| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,331,736 | C→T | 6.9% | S208N (AGC→AAC) | fdhD ← | formate dehydrogenase formation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,331,736 | 0 | C | T | 6.9% | 373.8 / 13.0 | 115 | S208N (AGC→AAC) | fdhD | formate dehydrogenase formation protein |
| Reads supporting (aligned to +/- strand): ref base C (66/41); new base T (4/4); total (70/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
CACATCGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCATGCCC > minE/2331667‑2331806 | cacaTCGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCt > 1:3451955/1‑71 (MQ=255) acaTCGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCtt > 1:3574946/1‑71 (MQ=255) tCGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTcc > 1:1772590/1‑71 (MQ=255) cGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTccc > 1:3813449/1‑71 (MQ=255) cGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTccc > 1:1915779/1‑71 (MQ=255) cGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCctt > 1:361760/1‑71 (MQ=255) cGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCctt > 1:1835755/1‑71 (MQ=255) gACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:3148712/71‑1 (MQ=255) cTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttctt < 1:276145/71‑1 (MQ=255) cTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttctt > 1:145558/1‑71 (MQ=255) ttttGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTcc > 1:449595/1‑63 (MQ=255) ttttGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTtg > 1:542750/1‑71 (MQ=255) tGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTtgtg < 1:906628/70‑1 (MQ=255) tGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGa > 1:1290177/1‑71 (MQ=255) tGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGa > 1:435656/1‑71 (MQ=255) gCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCctt > 1:2664594/1‑63 (MQ=255) ccATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:762105/61‑1 (MQ=255) cATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCttt > 1:799525/1‑53 (MQ=255) tttCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGACAGCTATCCCCTTCTTGTGAc > 1:1638786/1‑65 (MQ=255) ttCATAACTGTCACGACTGGAAACCAGTACCGCACCTTGCTGCCATCTTTCCCCTTCTTGTGACcggcgg < 1:2436186/70‑1 (MQ=255) ttCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACcgg < 1:370275/67‑1 (MQ=255) tCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGcc > 1:277272/1‑71 (MQ=255) cATAACTGGCACGACTGGAAACCAGTACTGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAcc > 1:1048777/1‑63 (MQ=255) atcTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTa < 1:2036577/67‑1 (MQ=255) aaCTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTcccc > 1:3614803/1‑49 (MQ=255) ggCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:908145/48‑1 (MQ=255) gCACGACTGGAAACCAGTACCTCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAc > 1:3313243/1‑54 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGa > 1:2332240/1‑53 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAc > 1:1841499/1‑54 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAc > 1:89859/1‑54 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGttt < 1:1457689/71‑1 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGttt > 1:1849901/1‑71 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGttt < 1:774381/71‑1 (MQ=255) gCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGtt < 1:3905759/70‑1 (MQ=255) cACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTg < 1:2174037/71‑1 (MQ=255) aaCGACTGGAAACCAGTACCGCACCTTGCTGCCAGTTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTg < 1:2022655/70‑1 (MQ=255) cGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:2468088/44‑1 (MQ=255) cGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:1550522/44‑1 (MQ=255) cGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTtgtg < 1:1105628/49‑1 (MQ=255) aCTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTTTGa > 1:1977239/1‑48 (MQ=255) aCTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAc > 1:600320/1‑49 (MQ=255) aCTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAc > 1:1117043/1‑49 (MQ=255) aCTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGAc > 1:1773643/1‑49 (MQ=255) aCTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGt < 1:973454/64‑1 (MQ=255) aCTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCa < 1:422822/71‑1 (MQ=255) tGGAAACCAGTACCGCACCTTGCTGCCAGTTTTCCCcttct > 1:1047021/1‑41 (MQ=255) tGGAAACCAGTACCGCACCTTGCTGCCAGTTTTCCCcttct > 1:2400467/1‑41 (MQ=255) tGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAgc > 1:2333392/1‑71 (MQ=255) ggAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAgcg > 1:3807887/1‑71 (MQ=255) gAAACCATTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:1927279/38‑1 (MQ=255) gAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCcttc < 1:737948/38‑1 (MQ=255) gAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAgc < 1:3560176/69‑1 (MQ=255) aaaCCAGTACCGCACCTTGCTGCCAGCTTTCCCcttct < 1:2734029/38‑1 (MQ=255) aaaCCAGTACCGCACCTTGCTGCCAGCTTTCCCcttct < 1:1258787/38‑1 (MQ=255) aaaCCAGTACCGCACCTTGCTGCCAGCTTTCCCcttct < 1:2916118/38‑1 (MQ=255) aaaCCAGTACCGCACCTTGCTGCCAGCTTTCCCcttct < 1:518563/38‑1 (MQ=255) aCCAGTACCGCACCTTGCTGCCAGTTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGACCAGCGCca > 1:1000365/1‑70 (MQ=255) aCCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCca > 1:989709/1‑70 (MQ=255) aCCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCca > 1:1186005/1‑70 (MQ=255) aCCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCca > 1:825658/1‑70 (MQ=255) cAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGtt > 1:4043930/1‑56 (MQ=255) cAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACAt < 1:363817/71‑1 (MQ=255) cAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACAt < 1:2201815/71‑1 (MQ=255) aGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCggcggc > 1:3178384/1‑46 (MQ=255) aGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTaa > 1:3008798/1‑50 (MQ=255) gTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATgg > 1:3447728/1‑71 (MQ=255) gTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATgg > 1:1540043/1‑71 (MQ=255) gTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATg > 1:2412415/1‑70 (MQ=255) tACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGtt > 1:444952/1‑53 (MQ=255) tACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGt < 1:761509/52‑1 (MQ=255) tACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCa < 1:4017574/59‑1 (MQ=255) aCCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGc > 1:283724/1‑70 (MQ=255) ccGCACGTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGttt > 1:3884762/1‑52 (MQ=255) ccGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGttt > 1:1405225/1‑52 (MQ=255) ccGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCcaca > 1:1927488/1‑65 (MQ=255) ccGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCg < 1:3353191/70‑1 (MQ=255) ccGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGa < 1:2013326/71‑1 (MQ=255) ccGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCAGCCTAACAGTTTGTCCAGCGCCACATGGCg < 1:2637819/70‑1 (MQ=255) cGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGAc > 1:2672942/1‑71 (MQ=255) gCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGAc > 1:2320481/1‑70 (MQ=255) gCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGAc > 1:3233027/1‑70 (MQ=255) gCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGAc > 1:3057376/1‑70 (MQ=255) cACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGttt < 1:1960179/49‑1 (MQ=255) cACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACAt > 1:2347344/1‑63 (MQ=255) cTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAg < 1:3858869/43‑1 (MQ=255) tgctgcCTGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCca < 1:3441692/55‑1 (MQ=255) tgctgcCAGTTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTc > 1:2163415/1‑71 (MQ=255) tgctgcCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGtt > 1:2662725/1‑43 (MQ=255) ctgcCAGTTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCtt < 1:3246759/71‑1 (MQ=255) ctgcCAGTTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCtt < 1:3685717/71‑1 (MQ=255) gcCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGt < 1:1634702/38‑1 (MQ=255) ccAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCa < 1:2487443/70‑1 (MQ=255) ccAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCa < 1:3621604/70‑1 (MQ=255) ccAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCAt > 1:535382/1‑71 (MQ=255) ccAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCAt > 1:2474336/1‑71 (MQ=255) aGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTc > 1:1545011/1‑40 (MQ=255) aGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTc > 1:1434210/1‑40 (MQ=255) cTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCATGccc > 1:1811461/1‑71 (MQ=255) cTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCATGcc > 1:472672/1‑70 (MQ=255) cTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCATGc < 1:2007397/69‑1 (MQ=255) | CACATCGCCGACTTTTGCACCATTTCATAACTGGCACGACTGGAAACCAGTACCGCACCTTGCTGCCAGCTTTCCCCTTCTTGTGACCGGCGGCCTAACAGTTTGTCCAGCGCCACATGGCGACCCACGTCTTCATGCCC > minE/2331667‑2331806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |