| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 483,085 | G→T | 11.5% | A97S (GCG→TCG) | tolA → | membrane anchored protein in TolA‑TolQ‑TolR complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 483,085 | 0 | G | T | 11.5% | 297.7 / 12.0 | 93 | A97S (GCG→TCG) | tolA | membrane anchored protein in TolA‑TolQ‑TolR complex |
| Reads supporting (aligned to +/- strand): ref base G (39/43); new base T (4/7); total (43/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAG > minE/483015‑483153 | cgcgAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAg > 1:1497983/1‑71 (MQ=255) aaGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGc > 1:32015/1‑71 (MQ=255) aaGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGc > 1:2431597/1‑71 (MQ=255) gCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTg < 1:1008541/71‑1 (MQ=255) gCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTg < 1:2507268/71‑1 (MQ=255) gCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTg < 1:2217870/71‑1 (MQ=255) gTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGaa < 1:4046058/71‑1 (MQ=255) ttCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGc < 1:2799106/63‑1 (MQ=255) ttCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGaa > 1:1867434/1‑70 (MQ=255) cTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGaa < 1:1066599/68‑1 (MQ=255) cTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGaa < 1:332910/68‑1 (MQ=255) cTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACa < 1:406893/70‑1 (MQ=255) cTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAg > 1:2276550/1‑71 (MQ=255) cTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAg > 1:2473065/1‑71 (MQ=255) cTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAG‑AACAAGCGGCTGAACAg > 1:120830/1‑70 (MQ=255) gatgaACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAg < 1:792395/69‑1 (MQ=255) atgaACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAgg < 1:3782009/69‑1 (MQ=255) tgaACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAAc > 1:292578/1‑71 (MQ=255) aCAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGcc < 1:769777/71‑1 (MQ=255) cAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCt > 1:1907347/1‑71 (MQ=255) gcgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAAc < 1:2261317/59‑1 (MQ=255) gcgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAAc < 1:2739697/59‑1 (MQ=255) cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTg < 1:3086972/69‑1 (MQ=255) cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTg > 1:3390353/1‑69 (MQ=255) cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTg > 1:3801888/1‑69 (MQ=255) cgcAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGaa < 1:576094/71‑1 (MQ=255) aaGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCa < 1:1322597/71‑1 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAAc < 1:3884079/53‑1 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGaa > 1:2529662/1‑58 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGaa > 1:3632287/1‑58 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGc > 1:968882/1‑61 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCa < 1:2305067/69‑1 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCa < 1:1772861/69‑1 (MQ=255) gATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAAc < 1:3517575/71‑1 (MQ=255) gATGAAGGAACAGCAGGCTGCTCAAGAACTGAGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCaa > 1:1341677/1‑70 (MQ=255) aTGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGaa > 1:2096458/1‑51 (MQ=255) tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACtt < 1:4058125/71‑1 (MQ=255) tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACtt < 1:3797890/71‑1 (MQ=255) tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACtt > 1:2323840/1‑71 (MQ=255) tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACtt > 1:756138/1‑71 (MQ=255) tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACtt < 1:3205433/71‑1 (MQ=255) tGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACt > 1:3644426/1‑70 (MQ=255) gAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACtt > 1:468046/1‑70 (MQ=255) ggAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTgaga < 1:2115760/71‑1 (MQ=255) ggAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTgaga < 1:3401906/71‑1 (MQ=255) aaCAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAAct > 1:1855739/1‑51 (MQ=255) aaCAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTg > 1:1850953/1‑66 (MQ=255) aaCAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTg > 1:3186739/1‑66 (MQ=255) aaCAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGaaa < 1:2600902/71‑1 (MQ=255) gcagGCTGCTGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGc < 1:2560340/71‑1 (MQ=255) cagGCTGCTGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCg < 1:422553/71‑1 (MQ=255) cagGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCg < 1:1728808/71‑1 (MQ=255) cagGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCg < 1:2785604/71‑1 (MQ=255) gctgctGAAGAACTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTgaga < 1:294834/61‑1 (MQ=255) gctgctGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGGAAGAGCGGtt < 1:1070019/71‑1 (MQ=255) gctgctGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGc < 1:416466/67‑1 (MQ=255) gctgctGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGtt < 1:1642867/71‑1 (MQ=255) gctgctGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGtt < 1:3158267/71‑1 (MQ=255) tgctgAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCgg > 1:3242661/1‑67 (MQ=255) tgAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAag < 1:3409987/59‑1 (MQ=255) tgAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCgg > 1:1940276/1‑71 (MQ=255) gaagaaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAAc > 1:2422596/1‑49 (MQ=255) aagaaCTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCt > 1:2325543/1‑71 (MQ=255) aagaaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGc > 1:3423635/1‑38 (MQ=255) aagaaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTga < 1:2805216/52‑1 (MQ=255) aagaaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCt > 1:2080774/1‑71 (MQ=255) agaaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTgaga < 1:3569826/53‑1 (MQ=255) gaaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCt > 1:1405850/1‑69 (MQ=255) aaCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAg < 1:353953/71‑1 (MQ=255) aaCTGCGTGAGAAACAAGAGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAg < 1:881017/71‑1 (MQ=255) aCTGCGTGAGAAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAg > 1:957627/1‑70 (MQ=255) aCTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAg > 1:204216/1‑70 (MQ=255) cTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCaa < 1:49310/42‑1 (MQ=255) cTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCaa < 1:1012336/42‑1 (MQ=255) cTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCaa < 1:2822444/42‑1 (MQ=255) cTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCgg < 1:3821479/64‑1 (MQ=255) cTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCgg < 1:582112/64‑1 (MQ=255) gCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACt > 1:3050519/1‑42 (MQ=255) gCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACt > 1:2200892/1‑42 (MQ=255) gCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAg > 1:749969/1‑59 (MQ=255) gCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAg > 1:2200819/1‑70 (MQ=255) cGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAg > 1:1447791/1‑69 (MQ=255) agaAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGa > 1:4097648/1‑64 (MQ=255) agaAACAAGCGGCTGAACAGGAACGCCTGAAGCaa > 1:2441089/1‑35 (MQ=255) agaAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTga > 1:1848999/1‑40 (MQ=255) gaAACAATCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTc < 1:2666777/59‑1 (MQ=255) gaAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGaaa > 1:1997579/1‑70 (MQ=255) aaaCAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCg > 1:2879155/1‑54 (MQ=255) aaaCAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCg > 1:2849317/1‑54 (MQ=255) aaaCAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGaaaaa < 1:3132528/71‑1 (MQ=255) aaaCAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGaaaaa < 1:3127725/71‑1 (MQ=255) aaaCAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGaaaaa < 1:1339277/71‑1 (MQ=255) aaCAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGaaaaa < 1:1876215/70‑1 (MQ=255) aaGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAg > 1:173886/1‑71 (MQ=255) gCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAag > 1:2462753/1‑37 (MQ=255) | CGCGAAGCGTTCTGATGAACAGCGCAAGATGAAGGAACAGCAGGCTGCTGAAGAACTGCGTGAGAAACAAGCGGCTGAACAGGAACGCCTGAAGCAACTTGAGAAAGAGCGGTTAGCGGCTCAGGAGCAGAAAAAGCAG > minE/483015‑483153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |