| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,742 | G→A | 12.1% | G109G (GGC→GGT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,742 | 0 | G | A | 12.1% | 193.3 / 13.9 | 66 | G109G (GGC→GGT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (31/27); new base A (4/4); total (35/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGC > minE/1015672‑1015805 | gcgcCAGCGCCGCTTCATAACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTg < 1:3858535/71‑1 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc < 1:1555891/71‑1 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc < 1:1550816/71‑1 (MQ=255) cAGCGCCGCTTCATCTCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCg < 1:4120867/70‑1 (MQ=255) cAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGTGTTACCgg > 1:3997553/1‑71 (MQ=255) cAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg > 1:3433433/1‑71 (MQ=255) gcgcCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa > 1:440759/1‑71 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc > 1:1570852/1‑71 (MQ=255) ccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATcc < 1:1267889/71‑1 (MQ=255) gcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt > 1:1993825/1‑70 (MQ=255) ttCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:1292530/1‑70 (MQ=255) tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:1416971/1‑69 (MQ=255) tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:478473/1‑69 (MQ=255) catcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGAc < 1:3461133/69‑1 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc > 1:904290/1‑57 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAg < 1:453733/70‑1 (MQ=255) tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGt < 1:2080477/71‑1 (MQ=255) ccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTgg > 1:2748941/1‑68 (MQ=255) ccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:187228/71‑1 (MQ=255) ccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCCGACAGGTTGGCAg < 1:916815/71‑1 (MQ=255) caccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTgg < 1:2739178/67‑1 (MQ=255) accGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAg < 1:288501/61‑1 (MQ=255) accGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:3190041/1‑71 (MQ=255) ccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:883492/1‑70 (MQ=255) ccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:3891143/1‑70 (MQ=255) cGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAAc < 1:3544685/70‑1 (MQ=255) ggTCCATACACGCTGCCCTTTTTTACTAACAATAATCGCGGTGCCTGTATCCTGACAGGTTGGCAGAACGc < 1:888784/71‑1 (MQ=255) ggTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc > 1:3795709/1‑71 (MQ=255) ggTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc > 1:1838300/1‑71 (MQ=255) cATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCtt < 1:434743/70‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGAc > 1:1635468/1‑50 (MQ=255) cacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgcc > 1:1674049/1‑71 (MQ=255) cacGCTGCCCTTTTTTACCAACAATAATCGCGGTACCGGTa > 1:938722/1‑41 (MQ=255) acGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCg > 1:736277/1‑37 (MQ=255) acGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCg > 1:2487963/1‑37 (MQ=255) acGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCg > 1:852895/1‑37 (MQ=255) cGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTCGCAGAACGCCTTTcgccgc < 1:314583/71‑1 (MQ=255) gCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACg > 1:2120855/1‑59 (MQ=255) tGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:2795689/1‑42 (MQ=255) cccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGAt < 1:4119048/69‑1 (MQ=255) ccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:623639/50‑1 (MQ=255) ccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTc < 1:302048/71‑1 (MQ=255) cTTTTTTACCAACAATAATCGCGGTACCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg < 1:1655130/71‑1 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:1171732/1‑69 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:2380246/1‑69 (MQ=255) ttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCgg < 1:1737907/69‑1 (MQ=255) ttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAg > 1:1585987/1‑71 (MQ=255) tttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCgg < 1:3447688/68‑1 (MQ=255) tttACCAACAATAATCGCGGTACCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:3131853/1‑62 (MQ=255) tttACCAACAATAATCGCGGTACCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:1581633/1‑62 (MQ=255) aCCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTAc > 1:916538/1‑71 (MQ=255) ccAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:595295/41‑1 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAg > 1:2982685/1‑71 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAg > 1:3062939/1‑71 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAg > 1:1587391/1‑71 (MQ=255) caaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg < 1:500676/71‑1 (MQ=255) caaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAg > 1:3006653/1‑70 (MQ=255) aataatCGCTGTGCCGGTATCCTGACAGGTCGGCAGAACGCCTTTcgccgc < 1:3438468/51‑1 (MQ=39) aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg > 1:2158346/1‑70 (MQ=255) taatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt < 1:3171930/71‑1 (MQ=255) taatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAt < 1:1609567/71‑1 (MQ=255) atCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:1192979/47‑1 (MQ=255) atCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg > 1:263159/1‑71 (MQ=255) atCGCGGTACCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAAtt < 1:208523/70‑1 (MQ=255) atCGCGGTACCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg < 1:2743737/71‑1 (MQ=255) tCGCGGTACCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGc < 1:1907472/71‑1 (MQ=255) tGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGa < 1:142306/49‑1 (MQ=255) | GCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGC > minE/1015672‑1015805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |