| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,224,379 | G→A | 13.7% | E81K (GAA→AAA) | yebU → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,224,379 | 0 | G | A | 13.7% | 203.6 / 20.6 | 73 | E81K (GAA→AAA) | yebU | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (33/30); new base A (4/6); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 | |||||||||||
GCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAGGAAGCCAGTT > minE/1224310‑1224448 | gCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATg > 1:1902130/1‑70 (MQ=255)gCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGa < 1:3851518/71‑1 (MQ=255)gCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGa < 1:2391948/71‑1 (MQ=255) tCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGa < 1:3445765/69‑1 (MQ=255) cTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGAt > 1:3865326/1‑71 (MQ=255) ttATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATg > 1:932772/1‑71 (MQ=255) aTGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATg < 1:3223874/69‑1 (MQ=255) aTGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATGCa > 1:1012465/1‑71 (MQ=255) aTGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATGCa > 1:1482530/1‑71 (MQ=255) tGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCAt > 1:1979609/1‑71 (MQ=255) tGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCAt > 1:806245/1‑71 (MQ=255) tGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATGCAt < 1:1091330/71‑1 (MQ=255) gACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCa < 1:1075616/70‑1 (MQ=255) gACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCAt < 1:2717784/71‑1 (MQ=255) gACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCAt < 1:2026093/71‑1 (MQ=255) cGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCAtt > 1:3116829/1‑70 (MQ=255) cGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTg > 1:566602/1‑71 (MQ=255) gCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTgg > 1:1213104/1‑71 (MQ=255) tACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTggg > 1:1428429/1‑69 (MQ=255) aCGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGgtag > 1:3171790/1‑71 (MQ=255) cGCCAATTCCGTGGTGTGAGGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGgtag < 1:3527718/70‑1 (MQ=255) aaTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGc > 1:3052230/1‑51 (MQ=255) aaTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGc > 1:208253/1‑51 (MQ=255) aaTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGc > 1:2709742/1‑51 (MQ=255) aaTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGc > 1:156468/1‑51 (MQ=255) aaTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATGCATTGCCATTGGGTAGTAccg < 1:26125/71‑1 (MQ=255) aTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGaa < 1:1767240/45‑1 (MQ=255) aTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGaa < 1:1753012/45‑1 (MQ=255) aTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGaa < 1:565314/45‑1 (MQ=255) aTTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTAccgc > 1:3063471/1‑71 (MQ=255) ttCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTAccgcc > 1:2788995/1‑71 (MQ=255) tCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGa > 1:4029756/1‑45 (MQ=255) tCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGa > 1:214965/1‑45 (MQ=255) gtggtgTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGc < 1:3418053/71‑1 (MQ=255) gtggtgTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATGCATTGCCATTGGGTAGTACCGCCGAGc < 1:2161829/71‑1 (MQ=255) gtggtgTGAAGAAGGTTTCTGGATTGAACGCGACAATAAAGATGCATTGCCATTGGGTAGTACCGCCGAGc < 1:1292011/71‑1 (MQ=255) gtgtGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCa > 1:1924687/1‑43 (MQ=255) tgtgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTAcc < 1:983904/60‑1 (MQ=255) gtgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCa > 1:310547/1‑67 (MQ=255) gtgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCa > 1:372187/1‑67 (MQ=255) tgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTg < 1:989500/49‑1 (MQ=255) tgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTg < 1:283490/49‑1 (MQ=255) tgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTg < 1:1945323/49‑1 (MQ=255) tgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGTATTTa < 1:2066205/70‑1 (MQ=255) tgAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCAttt < 1:774051/69‑1 (MQ=255) gaagaaGGTTTCTGGATTGAACGCGACAATGAAGATGCAtt < 1:3393685/41‑1 (MQ=255) aagaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGgtag > 1:1870051/1‑52 (MQ=255) aagaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGgtag > 1:2593084/1‑52 (MQ=255) aagaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGgtag > 1:2510167/1‑52 (MQ=255) aagaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGgtag > 1:420646/1‑52 (MQ=255) aagaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGt > 1:620943/1‑71 (MQ=255) agaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGc > 1:4032159/1‑41 (MQ=255) agaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGc > 1:3164932/1‑41 (MQ=255) agaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGt < 1:3501187/70‑1 (MQ=255) gaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCAttt < 1:343946/65‑1 (MQ=255) gaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCAttt < 1:436669/65‑1 (MQ=255) gaagGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCAttt < 1:3073388/65‑1 (MQ=255) aagGTTTCTGGATTGAACGCGACAATAAAGATGCATTGCCATTGGGTAGTAccgcc < 1:1527379/56‑1 (MQ=255) tttCTGGATTGAACGCGACAATGAAGATGCATTGCCAtt < 1:4103906/39‑1 (MQ=255) tCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGttt > 1:3806406/1‑71 (MQ=255) cTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGttt < 1:1990605/70‑1 (MQ=255) tGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTa < 1:3984056/57‑1 (MQ=255) gATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTta < 1:1188475/69‑1 (MQ=255) gATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTta < 1:2464165/69‑1 (MQ=255) gATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTta < 1:782196/69‑1 (MQ=255) tGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATAtt > 1:2111407/1‑70 (MQ=255) tGAACGCGACAATAAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATAtt > 1:1296230/1‑70 (MQ=255) tGAACGCGACAATAAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATAtt > 1:2752825/1‑70 (MQ=255) gAACGCGACAATGAAGATGCATTGCCATTGGGTAGTAccgccg > 1:2930351/1‑43 (MQ=255) gcgACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAgg > 1:2934827/1‑69 (MQ=255) gcgACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAgg > 1:1033251/1‑69 (MQ=255) cgACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTatat < 1:3372845/63‑1 (MQ=255) cgACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAGGAAg > 1:839940/1‑71 (MQ=255) tGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAGGAAGCCAGtt < 1:2865985/71‑1 (MQ=255) | GCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAATGAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGTGGCCTGTTTTATATTCAGGAAGCCAGTT > minE/1224310‑1224448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |