| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 977,473 | C→T | 14.5% | D558N (GAT→AAT) | dcp ← | dipeptidyl carboxypeptidase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 977,473 | 0 | C | T | 14.5% | 186.4 / 21.7 | 69 | D558N (GAT→AAT) | dcp | dipeptidyl carboxypeptidase II |
| Reads supporting (aligned to +/- strand): ref base C (23/36); new base T (6/4); total (29/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
AGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTT > minE/977403‑977541 | aGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATc > 1:371887/1‑71 (MQ=255)aGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATc > 1:3263749/1‑71 (MQ=255) gcCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATc > 1:1441273/1‑68 (MQ=255) gcCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCgag < 1:2825844/71‑1 (MQ=255) gcCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCgag < 1:2726547/71‑1 (MQ=255) gcCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCgag < 1:1841752/71‑1 (MQ=255) cGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCgag < 1:1047425/68‑1 (MQ=255) cGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCgag < 1:2777390/68‑1 (MQ=255) cGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCgag < 1:2745954/68‑1 (MQ=255) cGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAg < 1:762404/71‑1 (MQ=255) gCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGt > 1:993939/1‑71 (MQ=255) ttCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTgcgg > 1:2384066/1‑71 (MQ=255) aaaaTCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTgcggcg < 1:874905/70‑1 (MQ=255) aaaTCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTGCGGCGCt < 1:1479959/71‑1 (MQ=255) aaaTCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCt < 1:688794/71‑1 (MQ=255) aaTCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCt > 1:986605/1‑70 (MQ=255) aaTCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCt > 1:1012080/1‑70 (MQ=255) atcGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAg > 1:3484672/1‑69 (MQ=255) atcGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGc < 1:1864764/70‑1 (MQ=255) atcGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGc < 1:2772507/70‑1 (MQ=255) tcGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTgc < 1:1740731/59‑1 (MQ=255) aTCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGc > 1:3875417/1‑71 (MQ=255) aTCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGc < 1:3268568/71‑1 (MQ=255) aTCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGc < 1:980303/71‑1 (MQ=255) aTTGCTTCGTTTTCTTCCAGGCAATGCCAGCTCATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatctc < 1:912091/71‑1 (MQ=255) aTTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTGCGGCGCTAAGCAGTTCGct < 1:437310/65‑1 (MQ=255) aTTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatctc < 1:35668/71‑1 (MQ=255) ttGCTTCGTTTTCTTCCAGGCAATGCCAGCGCGTATTGAGAAGTGCGGCGCt < 1:1908428/52‑1 (MQ=255) ttGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTaa < 1:2145324/54‑1 (MQ=255) ttGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGct < 1:770627/64‑1 (MQ=255) ttGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGAGCTaa < 1:243895/54‑1 (MQ=255) tGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatctca > 1:1898933/1‑70 (MQ=255) gCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATc > 1:2863402/1‑35 (MQ=255) gCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATc > 1:1884854/1‑35 (MQ=255) gCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTgcgg > 1:1442985/1‑46 (MQ=255) gCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTgcgg > 1:440493/1‑46 (MQ=255) gCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTgcgg > 1:701760/1‑46 (MQ=255) gCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATa > 1:150078/1‑71 (MQ=255) cTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATa < 1:2479146/70‑1 (MQ=255) ttCGTTTTCTTCCAGGCAATGCCAGCGCATATTGAGAAGTgccg < 1:2376546/44‑3 (MQ=39) cGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCa > 1:964789/1‑51 (MQ=255) cGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCa > 1:211271/1‑51 (MQ=255) cGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCa > 1:218216/1‑51 (MQ=255) gTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCt < 1:538068/70‑1 (MQ=255) ttttCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCtt < 1:2131178/70‑1 (MQ=255) ttcttcCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCa > 1:1334202/1‑47 (MQ=255) ttcttcCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATa > 1:1673144/1‑63 (MQ=255) ttcttcCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgt > 1:1500598/1‑71 (MQ=255) ttcttcCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgt > 1:1750486/1‑71 (MQ=255) ccAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgttg < 1:1413324/68‑1 (MQ=255) ccAGGCAATGCCACCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgttg < 1:831529/68‑1 (MQ=255) cAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGaa > 1:3939846/1‑69 (MQ=255) cAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGaa > 1:1547304/1‑69 (MQ=255) aGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatct < 1:372635/52‑1 (MQ=255) aGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGctcatct < 1:2901565/52‑1 (MQ=255) aGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAg < 1:2242138/71‑1 (MQ=255) gCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGc < 1:3706760/71‑1 (MQ=255) aaTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgt > 1:301512/1‑60 (MQ=255) aTGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTgg < 1:3412419/71‑1 (MQ=255) ccAGCGCATATCGTGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt < 1:936037/71‑1 (MQ=255) ccAGCGCATATCGTGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt < 1:3515422/71‑1 (MQ=255) cAGCGCATATTGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCAt > 1:3427294/1‑70 (MQ=255) gcgcATATTGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTAc > 1:3051178/1‑71 (MQ=255) gcATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgtt > 1:2754856/1‑52 (MQ=255) gcATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTttgtt > 1:1689727/1‑52 (MQ=255) gcATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGc < 1:1335307/71‑1 (MQ=255) atatCGAGAAGTGCGGCGCTAAGCAGTTCGctcatc > 1:3759039/1‑36 (MQ=255) atatCGAGAAGTGCGGCGCTAAGCAGTTCGctcat > 1:3142823/1‑35 (MQ=255) atatCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAt < 1:2609445/71‑1 (MQ=255) tatCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGc < 1:1421104/57‑1 (MQ=255) atCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGc < 1:1923113/56‑1 (MQ=255) atCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGc < 1:3088053/56‑1 (MQ=255) tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAttt > 1:1805046/1‑70 (MQ=255) tCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCAttt > 1:886995/1‑70 (MQ=255) | AGCGCCCGCAATTCAAAATCATCGACATCCTGCATTGCTTCGTTTTCTTCCAGGCAATGCCAGCGCATATCGAGAAGTGCGGCGCTAAGCAGTTCGCTCATCTCATACCCTTTGTTGAACAGGCTGGCATTACGCATTT > minE/977403‑977541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |