| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,817,653 | A→G | 7.2% | P258P (CCA→CCG) | nrfA → | nitrite reductase, formate‑dependent, cytochrome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,817,653 | 0 | A | G | 7.2% | 233.2 / 8.8 | 83 | P258P (CCA→CCG) | nrfA | nitrite reductase, formate‑dependent, cytochrome |
| Reads supporting (aligned to +/- strand): ref base A (39/38); new base G (4/2); total (43/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCT > minE/2817585‑2817723 | gAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCaa < 1:3209401/70‑1 (MQ=255)gAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAAt > 1:3286527/1‑71 (MQ=255)gAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAAt > 1:2905041/1‑71 (MQ=255) aaaTATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATg < 1:4047776/70‑1 (MQ=255) aaTATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCt < 1:1346247/71‑1 (MQ=255) aaTATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCt < 1:2375288/71‑1 (MQ=255) tatGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa < 1:3953330/71‑1 (MQ=255) tatGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa < 1:3600725/71‑1 (MQ=255) atGGAGCAGTATTAAGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCt < 1:1974422/68‑1 (MQ=255) tGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGaaa < 1:73764/71‑1 (MQ=255) aGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAgcg < 1:4042071/71‑1 (MQ=255) aGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAgcg < 1:2239684/71‑1 (MQ=255) aGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAgcg < 1:2975046/71‑1 (MQ=255) gCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAgcg < 1:3702113/70‑1 (MQ=255) tATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa < 1:3663386/61‑1 (MQ=255) tATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa < 1:218424/61‑1 (MQ=255) tATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa < 1:2290929/61‑1 (MQ=255) tATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa < 1:114285/61‑1 (MQ=255) ttACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCa < 1:3527908/52‑1 (MQ=255) ttACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCGATGCTGAAAGCgca > 1:3793347/1‑66 (MQ=255) ttACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCGATGCTGAAAGCgca > 1:858926/1‑66 (MQ=255) ttACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGc > 1:1243392/1‑56 (MQ=255) aCGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCGATGCTGAAAGCGCAGCACCCg > 1:2814798/1‑71 (MQ=255) aCGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCg > 1:1406366/1‑71 (MQ=255) aCGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCg > 1:1005533/1‑71 (MQ=255) aCGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCg > 1:936040/1‑71 (MQ=255) aCGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCg > 1:1163269/1‑71 (MQ=255) cGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCg < 1:1330173/70‑1 (MQ=255) gACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCgg > 1:1372048/1‑70 (MQ=255) gACAAAATTGCCT‑‑TCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAat < 1:1652844/71‑1 (MQ=255) aCAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGaa < 1:1280558/71‑1 (MQ=255) aCAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGaa < 1:2544144/71‑1 (MQ=255) cAAAATTGCCTTCTCTTACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGaa > 1:3289683/1‑70 (MQ=255) cAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCg < 1:3338866/67‑1 (MQ=255) cAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGaa < 1:1032216/70‑1 (MQ=255) cAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGaa > 1:80981/1‑70 (MQ=255) cAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGaa > 1:1294872/1‑70 (MQ=255) aaaaTTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAata > 1:1899087/1‑71 (MQ=255) aaaaTTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAata > 1:2498032/1‑71 (MQ=255) aaaTTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAatat < 1:3661853/71‑1 (MQ=255) aaTTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATg < 1:3169788/71‑1 (MQ=255) aaTTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATg < 1:2671822/71‑1 (MQ=255) ttGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGaa < 1:943314/71‑1 (MQ=255) cTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCGATGCTGAAAgcg < 1:388214/49‑1 (MQ=255) ttCTCTGACTGGACTAACTCCCTGTCGAAAACGCCGATGCTGAAAGCGCAGCACCCGGAATATGAAACCTg > 1:1491079/1‑71 (MQ=255) tctcTGACTGGACTAACTCCCTGTCGAAAACGCCAATg > 1:3379992/1‑38 (MQ=255) ctctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTg < 1:396463/69‑1 (MQ=255) ctctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTg < 1:2945246/69‑1 (MQ=255) ctctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGa > 1:3155985/1‑71 (MQ=255) ctctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGa > 1:2106484/1‑71 (MQ=255) tctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCgcag > 1:1338583/1‑48 (MQ=255) tctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCgcag > 1:1860034/1‑48 (MQ=255) tctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCgcag > 1:800823/1‑48 (MQ=255) tctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCgcag > 1:255607/1‑48 (MQ=255) tctGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGa > 1:1591137/1‑70 (MQ=255) tgacttgactAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAg > 1:2101561/1‑71 (MQ=255) gACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCgca > 1:1984732/1‑44 (MQ=255) aCTGGACTAACTCCCTGTCGAAAACGCCGATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAg < 1:743604/69‑1 (MQ=255) gACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCa < 1:2740383/71‑1 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGc > 1:1427986/1‑68 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCa < 1:3671617/69‑1 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCa < 1:1681001/69‑1 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCAtt < 1:775087/71‑1 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCAtt > 1:2269735/1‑71 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCAtt > 1:2624053/1‑71 (MQ=255) cTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCAtt > 1:230485/1‑71 (MQ=255) aCTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTc < 1:3092498/69‑1 (MQ=255) aCTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCa > 1:3910039/1‑70 (MQ=255) aCTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCAc > 1:3070418/1‑71 (MQ=255) tCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGa > 1:1102035/1‑55 (MQ=255) cTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGaa < 1:2232144/45‑1 (MQ=255) cTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTa > 1:2311299/1‑70 (MQ=255) gTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAAc > 1:971150/1‑45 (MQ=255) tCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTaaaaa > 1:96925/1‑71 (MQ=255) tCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTaaaaa < 1:3295755/71‑1 (MQ=255) gAAAACGCCAATGCTGAAAGCGCAGCACCCGGAata > 1:3158234/1‑36 (MQ=255) gAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCa > 1:412672/1‑60 (MQ=255) gAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAaaca < 1:1925816/71‑1 (MQ=255) gAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAaaca < 1:1938158/71‑1 (MQ=255) gAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAaac > 1:1606798/1‑70 (MQ=255) aaaaCGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTc < 1:1306865/58‑1 (MQ=255) aaCGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCAc > 1:3426427/1‑58 (MQ=255) aCGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGt < 1:2053077/71‑1 (MQ=255) ccAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGAc > 1:1905037/1‑71 (MQ=255) cAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGAcc > 1:768898/1‑71 (MQ=255) aaTGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCt < 1:2607568/71‑1 (MQ=255) | GAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTGACCT > minE/2817585‑2817723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |