BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,795,595	G→T	17.5%	E29* (GAA→TAA)	dgkA →	diacylglycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,795,595	0	G	T	17.5%	326.8 / 31.0	108	E29* (GAA→TAA)	dgkA	diacylglycerol kinase
Reads supporting (aligned to +/- strand): ref base G (43/46); new base T (6/13); total (49/59)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 111 total aligned reads displayed.

CTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGA  >  minE/2795527‑2795665

cTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACg                                                                        >  1:2395952/1‑69 (MQ=255)

   gATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAg                                                                     <  1:1488305/69‑1 (MQ=255)

   gATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAg                                                                     <  1:1928808/69‑1 (MQ=255)

   gATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAg                                                                     <  1:2217073/69‑1 (MQ=255)

   gATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCg                                                                   >  1:4104/1‑71 (MQ=255)

   gATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCg                                                                   >  1:1360407/1‑71 (MQ=255)

    aTTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCgg                                                                  >  1:431850/1‑71 (MQ=255)

    aTTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCgg                                                                  >  1:1637315/1‑71 (MQ=255)

      tCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCa                                                                >  1:2437882/1‑71 (MQ=255)

          ccGAATTATCAAAGCTGCAGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTcc                                                            <  1:3727517/71‑1 (MQ=255)

            gAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGt                                                          >  1:356890/1‑71 (MQ=255)

            gAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGt                                                          <  1:3397752/71‑1 (MQ=255)

            gAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGt                                                          <  1:3254697/71‑1 (MQ=255)

            gAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGAGGCATTCCGt                                                          >  1:3656059/1‑71 (MQ=255)

               ttATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAg                                                       <  1:2854721/71‑1 (MQ=255)

                tATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCgg                                                                  <  1:793974/59‑1 (MQ=255)

                  tCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCa                                                                >  1:1999629/1‑59 (MQ=255)

                    aaaGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAgg                                                  >  1:3501547/1‑71 (MQ=255)

                       gctgctGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGt                                               <  1:1025543/71‑1 (MQ=255)

                        ctgctgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCg                                                >  1:3345130/1‑69 (MQ=255)

                          gctgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGc                                            >  1:1384878/1‑71 (MQ=255)

                           ctgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCg                                           >  1:2478886/1‑71 (MQ=255)

                           ctgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCg                                           >  1:1726793/1‑71 (MQ=255)

                           ctgGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCg                                           >  1:2410225/1‑71 (MQ=255)

                              gCTATTCCTGTAAAGGTTTACGGGCTGCATGGATCAACGAAGCGGCAt                                                               <  1:3552204/48‑1 (MQ=255)

                              gCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAg                                                       <  1:727354/56‑1 (MQ=255)

                              gCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAg                                                       <  1:172976/56‑1 (MQ=255)

                              gCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGt                                         <  1:1924777/70‑1 (MQ=255)

                              gCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTa                                        >  1:2044914/1‑71 (MQ=255)

                                tATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAg                                             >  1:1107343/1‑64 (MQ=255)

                                tATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      >  1:2205216/1‑71 (MQ=255)

                                tATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      >  1:2299437/1‑71 (MQ=255)

                                 aTTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCAt                                                               >  1:2560435/1‑45 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCAt                                                               <  1:3766517/44‑1 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCAt                                                               <  1:2135441/44‑1 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      <  1:3703820/69‑1 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      <  1:3372091/69‑1 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgt                                    >  1:1224504/1‑71 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgt                                    >  1:884613/1‑71 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      <  1:1448453/69‑1 (MQ=255)

                                  ttCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      <  1:1285133/69‑1 (MQ=255)

                                   tCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgtt                                   >  1:3444459/1‑71 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCg                                                                   >  1:2156882/1‑38 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCg                                                                   >  1:250924/1‑38 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCg                                                                   >  1:2647836/1‑38 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgttg                                  >  1:1752074/1‑71 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgtt                                   <  1:3835530/70‑1 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgtt                                   <  1:631207/70‑1 (MQ=255)

                                    ccTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgtt                                   <  1:770048/70‑1 (MQ=255)

                                     cTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgttg                                  <  1:306755/70‑1 (MQ=255)

                                     cTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTgg                                 >  1:633623/1‑71 (MQ=255)

                                     cTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTgg                                 >  1:2883700/1‑71 (MQ=255)

                                      tGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTcc                                                            <  1:1778408/43‑1 (MQ=255)

                                      tGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgttg                                  <  1:3032547/69‑1 (MQ=255)

                                      tGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGc                                >  1:885980/1‑71 (MQ=255)

                                       ggAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGa                                                     <  1:870960/49‑1 (MQ=255)

                                       ggAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCTTAGCGGTATTGTTGGCg                               >  1:3353551/1‑71 (MQ=255)

                                       ggAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgt                                    >  1:2702978/1‑66 (MQ=255)

                                       ggAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCg                               >  1:3645183/1‑71 (MQ=255)

                                        gAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCg                               <  1:1735426/70‑1 (MQ=255)

                                         aaaGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggt                             >  1:797309/1‑71 (MQ=255)

                                         aaaGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggt                             >  1:5531/1‑71 (MQ=255)

                                         aaaGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggt                             <  1:3458170/71‑1 (MQ=255)

                                          aaGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCg                                           >  1:2490335/1‑56 (MQ=255)

                                          aaGGTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      <  1:3586575/61‑1 (MQ=255)

                                           aGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtgg                           >  1:4035994/1‑71 (MQ=255)

                                           aGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtgg                           <  1:2114242/71‑1 (MQ=255)

                                            ggTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtt                                      <  1:1042758/59‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAg                                                       <  1:3266848/41‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTc                         <  1:279243/71‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACTAAGCGGCATTCCGGCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTc                         <  1:2179454/71‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCgg                                          <  1:1162879/54‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCgg                                          <  1:3220989/54‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggtggt                          >  1:2275519/1‑70 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTc                         <  1:2707646/71‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTc                         <  1:764109/71‑1 (MQ=255)

                                             gTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTc                         <  1:2880640/71‑1 (MQ=255)

                                              tttACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCgg                              <  1:240007/65‑1 (MQ=255)

                                              tttACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCgg                              <  1:3301525/65‑1 (MQ=255)

                                              tttACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCa                        >  1:2636670/1‑71 (MQ=255)

                                                tACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCAt                       <  1:128977/70‑1 (MQ=255)

                                                  cgcgCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGc                    >  1:3911033/1‑71 (MQ=255)

                                                        gCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCctgc                <  1:516421/69‑1 (MQ=255)

                                                         cATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTgctgg             <  1:934632/71‑1 (MQ=255)

                                                           tGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCg                               <  1:3807727/51‑1 (MQ=255)

                                                            ggATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTgctggctg          >  1:3049734/1‑71 (MQ=255)

                                                             gATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTgctggctg          >  1:1960737/1‑70 (MQ=255)

                                                             gATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGctggctgg         <  1:1509096/71‑1 (MQ=255)

                                                               tCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCggt                             <  1:1098649/49‑1 (MQ=255)

                                                               tCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAt       <  1:3919795/71‑1 (MQ=255)

                                                               tCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAt       <  1:2272670/71‑1 (MQ=255)

                                                               tCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAt       <  1:1829341/71‑1 (MQ=255)

                                                               tCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAt       >  1:1278113/1‑71 (MQ=255)

                                                                 aaCGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAtgt     >  1:2602786/1‑71 (MQ=255)

                                                                  aCGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTTGTCATCGCCTGCTGGCTGGAtgtg    <  1:1882204/71‑1 (MQ=255)

                                                                  aCGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAtgtg    <  1:2771117/71‑1 (MQ=255)

                                                                   cGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAtgtg    >  1:3223272/1‑70 (MQ=255)

                                                                   cGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTgg   <  1:44441/71‑1 (MQ=255)

                                                                   cGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTgg   <  1:1156404/71‑1 (MQ=255)

                                                                    gAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGa  >  1:566783/1‑71 (MQ=255)

CTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGA  >  minE/2795527‑2795665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`