| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,823,089 | A→G | 9.6% | L320L (TTA→TTG) | norW → | NADH:flavorubredoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,823,089 | 0 | A | G | 9.6% | 136.5 / 6.6 | 52 | L320L (TTA→TTG) | norW | NADH:flavorubredoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (28/19); new base G (2/3); total (30/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGAACGC > minE/1823022‑1823152 | aTCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCg > 1:1269668/1‑71 (MQ=255) tcttctCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTGCCGc > 1:202922/1‑71 (MQ=255) ttctCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTg > 1:93463/1‑71 (MQ=255) ttctCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTg > 1:1808349/1‑71 (MQ=255) ttctCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTg > 1:1589819/1‑71 (MQ=255) tctcGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTAc < 1:1360882/65‑1 (MQ=255) tAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATc < 1:1060730/65‑1 (MQ=255) aaCACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCa > 1:2234597/1‑69 (MQ=255) acacGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGc > 1:2070355/1‑71 (MQ=255) acGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGcta < 1:2585401/71‑3 (MQ=255) acGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGa < 1:1507083/71‑1 (MQ=255) cgcTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAAcc > 1:781522/1‑71 (MQ=255) cgcTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAAcc < 1:2075290/71‑1 (MQ=255) cgcTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAAcc > 1:2099300/1‑71 (MQ=255) cgcTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAAc < 1:323172/70‑1 (MQ=255) gAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGc > 1:1591159/1‑71 (MQ=255) gAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGc > 1:2108872/1‑71 (MQ=255) aaaCTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCg > 1:3647007/1‑71 (MQ=255) aaaCTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCCCTGCATCTGGCAGGCGAAACCCAGCg > 1:4119880/1‑71 (MQ=255) aaCTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTGCCGCTGCATCTGGCAGGCGAAACCCAGCGt > 1:175388/1‑71 (MQ=255) cccGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCATGCGAAACCCAGCGTCAg > 1:235925/1‑70 (MQ=255) ccGGCGATGCTGGTGAAAATCAAAACGCCGGAATTGCCGCTGCATCTGGCAGGCGAAACCCAGCGTCAg < 1:967395/69‑1 (MQ=255) ccGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAgg < 1:2705618/70‑1 (MQ=255) cGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAt > 1:3805540/1‑71 (MQ=255) cGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAt > 1:3417501/1‑71 (MQ=255) ggCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt < 1:4024323/71‑1 (MQ=255) ggCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt > 1:78357/1‑71 (MQ=255) ggCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt < 1:1167939/71‑1 (MQ=255) cGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTa > 1:3915324/1‑71 (MQ=255) tGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGc < 1:629891/71‑1 (MQ=255) gCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGAGAAACCCAGCGTCAGGATTTACGCt > 1:3146888/1‑71 (MQ=255) cTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTg > 1:1437981/1‑71 (MQ=255) cTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTg > 1:465125/1‑71 (MQ=255) ggTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTgg > 1:3112475/1‑70 (MQ=255) gTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTgg < 1:1410300/69‑1 (MQ=255) gTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTgg < 1:3155043/69‑1 (MQ=255) gTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGc > 1:579475/1‑70 (MQ=255) tGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCt > 1:2983975/1‑66 (MQ=255) aaTCAAAACGCCGGAATTGCCGCTGCATCTGGCAGGCGAAACCCAGc < 1:2584354/47‑1 (MQ=255) tCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTaa > 1:1821486/1‑70 (MQ=255) tCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAAt < 1:3202004/71‑1 (MQ=255) cAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAAccc > 1:1052171/1‑41 (MQ=255) aaaaCGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATAc < 1:3132585/71‑1 (MQ=255) aaCGCCGGAATTACCGCTGCATCTGGCATGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCg < 1:540771/71‑1 (MQ=255) aaCGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCg < 1:2105474/71‑1 (MQ=255) cGGAATTGCCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGAACGc < 1:1692123/71‑1 (MQ=255) gAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt < 1:3229901/43‑1 (MQ=255) gAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt < 1:316312/43‑1 (MQ=255) gAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt < 1:2392666/43‑1 (MQ=255) gAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATAc > 1:2209607/1‑62 (MQ=255) gAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATAc > 1:2418536/1‑62 (MQ=255) gAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATAc > 1:2352524/1‑62 (MQ=255) aCCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAAt > 1:1945300/1‑55 (MQ=255) | ATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGAACGC > minE/1823022‑1823152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |