| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,326,669 | C→T | 17.1% | L114L (CTG→CTA) | wcaK ← | predicted pyruvyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,326,669 | 0 | C | T | 17.1% | 209.9 / 31.9 | 82 | L114L (CTG→CTA) | wcaK | predicted pyruvyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (28/40); new base T (3/11); total (31/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTG > minE/1326603‑1326739 | cGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGAcagcag < 1:3466805/69‑1 (MQ=255) gCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGTAGgcg > 1:3809160/1‑70 (MQ=255) ccATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACg > 1:1976393/1‑69 (MQ=255) ccATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGaa < 1:2293826/71‑1 (MQ=255) cATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAg < 1:1246386/71‑1 (MQ=255) aTAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGt < 1:773520/71‑1 (MQ=255) aTAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGt < 1:3606789/71‑1 (MQ=255) tAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACg < 1:2286460/66‑1 (MQ=255) agagATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCg > 1:1842776/1‑71 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCgg > 1:80350/1‑71 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:3655468/70‑1 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:2319582/70‑1 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:2764978/70‑1 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:1910264/70‑1 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:1932450/70‑1 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:2672872/70‑1 (MQ=255) gagaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg < 1:2710778/70‑1 (MQ=255) agaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGt < 1:2544471/71‑1 (MQ=255) gaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGt < 1:3399297/70‑1 (MQ=255) gaTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTg < 1:2394554/71‑1 (MQ=255) caTCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGt < 1:1405090/69‑1 (MQ=255) tCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGaa > 1:647687/1‑71 (MQ=255) cGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCg > 1:2744268/1‑65 (MQ=255) cGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGa > 1:202334/1‑69 (MQ=255) cGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAAt > 1:3875931/1‑71 (MQ=255) aCAAAAAACTATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATcc > 1:3811973/1‑71 (MQ=255) aCAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTg < 1:239775/66‑1 (MQ=255) aCAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATcc > 1:3622577/1‑71 (MQ=255) aCAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATcc > 1:2763073/1‑71 (MQ=255) aaaaaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCt < 1:2026565/71‑1 (MQ=255) aaaaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCa > 1:3070489/1‑52 (MQ=255) aaaaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGa < 1:976734/55‑1 (MQ=255) aaaaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCt < 1:1038519/70‑1 (MQ=255) aaaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATcc > 1:2709847/1‑67 (MQ=255) aaaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTgg > 1:2120599/1‑71 (MQ=255) aaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGaa > 1:1297307/1‑63 (MQ=255) aaaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGaa > 1:2257518/1‑63 (MQ=255) aaCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTg > 1:1682417/1‑60 (MQ=255) aCGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggc > 1:3687420/1‑70 (MQ=255) cGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcga < 1:1779545/71‑1 (MQ=255) cGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcga < 1:1426051/71‑1 (MQ=255) cgccgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggc > 1:1431615/1‑70 (MQ=255) cgccgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggc > 1:519124/1‑70 (MQ=255) gccgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggcga < 1:3133473/71‑1 (MQ=255) ccgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggcga > 1:3750079/1‑70 (MQ=255) ccgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggcga > 1:1236325/1‑70 (MQ=255) ccgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggcga < 1:763128/70‑1 (MQ=255) cgACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGata < 1:205675/71‑1 (MQ=255) gACCTGGATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGatat < 1:115765/71‑1 (MQ=255) gACCTGGATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGatat < 1:2122260/71‑1 (MQ=255) gACCTGGATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGatat < 1:3256011/71‑1 (MQ=255) gACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACTAAGTCGGTGAATCCCTGGGCGATGGCGatat > 1:3804770/1‑71 (MQ=255) gACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGatat > 1:442933/1‑71 (MQ=255) gACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGatat > 1:3781369/1‑71 (MQ=255) aCCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTg < 1:1797269/48‑1 (MQ=255) aCCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATAtt < 1:449797/71‑1 (MQ=255) gATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCa < 1:3876228/71‑1 (MQ=255) gATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCa < 1:3871501/71‑1 (MQ=255) gATAATGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGGGAATCCCTGGGCGATGGCGATATTGCGCa < 1:711674/71‑1 (MQ=255) gATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTgcgc < 1:455958/70‑1 (MQ=255) gATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTgcgc < 1:740513/70‑1 (MQ=255) aTGGCGTCGTAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCt < 1:3006064/70‑1 (MQ=255) aTGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCtt < 1:2040315/71‑1 (MQ=255) gCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGc < 1:2219166/70‑1 (MQ=255) tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAgt < 1:956911/71‑1 (MQ=255) tcgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAgt < 1:3378969/71‑1 (MQ=255) gtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAgtgt < 1:1407304/71‑1 (MQ=255) gtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAgtgt < 1:1348372/71‑1 (MQ=255) tAACCTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGc < 1:2970671/60‑1 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggcga < 1:3473643/49‑1 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGggcgatggcga < 1:2697893/49‑1 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTgcgc > 1:1684958/1‑57 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAgtggc < 1:2396289/71‑3 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTc < 1:4083824/71‑1 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTc < 1:2992438/71‑1 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTc < 1:2940360/71‑1 (MQ=255) tAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTc < 1:2065268/71‑1 (MQ=255) ccTGACAGTAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGt < 1:1688736/71‑1 (MQ=255) ccTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGt > 1:2873947/1‑71 (MQ=255) aCAGTAGGCGCACGAAGTCGATGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGAcg > 1:1433214/1‑71 (MQ=255) aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGAcg > 1:3880485/1‑71 (MQ=255) aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGAcg > 1:3303571/1‑71 (MQ=255) aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGAc > 1:1693656/1‑70 (MQ=255) cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGa > 1:3083663/1‑65 (MQ=255) cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTg < 1:705557/71‑1 (MQ=255) | CGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGACCTGGATAATGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTG > minE/1326603‑1326739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |