| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,194,880 | C→T | 14.6% | A102T (GCG→ACG) | yeaZ ← | predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,194,880 | 0 | C | T | 14.6% | 129.0 / 13.3 | 48 | A102T (GCG→ACG) | yeaZ | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base T (5/2); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTT > minE/1194813‑1194948 | cAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGcccc < 1:4005155/71‑1 (MQ=255) aGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCt > 1:303020/1‑71 (MQ=255) aaaCTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGTCCCTTgc > 1:1278235/1‑71 (MQ=255) cTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGcc < 1:2092190/71‑1 (MQ=255) cGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCa < 1:478016/69‑1 (MQ=255) cccATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGt < 1:395702/71‑1 (MQ=255) cccATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGt < 1:1680599/71‑1 (MQ=255) ccATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGt < 1:2684760/70‑1 (MQ=255) cATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCAt > 1:3676150/1‑66 (MQ=255) tCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGTCCCTTGCGCCATCGTCAt > 1:1241144/1‑69 (MQ=255) cgcgTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCg > 1:1223588/1‑71 (MQ=255) cgcgTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCg > 1:3359520/1‑71 (MQ=255) gcgTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGTCCCTTGCGCCATCGTCATTAGCGt < 1:2251377/71‑1 (MQ=255) gcgTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGt > 1:1173109/1‑71 (MQ=255) cgTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTg < 1:3904161/71‑1 (MQ=255) cgTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTg > 1:469159/1‑71 (MQ=255) tCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGga < 1:1985630/71‑1 (MQ=255) tCAATGGCTGCCAGCACGCGGGTTCCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGga < 1:3568465/71‑1 (MQ=255) aTGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGAc > 1:800181/1‑71 (MQ=255) tGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACg > 1:442762/1‑71 (MQ=255) tGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACg > 1:1996267/1‑71 (MQ=255) gCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGTCCCTTgcgc > 1:3609889/1‑47 (MQ=255) gCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGTCCCTTgc > 1:3360053/1‑45 (MQ=255) cTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCa < 1:2601683/71‑1 (MQ=255) tGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCaa > 1:1705402/1‑71 (MQ=255) aGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAatcat < 1:3335520/71‑1 (MQ=255) cACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCAt < 1:1408902/48‑1 (MQ=255) cACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCg > 1:2247775/1‑71 (MQ=255) cgcgGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGt < 1:1298982/71‑1 (MQ=255) ttGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTc < 1:3713716/38‑1 (MQ=255) ttGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTc < 1:3043389/38‑1 (MQ=255) cgcCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAAt < 1:3315980/65‑1 (MQ=255) tccGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGt < 1:3359994/60‑1 (MQ=255) cccGTTTTTGCGCCACGTCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGt < 1:1404750/60‑1 (MQ=255) ccGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGcc < 1:2143103/71‑1 (MQ=255) cGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGgaga > 1:3589201/1‑45 (MQ=255) cGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGgaga > 1:1677981/1‑45 (MQ=255) cGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGgaga > 1:3668976/1‑45 (MQ=255) cGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGgaca > 1:1407346/1‑43 (MQ=255) gTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTg < 1:516537/40‑1 (MQ=255) gTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCaa < 1:112012/71‑1 (MQ=255) tttttGCGCCACGTCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAg > 1:3359334/1‑71 (MQ=255) tttttGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAg > 1:157239/1‑71 (MQ=255) tttttGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATAATCGGTAATTCCGCGCCAAg > 1:2814656/1‑71 (MQ=255) tttGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTg > 1:2901085/1‑37 (MQ=255) tttGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTg > 1:2434413/1‑37 (MQ=255) tttGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTg > 1:112006/1‑37 (MQ=255) tttGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAgcg > 1:2582088/1‑71 (MQ=255) cGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCtt > 1:1287402/1‑71 (MQ=255) | CAGTAAACTTCGCCCATTCGCGCGTCAATGGCTGCCAGCACGCGGGTTGCGCCGTTTTTGCGCCACGCCCCTTGCGCCATCGTCATTAGCGTGGAGACGCCAATCATCGGTAATTCCGCGCCAAGCGCCAGCCCTT > minE/1194813‑1194948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |