| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,751,234 | G→T | 14.1% | intergenic (‑20/‑187) | yfiF ← / → trxC | predicted methyltransferase/thioredoxin 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,751,234 | 0 | G | T | 14.1% | 127.3 / 5.1 | 42 | intergenic (‑20/‑187) | yfiF/trxC | predicted methyltransferase/thioredoxin 2 |
| Reads supporting (aligned to +/- strand): ref base G (15/21); new base T (2/4); total (17/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGAC > minE/1751164‑1751299 | gACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTg < 1:1723098/71‑1 (MQ=255)gACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTg < 1:2946305/71‑1 (MQ=255) tacatCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGcgcg < 1:1093848/71‑1 (MQ=255) catCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGaa < 1:1592095/71‑1 (MQ=255) catCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGaa < 1:3953822/71‑1 (MQ=255) catCACTTTGACCTTGCCACTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGa < 1:2649637/70‑1 (MQ=255) atCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAg > 1:846073/1‑71 (MQ=255) atCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAg < 1:2525412/71‑1 (MQ=255) aCTTTGCCCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGaa > 1:2392036/1‑67 (MQ=255) tGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAgcgcg > 1:412823/1‑68 (MQ=255) tGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAgcgcg > 1:3696680/1‑68 (MQ=255) gACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAg < 1:3742288/70‑1 (MQ=255) ttGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCa < 1:3742242/65‑1 (MQ=255) ttGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCa < 1:1776249/65‑1 (MQ=255) ttGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTAc > 1:3001297/1‑71 (MQ=255) tGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTAcc > 1:991126/1‑71 (MQ=255) gCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTAccc > 1:404084/1‑71 (MQ=255) cGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCg < 1:1853147/70‑1 (MQ=255) tttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTc > 1:1715031/1‑71 (MQ=255) ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTTcgcg < 1:945922/47‑1 (MQ=255) ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTTcgcg < 1:3701084/47‑1 (MQ=255) ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTTcgcg < 1:1746722/47‑1 (MQ=255) ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTTcgcg < 1:380911/47‑1 (MQ=255) ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGcgcg < 1:191080/47‑1 (MQ=255) ttACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTc < 1:3156055/70‑1 (MQ=255) aCCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTTcgcgca > 1:2056772/1‑45 (MQ=255) aCCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTTcgcgca > 1:880840/1‑45 (MQ=255) cTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcgcgc < 1:1452699/71‑1 (MQ=255) ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCgg > 1:2225610/1‑71 (MQ=255) tcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGt > 1:222325/1‑71 (MQ=255) atttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGaa > 1:1671908/1‑58 (MQ=255) atttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGtt < 1:4070977/70‑1 (MQ=255) atttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGtt < 1:420168/70‑1 (MQ=255) tttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcgcgc < 1:717391/65‑1 (MQ=255) ttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCg < 1:2979130/70‑1 (MQ=255) tcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTAc > 1:1991304/1‑50 (MQ=255) catCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGcc < 1:1017718/70‑1 (MQ=255) catCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCa > 1:2818948/1‑71 (MQ=255) atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcg < 1:811607/58‑1 (MQ=255) gTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGa < 1:613259/70‑1 (MQ=255) gTTCATGCTTTTCTCCACCAGCGCTGAGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGa < 1:3769050/70‑1 (MQ=255) aTGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCg < 1:3861313/71‑1 (MQ=255) ccaGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc > 1:1541140/1‑71 (MQ=255) cgcTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGAc > 1:2575885/1‑70 (MQ=255) | GACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGAC > minE/1751164‑1751299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |