| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,253,725 | C→T | 15.1% | A1105T (GCG→ACG) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,253,725 | 0 | C | T | 15.1% | 232.0 / 29.1 | 86 | A1105T (GCG→ACG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (38/35); new base T (5/8); total (43/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GATTCCTGCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCA > minE/2253655‑2253795 | gATTCCTGCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGc > 1:1180354/1‑71 (MQ=255)gATTCCTGCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGc > 1:2178440/1‑71 (MQ=255) ttCCTGCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTTCAGCTGAACAATCGCtt > 1:195302/1‑71 (MQ=255) ttCCTGCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGttt > 1:3509953/1‑71 (MQ=255) cTGCGGAATACGCGCCAGGGTGTCACCAGATCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTAc > 1:2299246/1‑71 (MQ=255) gCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTa > 1:1268353/1‑68 (MQ=255) cGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCg > 1:706410/1‑71 (MQ=255) ggAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCgg < 1:2111944/71‑1 (MQ=255) ggAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCgg > 1:2078582/1‑71 (MQ=255) aTACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCa < 1:480087/70‑1 (MQ=255) tACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg < 1:3830278/71‑1 (MQ=255) tACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg < 1:3830283/71‑1 (MQ=255) cgcgCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg > 1:1663411/1‑69 (MQ=255) cgcgCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg > 1:436468/1‑69 (MQ=255) cgcgCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg > 1:744639/1‑69 (MQ=255) cgcgCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg > 1:3889197/1‑69 (MQ=255) cgcgCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGa < 1:3471915/70‑1 (MQ=255) cgcgCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGa < 1:1165963/70‑1 (MQ=255) agcgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg > 1:3159559/2‑69 (MQ=255) gcgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg < 1:3521941/71‑1 (MQ=255) gcgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg > 1:2450595/1‑71 (MQ=255) gcgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg > 1:459451/1‑71 (MQ=255) gcgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg > 1:464357/1‑71 (MQ=255) cgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg > 1:3469598/1‑70 (MQ=255) cgcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGt < 1:2020902/71‑1 (MQ=255) gcCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGaa > 1:1871369/1‑68 (MQ=255) gtCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg < 1:796267/61‑1 (MQ=255) gtCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAg < 1:118371/61‑1 (MQ=255) gtCACAAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTACCCGGCAGGAAGTACTGCGCTg > 1:4083460/1‑71 (MQ=255) tCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTa < 1:3613206/48‑1 (MQ=255) tCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTa < 1:4056580/48‑1 (MQ=255) tCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTa < 1:1544809/48‑1 (MQ=255) tCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTa < 1:263315/48‑1 (MQ=255) tCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg < 1:1967683/57‑1 (MQ=255) tCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGt < 1:1412254/61‑1 (MQ=255) aCCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTAcc > 1:2964414/1‑48 (MQ=255) aCCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGTTTTAcc > 1:1583440/1‑48 (MQ=255) aCCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCCGCAGGAAGt < 1:1506028/59‑1 (MQ=255) ccAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCa > 1:202006/1‑70 (MQ=255) cAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCata > 1:3714008/1‑71 (MQ=255) agagCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCatat < 1:2597266/71‑1 (MQ=255) gagCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGaa > 1:204650/1‑53 (MQ=255) gagCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTgg < 1:395736/65‑1 (MQ=255) gagCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTgg < 1:2688190/65‑1 (MQ=255) gagCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGATGGCATATc > 1:3373879/1‑71 (MQ=255) agCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTgg < 1:3397865/64‑1 (MQ=255) agCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCg < 1:1974857/71‑1 (MQ=255) cTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAg < 1:4113501/47‑1 (MQ=255) cTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCgg > 1:3363968/1‑70 (MQ=255) cTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGt < 1:1609867/71‑1 (MQ=255) tGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTa > 1:2692015/1‑71 (MQ=255) gATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTAc > 1:3474260/1‑71 (MQ=255) gATCTGTACGCCATCTTCCAGCTGAACAATAGCTTTACCCGGCAGGAAGTACTGCGCTgg < 1:2683027/60‑1 (MQ=255) aTCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTa > 1:276730/1‑69 (MQ=255) tGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTa < 1:3194099/66‑1 (MQ=255) aCGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCa > 1:1743743/1‑37 (MQ=255) cGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTAc > 1:3651838/1‑44 (MQ=255) gCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCa < 1:3400295/71‑1 (MQ=255) ccATCTTCCAGCTGAACAATCGTTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAg < 1:2557460/71‑1 (MQ=255) ccATCTTCCAGCTGAACAATCGCTTTACCCGGCAgg > 1:3514044/1‑36 (MQ=255) cATCTTCCAGCTGAACAATCGCTTTGCCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGa < 1:2012734/71‑1 (MQ=255) cATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGa < 1:3058345/71‑1 (MQ=255) aTCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTgg < 1:1930532/48‑1 (MQ=255) aTCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAg < 1:1389032/69‑1 (MQ=255) tCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAc > 1:1918618/1‑71 (MQ=255) cTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcg > 1:1682085/1‑71 (MQ=255) tCCAGCTGAACAATCGTTTTACCCGGCAGGAAGTACTGCGCTgg > 1:534677/1‑44 (MQ=255) tCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCt > 1:2165551/1‑42 (MQ=255) ccAGCTGAAGAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcgtcg > 1:3585223/1‑71 (MQ=255) ccAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcgtcg > 1:4063877/1‑71 (MQ=255) ccAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcgtc < 1:697059/70‑1 (MQ=255) ccAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcgtc < 1:1868320/70‑1 (MQ=255) ccAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcg < 1:244123/68‑1 (MQ=255) ccAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAc < 1:1817067/67‑1 (MQ=255) cAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCg > 1:3594654/1‑49 (MQ=255) cAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTAc > 1:3522503/1‑53 (MQ=255) cAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTAc > 1:2766134/1‑53 (MQ=255) cAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTAc > 1:900495/1‑53 (MQ=255) aGCTGAACAATCGTTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAcgtcgt > 1:620272/1‑70 (MQ=255) gCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAAAGTCGTTa < 1:1355955/71‑1 (MQ=255) cTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTa < 1:3761809/70‑1 (MQ=255) aaCAATCGTTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTAcc < 1:3607064/69‑1 (MQ=255) aaCAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCt > 1:623088/1‑71 (MQ=255) cAATCGTTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGa < 1:474892/71‑1 (MQ=255) aTCGTTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAg < 1:1058404/53‑1 (MQ=255) aTCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAg > 1:2952229/1‑70 (MQ=255) tCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCa < 1:1532491/71‑1 (MQ=255) gCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCAt > 1:3350966/1‑70 (MQ=255) gCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATc < 1:312922/71‑1 (MQ=255) cTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCa < 1:2205306/71‑1 (MQ=255) | GATTCCTGCGGAATACGCGCCAGGGTGTCACCAGAGCTGATCTGTACGCCATCTTCCAGCTGAACAATCGCTTTACCCGGCAGGAAGTACTGCGCTGGCATATCGGTACCTGGGATCAGAACGTCGTTACCCTGAGCATCA > minE/2253655‑2253795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |