| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,344,483 | C→A | 23.5% | G383G (GGC→GGA) | glnG → | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,344,483 | 0 | C | A | 23.5% | 186.2 / 28.6 | 68 | G383G (GGC→GGA) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Reads supporting (aligned to +/- strand): ref base C (27/25); new base A (10/6); total (37/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GAAAACACCTGCCGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTG > minE/2344415‑2344539 | gAAAACACCTGCCGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCg < 1:1638890/70‑1 (MQ=255) agacCTGCCGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTg > 1:3385180/3‑71 (MQ=255) gCCGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTg > 1:2673510/1‑65 (MQ=255) gCCGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGaa > 1:1094965/1‑71 (MQ=255) ccGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAAt < 1:2571337/71‑1 (MQ=255) gCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAAc < 1:2525010/69‑1 (MQ=255) tAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGa < 1:813123/61‑1 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGtt > 1:3732849/1‑70 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGtt > 1:629252/1‑70 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGtt > 1:1196459/1‑70 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTg > 1:2677997/1‑71 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTg > 1:2561977/1‑71 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTg > 1:1150433/1‑71 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTg > 1:1599513/1‑71 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGAGAACTGtt > 1:2972102/1‑57 (MQ=255) aaCGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGAGAACTGtt > 1:962422/1‑57 (MQ=255) cGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCg > 1:1366611/1‑71 (MQ=255) tGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCg < 1:646433/68‑1 (MQ=255) tGATGGCAGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCg > 1:548735/1‑68 (MQ=255) ggCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTa > 1:377426/1‑71 (MQ=255) gccgGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTc < 1:457268/71‑1 (MQ=255) cgGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGCACTGTTTGAATCAACGGTTGCGGAGAGTACTTCg > 1:2990214/1‑70 (MQ=255) cgGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGa > 1:2653006/1‑45 (MQ=255) cgGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCg > 1:2030540/1‑70 (MQ=255) gggCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCa < 1:189541/71‑1 (MQ=255) gggCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCa < 1:1450631/71‑1 (MQ=255) gggCAGGAAGTGTTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCa < 1:3831962/71‑1 (MQ=255) ggCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCa > 1:1330376/1‑70 (MQ=255) ggCAGGAAGTGTTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGagag < 1:802940/61‑1 (MQ=255) gCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaaa < 1:1413903/71‑1 (MQ=255) gCAGGAAGTGTTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaa < 1:1486536/70‑1 (MQ=255) cAGGAAGTGTTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTa > 1:3332550/1‑61 (MQ=255) ggAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGc < 1:2473387/71‑1 (MQ=255) ggAAGTGTTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaaa > 1:411947/1‑68 (MQ=255) gtgtTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGct < 1:1333150/48‑2 (MQ=39) tgtTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGgag < 1:2561725/50‑1 (MQ=255) tgtTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGgag < 1:333547/50‑1 (MQ=255) tgtTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGgag < 1:3640450/50‑1 (MQ=255) tgtTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCg < 1:3934263/71‑1 (MQ=255) tgtTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCg < 1:884892/71‑1 (MQ=255) gtTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACg < 1:2227567/40‑1 (MQ=255) gtTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTc > 1:619174/1‑57 (MQ=255) gtTGATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTc > 1:2352303/1‑57 (MQ=255) ttGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGga < 1:3805592/47‑1 (MQ=255) ttGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGga < 1:1955884/47‑1 (MQ=255) ttGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAAt > 1:3238741/1‑62 (MQ=255) ttGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGa > 1:985650/1‑71 (MQ=255) tGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTc > 1:1087247/1‑55 (MQ=255) tGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGa > 1:792862/1‑70 (MQ=255) gATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGa > 1:4068833/1‑69 (MQ=255) gATTCAGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaa < 1:946626/58‑1 (MQ=255) ttCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaa < 1:3007342/56‑1 (MQ=255) ttCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAg < 1:3297955/70‑1 (MQ=255) ttCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGc < 1:3007057/71‑1 (MQ=255) tCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGc < 1:2267561/59‑1 (MQ=255) tCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTT‑CGGAGAGTACTTCGCAAATGc < 1:1222586/58‑1 (MQ=255) ctggATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATg > 1:22312/3‑57 (MQ=255) cAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATg > 1:3414430/1‑57 (MQ=255) aGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGgaga > 1:1503232/1‑42 (MQ=255) aGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCaa > 1:322368/1‑59 (MQ=255) aGGATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTg > 1:2478542/1‑70 (MQ=255) ggATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGagag < 1:3413460/42‑1 (MQ=255) ggATTTGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTg < 1:3457969/69‑1 (MQ=255) tttGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCg < 1:1511029/46‑1 (MQ=255) tttGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaaa > 1:2598599/1‑50 (MQ=255) tttGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaaa > 1:2833514/1‑50 (MQ=255) tttGCCCGGAGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCaaa > 1:2027608/1‑50 (MQ=255) tGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCa > 1:2545823/1‑46 (MQ=255) | GAAAACACCTGCCGCTGGCTAACGGTGATGGCCGCCGGGCAGGAAGTGTTGATTCAGGATTTGCCCGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGACAGCTG > minE/2344415‑2344539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |