314/1‑71 (MQ=255) cTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcg > 1:1755949/1‑71 (MQ=255) tGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcgc > 1:1013247/1‑71 (MQ=255) tGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcgc > 1:180776/1‑71 (MQ=255) tGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcgc > 1:3545207/1‑71 (MQ=255) gATCGGGCAGTTTGGTGTTGGGTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGAcc < 1:1943708/58‑1 (MQ=255) ggTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCgg > 1:3618582/1‑70 (MQ=255) ggTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCgg > 1:3663737/1‑70 (MQ=255) gTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCa < 1:1396134/71‑1 (MQ=255) gTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCa < 1:1206334/71‑1 (MQ=255) gTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGGACTCGCGCGGCa < 1:1488274/71‑1 (MQ=255) gTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCCTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCa < 1:1944171/71‑1 (MQ=255) tCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcgcg < 1:1934966/65‑1 (MQ=255) tCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcgcg < 1:3680030/65‑1 (MQ=255) cAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTcgcg < 1:3684728/64‑1 (MQ=255) gTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGc > 1:2431946/1‑66 (MQ=255) tttGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGa < 1:2835781/71‑1 (MQ=255) tttGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGa < 1:797425/71‑1 (MQ=255) tGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGaa < 1:3188702/70‑1 (MQ=255) ggTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGaaa < 1:1994368/70‑1 (MQ=255) ggTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGaaa < 1:3163670/70‑1 (MQ=255) gtgtTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCGGCAGGCGaaaaa < 1:755788/71‑1 (MQ=255) gtgtTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGaaaaa < 1:3740626/71‑1 (MQ=255) tgtTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGaaa < 1:1984206/68‑1 (MQ=255) ttGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAAc > 1:798107/1‑69 (MQ=255) ggTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCGGCAGGCGAAAAACAAg > 1:3321398/1‑70 (MQ=255) ggTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAg > 1:2033130/1‑70 (MQ=255) gTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTa > 1:1045021/1‑45 (MQ=255) gTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTa > 1:2392519/1‑45 (MQ=255) gTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaa < 1:2786219/71‑1 (MQ=255) tCTACTCTGCGTTTATCGTGGCCGAGAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGa < 1:1812649/67‑1 (MQ=255) tCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaaaa < 1:2126242/70‑1 (MQ=255) tCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAt > 1:3305377/1‑71 (MQ=255) tACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACt > 1:2243924/1‑42 (MQ=255) ctctGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATg < 1:3061758/68‑1 (MQ=255) ctctGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATg < 1:1022999/68‑1 (MQ=255) ctctGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGc < 1:3390496/70‑1 (MQ=255) ttcGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtct > 1:2248887/3‑71 (MQ=255) cGTTTATCGTGTCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttc < 1:2178232/71‑1 (MQ=255) cGTTTATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttc < 1:2868356/71‑1 (MQ=255) cGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATg < 1:575984/63‑1 (MQ=255) cGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttc < 1:1105125/71‑1 (MQ=255) cGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttc > 1:3121109/1‑71 (MQ=255) tttATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttct < 1:2347428/70‑1 (MQ=255) tttATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaaaa < 1:1656973/59‑1 (MQ=255) tATCGTGGCCGACAAAGTGATCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTgg > 1:2016130/1‑70 (MQ=255) tATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttct < 1:2360979/68‑1 (MQ=255) tCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttct > 1:1105128/1‑66 (MQ=255) tCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtcttc > 1:1961253/1‑65 (MQ=255) tCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGa < 1:1135463/70‑1 (MQ=255) gTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaa > 1:2741785/1‑51 (MQ=255) gTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaa > 1:3115552/1‑51 (MQ=255) tGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCg > 1:1035354/1‑71 (MQ=255) aggCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtct < 1:422659/59‑1 (MQ=255) gCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGc < 1:3149431/71‑1 (MQ=255) gCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGc < 1:3144713/71‑1 (MQ=255) ccGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCt < 1:370310/71‑1 (MQ=255) ccGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCt < 1:1050401/71‑1 (MQ=255) cGACAAAGTGACCGTGCGTGCTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTg > 1:3235992/1‑71 (MQ=255) gACAAAGTGATCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAAt > 1:1156613/1‑64 (MQ=255) gACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCg < 1:35796/52‑1 (MQ=255) gACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCg < 1:3262282/52‑1 (MQ=255) aCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaaaa < 1:3573857/46‑1 (MQ=255) aCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaa < 1:1682463/44‑1 (MQ=255) aCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaa < 1:3464251/44‑1 (MQ=255) aCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGaa < 1:1623541/44‑1 (MQ=255) aCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCg < 1:2969452/51‑1 (MQ=255) aCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGtc < 1:2753042/53‑1 (MQ=255) aaaGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATg > 1:3821342/1‑46 (MQ=255) aaaGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATg > 1:3116006/1‑46 (MQ=255) aaaGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGa < 1:2072524/71‑1 (MQ=255) aaaGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGa < 1:3178305/71‑1 (MQ=255) aGTGACCGTGAGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAg > 1:1242697/1‑71 (MQ=255) tGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGt < 1:1730150/71‑1 (MQ=255) tGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGt < 1:2197916/71‑1 (MQ=255) ccGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGaa < 1:765725/71‑1 (MQ=255) | AAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAA > minE/323932‑324071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |