| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,417,803 | T→C | 14.4% | intergenic (‑77/+114) | yifK ← / ← rffM | predicted transporter/UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,417,803 | 0 | T | C | 14.4% | 315.8 / 43.3 | 125 | intergenic (‑77/+114) | yifK/rffM | predicted transporter/UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Reads supporting (aligned to +/- strand): ref base T (40/67); new base C (5/13); total (45/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
GTGTTGTGTTTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGAC > minE/2417735‑2417872 | gtgttgtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGtttt < 1:4029679/70‑1 (MQ=255)gtgttgtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGttt < 1:1373115/69‑1 (MQ=255)gtgttgtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGttt < 1:3257375/69‑1 (MQ=255)gtgttgtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTc > 1:3116204/1‑71 (MQ=255) tgtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGt < 1:3580065/69‑1 (MQ=255) tgtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTaa > 1:2915739/1‑71 (MQ=255) gtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAt > 1:4105174/1‑71 (MQ=255) gtgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAt > 1:213469/1‑71 (MQ=255) tgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAtt < 1:3686468/71‑1 (MQ=255) tgttTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAt < 1:1084413/70‑1 (MQ=255) tttGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCATATGTTTTCGTAATTc < 1:1647363/70‑1 (MQ=255) tttGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTc < 1:4048262/70‑1 (MQ=255) tttGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTc < 1:3398477/70‑1 (MQ=255) ttGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGttt < 1:589102/60‑1 (MQ=255) ttGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGttt < 1:2498377/60‑1 (MQ=255) cATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAg > 1:1014175/1‑68 (MQ=255) tGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgag < 1:797165/69‑1 (MQ=255) tGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGga < 1:4108130/68‑1 (MQ=255) tGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGga < 1:634487/68‑1 (MQ=255) tGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGga < 1:2167853/68‑1 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGaaa < 1:1640523/70‑1 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGaaa < 1:3253544/70‑1 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAAt > 1:2567882/1‑71 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAAt > 1:2196461/1‑71 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAAt < 1:3828448/71‑1 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAAt < 1:4030166/71‑1 (MQ=255) cTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAAt < 1:1215337/71‑1 (MQ=255) tttCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTc > 1:3639296/1‑54 (MQ=255) tCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATgg > 1:200712/1‑70 (MQ=255) cGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTaa > 1:3086153/1‑54 (MQ=255) cGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCa < 1:2046303/70‑1 (MQ=255) cGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCa < 1:3738722/70‑1 (MQ=255) gtgtTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:3833680/71‑1 (MQ=255) ttACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCaaa < 1:1305265/67‑1 (MQ=255) aCCGGTTATCGTTATTGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAg > 1:630284/1‑70 (MQ=255) aCCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:2282976/66‑1 (MQ=255) aCCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATa > 1:2620225/1‑67 (MQ=255) aCCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATa > 1:109348/1‑67 (MQ=255) aCCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATa > 1:233430/1‑67 (MQ=255) aCCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAg > 1:2990195/1‑70 (MQ=255) ccGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:3104906/65‑1 (MQ=255) ccGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:1313450/65‑1 (MQ=255) cGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGaaa > 1:2140761/1‑56 (MQ=255) cGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGaaa > 1:3740900/1‑56 (MQ=255) gTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCa > 1:83458/1‑47 (MQ=255) ttATCGTTATGGGTACATCGAGTGTTGCAAATGttt < 1:2951894/36‑1 (MQ=255) ttATCGTTATGGGTACATCGAGTGTTGCAAATGttt < 1:513781/36‑1 (MQ=255) ttATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCAg < 1:1006010/47‑1 (MQ=255) ttATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaa < 1:3959170/71‑1 (MQ=255) ttATCGTTATGGGTACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaa < 1:2874556/71‑1 (MQ=255) aTCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAtt < 1:2537772/42‑1 (MQ=255) aTCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAtt < 1:3841109/42‑1 (MQ=255) aTCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAAtt < 1:1511965/42‑1 (MQ=255) aTCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgaga < 1:460859/49‑1 (MQ=255) cGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaaaa < 1:3478483/70‑1 (MQ=255) gTTATGGGTACATCGAGTGTTGCAAATGTTTTCGt < 1:3149140/35‑1 (MQ=255) gTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAtt < 1:3477202/71‑1 (MQ=255) ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCa < 1:2844114/52‑1 (MQ=255) ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt < 1:3763398/71‑1 (MQ=255) ttATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt < 1:2160199/71‑1 (MQ=255) tATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgaga < 1:805843/44‑1 (MQ=255) tATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgaga < 1:2874517/44‑1 (MQ=255) tATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgaga < 1:157690/44‑1 (MQ=255) tATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgaga < 1:205525/44‑1 (MQ=255) aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCaaa > 1:438867/1‑52 (MQ=255) aTGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTAAGGAGAAATGGCaaa > 1:576549/1‑52 (MQ=255) tGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgag > 1:1722236/1‑41 (MQ=255) tGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGa < 1:362568/70‑1 (MQ=255) ggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGgaga < 1:2426476/40‑1 (MQ=255) ggTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGaaa > 1:1396572/1‑42 (MQ=255) gTACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGc < 1:3415812/71‑1 (MQ=255) tACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCt < 1:2170933/71‑1 (MQ=255) tACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCt < 1:288719/71‑1 (MQ=255) tACATCGAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCt < 1:3181432/71‑1 (MQ=255) cATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:2191262/46‑1 (MQ=255) cATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:3689900/46‑1 (MQ=255) cATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:1576948/46‑1 (MQ=255) cATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAAt < 1:194547/46‑1 (MQ=255) tCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAAttt < 1:2700643/60‑1 (MQ=255) tCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTtgt < 1:2525562/71‑1 (MQ=255) gAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTtgtg > 1:692934/1‑70 (MQ=255) gAGTGTTGCAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTgtgt < 1:776683/71‑1 (MQ=255) gtTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaa < 1:1022708/48‑1 (MQ=255) tGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTtgt > 1:4065456/1‑63 (MQ=255) gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa < 1:1465727/46‑1 (MQ=255) gCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa < 1:3867490/46‑1 (MQ=255) gCAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCtt > 1:680231/1‑59 (MQ=255) cAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAACGCATTTAAAAtt < 1:2495/49‑1 (MQ=255) cAAATGTTCTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATaaaa > 1:1376100/1‑70 (MQ=255) aaaTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTg < 1:1847561/50‑1 (MQ=255) aaTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg < 1:1839836/71‑1 (MQ=255) aTGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg > 1:4101802/1‑70 (MQ=255) tGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAg > 1:2647595/1‑69 (MQ=255) tGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCa < 1:1957080/71‑1 (MQ=255) ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa < 1:270381/39‑1 (MQ=255) ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa < 1:2983729/39‑1 (MQ=255) ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTaaa < 1:3357770/39‑1 (MQ=255) ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGc < 1:2308779/50‑1 (MQ=255) ttttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGc < 1:247248/50‑1 (MQ=255) ttCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGAc < 1:680726/70‑1 (MQ=255) | GTGTTGTGTTTGCATGCTTTCCGGTGTTACCGGTTATCGTTATGGGTACATCGAGTGTTGCAAATGTTTTCGTAATTCAGGAGAAATGGCAAATAAAGCATTAAAAATTTGAATGCTTTGTGTAATAAAAAAGCAGAC > minE/2417735‑2417872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |