| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 992,906 | G→T | 9.5% | intergenic (+95/‑100) | dmsD → / → clcB | twin‑argninine leader‑binding protein for DmsA and TorA/predicted voltage‑gated chloride channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 992,906 | 0 | G | T | 9.5% | 267.9 / 7.2 | 82 | intergenic (+95/‑100) | dmsD/clcB | twin‑argninine leader‑binding protein for DmsA and TorA/predicted voltage‑gated chloride channel |
| Reads supporting (aligned to +/- strand): ref base G (37/37); new base T (4/4); total (41/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCAT > minE/992838‑992975 | aCGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCt > 1:2262661/1‑71 (MQ=255)aCGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCt > 1:3725771/1‑71 (MQ=255) gATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCttt < 1:2877853/71‑1 (MQ=255) ttACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTg > 1:596521/1‑71 (MQ=255) tACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGc > 1:361205/1‑71 (MQ=255) tACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGc > 1:1339230/1‑71 (MQ=255) aCGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCg > 1:2372550/1‑71 (MQ=255) cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg < 1:6547/71‑1 (MQ=255) cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg < 1:1602457/71‑1 (MQ=255) cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg < 1:2693102/71‑1 (MQ=255) cgcgCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCgg > 1:3707591/1‑71 (MQ=255) cgcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTTCTTTTGCGGCt < 1:997050/71‑1 (MQ=255) gcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg < 1:2237561/71‑1 (MQ=255) gcAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg > 1:4004467/1‑71 (MQ=255) aaTCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTgg > 1:2501455/1‑70 (MQ=255) cGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCctct > 1:3417990/1‑71 (MQ=255) cGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCctct > 1:3520534/1‑71 (MQ=255) cGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCctc < 1:1001686/70‑1 (MQ=255) ggCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtt < 1:756727/71‑1 (MQ=255) ggCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtt < 1:428863/71‑1 (MQ=255) ataGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGGACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCg > 1:516637/1‑70 (MQ=255) ataGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGcg > 1:3627714/1‑59 (MQ=255) ataGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtttt > 1:1290407/1‑66 (MQ=255) ataGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCtttt > 1:3246294/1‑66 (MQ=255) ataGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGcc < 1:479638/69‑1 (MQ=255) taGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGc < 1:1378101/54‑1 (MQ=255) taGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGc > 1:2149593/1‑70 (MQ=255) taGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCa < 1:1842728/71‑1 (MQ=255) taGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCa < 1:3308820/71‑1 (MQ=255) gTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaaa < 1:3818679/71‑1 (MQ=255) gTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaaa < 1:3485326/71‑1 (MQ=255) gTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaaaa < 1:4085939/71‑1 (MQ=255) gTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaa < 1:3909488/69‑1 (MQ=255) aGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTc < 1:162553/71‑1 (MQ=255) tgtCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGc < 1:3873481/71‑1 (MQ=255) tCACGTTTTTTTATCTCTTAAAGCACGCACTGCtt < 1:1600323/35‑1 (MQ=255) tCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGccc > 1:705852/1‑71 (MQ=255) cACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaa < 1:2224081/59‑1 (MQ=255) aCGTTTTTTTATCTCTTAAAGCACGCACTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCgt > 1:3967814/1‑71 (MQ=255) aCGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCttt > 1:1385088/1‑50 (MQ=255) aCGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCgt < 1:2773871/71‑1 (MQ=255) aCGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCgt < 1:1448535/71‑1 (MQ=255) aCGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCgt > 1:2155932/1‑71 (MQ=255) gTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaa < 1:2014722/56‑1 (MQ=255) gTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCaa < 1:1685812/56‑1 (MQ=255) gTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCACgtgt < 1:1184661/71‑1 (MQ=255) tttttttATCTCTTAAAGCACGCACTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGtt < 1:3407745/71‑1 (MQ=255) ttttttATCTCTTAAAGCACGCACTGCTTTTGCGGCt < 1:1592278/37‑1 (MQ=255) ttttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGc > 1:365523/1‑52 (MQ=255) ttttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt > 1:943058/1‑71 (MQ=255) ttttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGtt > 1:946219/1‑70 (MQ=255) tttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt > 1:3743813/1‑70 (MQ=255) tttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt < 1:3682801/70‑1 (MQ=255) tttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGttt > 1:181123/1‑70 (MQ=255) ttttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa > 1:3574040/1‑71 (MQ=255) tttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCa < 1:533264/70‑1 (MQ=255) ttATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAtt > 1:1279997/1‑71 (MQ=255) tctcTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc > 1:3756978/1‑71 (MQ=255) ctTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCa > 1:284082/1‑70 (MQ=255) aaaGCACGCACTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc > 1:3407591/1‑71 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGt > 1:1716354/1‑47 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGccc > 1:295905/1‑66 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAtt < 1:1129963/69‑1 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAt < 1:3008141/68‑1 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc > 1:4010896/1‑71 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc > 1:1005770/1‑71 (MQ=255) aaaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc > 1:3513194/1‑71 (MQ=255) aaGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCt < 1:519785/71‑1 (MQ=255) aGCACGCACTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg < 1:1464475/71‑1 (MQ=255) cacgcacTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGc > 1:1322933/1‑70 (MQ=255) cacgcacTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCt < 1:2804440/71‑1 (MQ=255) cacgcacTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCt < 1:2484936/71‑1 (MQ=255) acgcacTTCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCt > 1:2006597/1‑70 (MQ=255) acgcacTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCt > 1:3008367/1‑70 (MQ=255) gcacTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCAt < 1:1774923/71‑1 (MQ=255) cacTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg < 1:1485289/71‑1 (MQ=255) cacTGCTTTTGCGGCTGGCCTCTTTCGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATg < 1:1735947/71‑1 (MQ=255) acTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGccc < 1:1936456/57‑1 (MQ=255) acTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCAt > 1:500110/1‑69 (MQ=255) acTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCAt > 1:1327819/1‑69 (MQ=255) cTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGca < 1:3315272/71‑1 (MQ=255) tGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGcat > 1:3558542/1‑71 (MQ=255) gCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc < 1:3891218/53‑1 (MQ=255) gCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGcat > 1:640556/1‑70 (MQ=255) | ACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCAT > minE/992838‑992975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |