| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,225,405 | G→A | 21.0% | E423K (GAG→AAG) | yebU → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,225,405 | 0 | G | A | 21.0% | 125.6 / 28.5 | 57 | E423K (GAG→AAG) | yebU | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (25/20); new base A (4/8); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
AAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTC > minE/1225337‑1225473 | aaGCGGTTATTGCTCTTGCCTCACCCTATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGgaggag < 1:730935/71‑1 (MQ=255)aaGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGgaggag < 1:2733733/71‑1 (MQ=255) ggTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGCAGGAGTgg > 1:2589597/1‑70 (MQ=255) tATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATc > 1:2039508/1‑71 (MQ=255) aTTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTg > 1:982388/1‑65 (MQ=255) aTTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATcg > 1:314537/1‑71 (MQ=255) aTTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATcg > 1:1167567/1‑71 (MQ=255) ttGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTg < 1:3255806/64‑1 (MQ=255) tGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCggagga > 1:726360/1‑60 (MQ=255) tGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCggagga > 1:3868287/1‑60 (MQ=255) tGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATcgcg > 1:703842/1‑71 (MQ=255) gCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCgg > 1:1509755/1‑71 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATcg > 1:1970779/1‑62 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATcg > 1:1144685/1‑62 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATcg > 1:2918469/1‑62 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGgcgc < 1:3937363/69‑1 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGgcgc < 1:578203/69‑1 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGcgcg < 1:2051049/70‑1 (MQ=255) gCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGa > 1:3785461/1‑71 (MQ=255) ccTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGAt < 1:950076/71‑1 (MQ=255) cACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGtt > 1:714986/1‑71 (MQ=255) aCCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGTTATCGCGGGCGCGATGtt < 1:3419134/70‑1 (MQ=255) aCCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGtt > 1:3394374/1‑70 (MQ=255) aCCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGtt < 1:3699510/70‑1 (MQ=255) cccGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTa < 1:2469223/71‑1 (MQ=255) ccGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTa < 1:1669722/70‑1 (MQ=255) ccGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGcgcg < 1:333467/63‑1 (MQ=255) gATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGcgcg < 1:2820768/61‑1 (MQ=255) ataataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCg < 1:3653605/71‑1 (MQ=255) taataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCg > 1:400624/1‑70 (MQ=255) taataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGc < 1:654900/71‑1 (MQ=255) taataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGc < 1:3764455/71‑1 (MQ=255) taataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGc < 1:1828200/71‑1 (MQ=255) aataTGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTTGTATCGCGGGCGCGATGTTTACCCGc > 1:332923/1‑70 (MQ=255) atatGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGgc > 1:3810813/1‑54 (MQ=255) atatGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCa < 1:1607565/70‑1 (MQ=255) atatGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCa < 1:2704457/70‑1 (MQ=255) tatGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTa < 1:3250986/63‑1 (MQ=255) tatGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAg < 1:2641074/71‑1 (MQ=255) tatGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGt < 1:866870/41‑1 (MQ=255) atGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGt < 1:1553111/40‑1 (MQ=255) tGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGc > 1:3077547/1‑70 (MQ=255) tGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCgcgc < 1:419998/71‑1 (MQ=255) cATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCaa > 1:3545878/1‑62 (MQ=255) cATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCaa > 1:3125224/1‑62 (MQ=255) cATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCgcgc > 1:3136160/1‑69 (MQ=255) cATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGcc < 1:3221444/70‑1 (MQ=255) tCGAGCTGACACCGCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTg > 1:3726788/1‑71 (MQ=255) cGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGt > 1:919691/1‑48 (MQ=255) cGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGt > 1:105921/1‑48 (MQ=255) cGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTg < 1:4067730/70‑1 (MQ=255) cGAGCAGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCa > 1:2834973/1‑67 (MQ=255) cTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGga > 1:2420626/1‑71 (MQ=255) acacCGCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGgatg < 1:3132206/70‑1 (MQ=255) gCAGGAAGCGGAGAAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTa > 1:2513493/1‑70 (MQ=255) cAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGttt > 1:1557821/1‑37 (MQ=255) aGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTg > 1:3253658/1‑71 (MQ=255) gAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGatgatg < 1:165314/64‑1 (MQ=255) gAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGt < 1:3136986/71‑1 (MQ=255) gAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGt < 1:2977688/71‑1 (MQ=255) cGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACt > 1:3145327/1‑71 (MQ=255) aggagTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTc > 1:1016731/1‑71 (MQ=255) | AAGCGGTTATTGCTCTTGCCTCACCCGATAATATGAATGCATTCGAGCTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGGGCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGATGTATTGGTTACTTTC > minE/1225337‑1225473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |